SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP000070
Component protein type	TssE
T6SS ID (Type)	T6SS00009 (Type i3)
Strain	Bordetella bronchiseptica RB50
Replicon	chromosome
Sequence	Protein sequence (173 a.a.); Nucleotide sequence (519 bp)
Reference	[1] Weyrich LS, Rolin OY, Muse SJ, et al. A Type VI secretion system encoding locus is required for Bordetella bronchiseptica immunomodulation and persistence in vivo. PLoS One. 2012;7(10):e45892. doi: 10.1371/journal.pone.0045892. Epub 2012 Oct 12. PMID: 23071529 [2] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	AYT36_RS04070
Coordinate (Strand)	844093..844611 (+)
NCBI ID	WP_003808292.1
RefSeq	NC_002927
Uniprot ID	A0A0H3LI50_BORBR, Q7W1H1_BORPA, A0A5Q0XPA2_BORPP
KEGG ID	bbr:BB0804, bpa:BPP0718
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
GPW_gp25	PF04965.16	3.1e-21	43..146

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-172	MRIADERVARDRLQPSLLDRLVDDEPWRRSEAPEAALLTHAGLRAAVLRDLRWLLNTVNLASSQDLAPYRHVPASSVNFGVRAMAGRRMSEIDWVDVETSIRDAIAAFEPRILESSVEVRCVSDTRTLEHHNVLSLEIRGMLWCVPHPREFLFRTDIDLESGHMDLRDLGGA

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9898	-	-

Protein sequence: 173 a.a.

						>T6CP000070 NC_002927:844093-844611 [Bordetella bronchiseptica RB50] [TssE]
MRIADERVARDRLQPSLLDRLVDDEPWRRSEAPEAALLTHAGLRAAVLRDLRWLLNTVNLASSQDLAPYRHVPASSVNFG
VRAMAGRRMSEIDWVDVETSIRDAIAAFEPRILESSVEVRCVSDTRTLEHHNVLSLEIRGMLWCVPHPREFLFRTDIDLE
SGHMDLRDLGGA*					

Nucleotide sequence: 519 bp

						>T6CP000070 NC_002927:844093-844611 [Bordetella bronchiseptica RB50] [TssE]
ATGAGAATCGCCGACGAACGGGTGGCGCGCGACCGCCTGCAGCCGTCGCTGCTCGATCGCCTGGTCGATGACGAGCCATG
GCGCCGCAGCGAGGCGCCCGAAGCGGCGCTGTTGACGCACGCCGGGCTGCGCGCCGCGGTGCTGCGCGACCTGCGCTGGC
TGCTCAACACGGTCAACCTCGCCTCGTCGCAGGATCTGGCGCCGTACCGGCACGTGCCCGCCTCCAGCGTCAATTTCGGC
GTGCGCGCCATGGCCGGACGCCGCATGTCGGAGATCGACTGGGTCGATGTCGAGACGTCGATCCGCGACGCCATCGCGGC
GTTCGAGCCGCGCATCCTGGAATCCTCGGTCGAGGTGCGCTGCGTCAGCGACACCCGCACGCTCGAGCACCACAATGTGC
TCAGCCTGGAAATACGCGGCATGCTGTGGTGCGTGCCGCACCCGCGCGAGTTCCTGTTCCGCACCGATATCGACCTGGAA
AGCGGGCACATGGACCTGCGCGACCTGGGAGGGGCGTGA					