Detailed information of component protein


Summary

Component ID T6CP000090
Component protein type TssK
T6SS ID (Type) T6SS00013 experimental (Type i1)
Strain Pseudomonas putida KT2440
Replicon chromosome
Sequence Protein sequence (446 a.a.); Nucleotide sequence (1338 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) PP_RS13645
Coordinate (Strand) 2993074..2994411 (-)
NCBI ID WP_010953537.1
RefSeq NC_002947
Uniprot ID Q88JN2_PSEPK, A0A2V4FMD2_9PSED
KEGG ID ppu:PP_2617
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
T6SS_VasE PF05936.14 4.4e-149 19..442
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-445 MSSRNPVLWPEGLFVKPQHFQQAARASEAALHQRLASLNAAFYGFSELQLNDEYLSLGKVAITRARGVMPDGTVFDIPADLPPPPPLEIGDDACQDSEVYLCLPLRTDGGREVSWPDNAANFRYTAQVQEIKDTHSADGDLVQVDLAVPNLQLKRKTEDSSAYTRLALARILERRPDGSLQLDEAFYPTSVSVRAVPALQRFLEEITHTLRERARGLATRIGASGQSGIADIRDFNLLQAMNRWWPCFQHLARQAQTHPEQLYLCMSQACGEFVTFTDQNRLPQEYPAYHHTALRTSFKPLEDTLRRSLSTVLRPRAVSLPLESEQFGVMTTTLEDRRLIDEADFILAVRASMPPQALRQMFIQKAKITSLESLNDLVPLQLPGIPLLPLPVAPRDLPFHAGFSYFELDRRDPAWACMSKANGFGIHIAGDFPGLELQFWAIRSE


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9653 - -


  Protein sequence: 446 a.a.    

>T6CP000090 NC_002947:c2994411-2993074 [Pseudomonas putida KT2440] [TssK]
MSSRNPVLWPEGLFVKPQHFQQAARASEAALHQRLASLNAAFYGFSELQLNDEYLSLGKVAITRARGVMPDGTVFDIPAD
LPPPPPLEIGDDACQDSEVYLCLPLRTDGGREVSWPDNAANFRYTAQVQEIKDTHSADGDLVQVDLAVPNLQLKRKTEDS
SAYTRLALARILERRPDGSLQLDEAFYPTSVSVRAVPALQRFLEEITHTLRERARGLATRIGASGQSGIADIRDFNLLQA
MNRWWPCFQHLARQAQTHPEQLYLCMSQACGEFVTFTDQNRLPQEYPAYHHTALRTSFKPLEDTLRRSLSTVLRPRAVSL
PLESEQFGVMTTTLEDRRLIDEADFILAVRASMPPQALRQMFIQKAKITSLESLNDLVPLQLPGIPLLPLPVAPRDLPFH
AGFSYFELDRRDPAWACMSKANGFGIHIAGDFPGLELQFWAIRSE*

  Nucleotide sequence: 1338 bp    

>T6CP000090 NC_002947:c2994411-2993074 [Pseudomonas putida KT2440] [TssK]
ATGAGTTCACGCAATCCCGTTCTCTGGCCCGAAGGCCTGTTCGTCAAACCACAGCATTTCCAACAGGCGGCCAGGGCCAG
CGAAGCAGCCCTGCACCAGCGCCTCGCCAGCCTCAATGCAGCCTTTTACGGCTTCAGCGAACTTCAGTTGAACGACGAAT
ACCTGAGCCTGGGCAAAGTCGCCATCACCCGGGCGCGAGGTGTGATGCCGGACGGCACGGTGTTCGACATTCCCGCCGAC
CTGCCGCCACCGCCGCCGCTGGAAATCGGCGACGACGCGTGCCAAGACAGTGAGGTGTATCTGTGCCTGCCGCTGCGTAC
CGATGGCGGCCGCGAAGTGAGCTGGCCCGACAATGCAGCCAATTTCCGCTATACCGCTCAGGTACAGGAAATCAAGGATA
CCCACTCCGCTGACGGTGACCTGGTGCAGGTAGACCTTGCGGTGCCCAACCTGCAGCTCAAACGCAAGACCGAAGACAGC
AGCGCCTACACCCGCCTGGCCCTGGCACGTATTCTGGAACGGCGCCCCGACGGCAGCCTGCAACTGGACGAAGCGTTCTA
CCCCACCAGCGTTTCGGTGCGGGCGGTGCCAGCGCTGCAGCGGTTTCTAGAGGAAATCACCCATACCTTGCGCGAGCGGG
CGCGCGGCCTTGCCACGCGGATTGGTGCCTCGGGCCAGTCCGGGATCGCCGACATTCGCGACTTCAACCTGCTGCAGGCC
ATGAACCGTTGGTGGCCATGCTTCCAGCATCTGGCCAGGCAAGCGCAAACGCACCCCGAGCAGCTGTACCTGTGCATGAG
CCAGGCCTGCGGCGAATTCGTCACCTTCACCGACCAGAACCGGCTGCCGCAGGAGTACCCCGCCTATCACCACACCGCAC
TGCGCACGTCTTTCAAACCGCTGGAAGATACCTTGCGCCGGTCTCTGAGCACCGTCTTGCGGCCACGCGCTGTTTCACTA
CCGCTGGAGAGCGAACAATTCGGCGTGATGACGACCACGCTGGAAGACCGCCGGCTGATCGACGAAGCCGACTTCATCCT
CGCCGTGCGCGCCAGCATGCCACCACAGGCGTTGCGCCAGATGTTCATCCAGAAAGCCAAGATCACCTCACTTGAGTCAC
TGAATGACCTGGTGCCGCTGCAATTGCCGGGCATCCCTTTGCTGCCATTACCCGTGGCGCCCCGCGACCTGCCCTTCCAT
GCGGGTTTCAGCTACTTCGAGCTGGACCGACGTGACCCGGCCTGGGCGTGCATGAGCAAGGCGAATGGCTTCGGCATCCA
CATTGCGGGCGACTTCCCAGGGCTTGAACTGCAGTTCTGGGCGATCAGGAGTGAGTGA