SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP000074
Component protein type	TssJ
T6SS ID (Type)	T6SS00009 (Type i3)
Strain	Bordetella bronchiseptica RB50
Replicon	chromosome
Sequence	Protein sequence (181 a.a.); Nucleotide sequence (543 bp)
Reference	[1] Weyrich LS, Rolin OY, Muse SJ, et al. A Type VI secretion system encoding locus is required for Bordetella bronchiseptica immunomodulation and persistence in vivo. PLoS One. 2012;7(10):e45892. doi: 10.1371/journal.pone.0045892. Epub 2012 Oct 12. PMID: 23071529 [2] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	AYT36_RS04115
Coordinate (Strand)	853426..853968 (+)
NCBI ID	WP_010925953.1
RefSeq	NC_002927
Uniprot ID	A0A0H3LI29_BORBR
KEGG ID	bbr:BB0813
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS-SciN	PF12790.9	1.9e-37	37..149

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-180	MLVAVPTRRALRAAAWIAVAVLAAGCASTASRKAAPYAIELKADPRVNPDASGRPSPIQVTLYELRSSAEFEARDFFTLEGDAQAALGKSLIETDQVILQPGETRKLSYPGNTEARVVGIVAAYRDLERSPWRIVVPLPQAQNTNIYKFWQFSPNEETVRVAVGGQGVQIVDRERSWWPF

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
LIPO(Sec/SPII)	0.9588	pos: 25-26. AAG-CA.	MLVAVPTRRALRAAAWIAVAVLAAG

Protein sequence: 181 a.a.

						>T6CP000074 NC_002927:853426-853968 [Bordetella bronchiseptica RB50] [TssJ]
MLVAVPTRRALRAAAWIAVAVLAAGCASTASRKAAPYAIELKADPRVNPDASGRPSPIQVTLYELRSSAEFEARDFFTLE
GDAQAALGKSLIETDQVILQPGETRKLSYPGNTEARVVGIVAAYRDLERSPWRIVVPLPQAQNTNIYKFWQFSPNEETVR
VAVGGQGVQIVDRERSWWPF*					

Nucleotide sequence: 543 bp

						>T6CP000074 NC_002927:853426-853968 [Bordetella bronchiseptica RB50] [TssJ]
TTGCTTGTTGCTGTGCCCACGCGGCGCGCGTTGCGCGCGGCGGCCTGGATTGCCGTCGCGGTGCTGGCCGCCGGGTGCGC
GTCCACGGCCAGCCGCAAGGCGGCGCCTTACGCGATCGAGCTGAAGGCCGATCCGCGCGTCAATCCCGATGCCTCGGGCC
GGCCCTCGCCGATCCAGGTCACGCTGTACGAGCTGCGTTCGTCCGCCGAGTTCGAGGCGCGCGATTTCTTCACCCTGGAA
GGCGACGCGCAGGCCGCGCTGGGCAAGTCGCTCATCGAAACCGATCAGGTCATCCTGCAGCCCGGCGAAACCCGCAAGCT
GTCGTATCCGGGCAATACCGAGGCGCGCGTGGTCGGCATCGTGGCGGCCTATCGCGACCTCGAGCGCAGTCCCTGGCGCA
TCGTCGTGCCCCTGCCGCAGGCCCAGAACACCAACATCTACAAGTTCTGGCAGTTCTCGCCCAACGAGGAGACCGTGCGC
GTCGCGGTGGGCGGACAGGGCGTGCAAATCGTCGACCGGGAGCGTTCCTGGTGGCCCTTCTGA					