SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP000066
Component protein type	TssA
T6SS ID (Type)	T6SS00009 (Type i3)
Strain	Bordetella bronchiseptica RB50
Replicon	chromosome
Sequence	Protein sequence (343 a.a.); Nucleotide sequence (1029 bp)
Reference	[1] Weyrich LS, Rolin OY, Muse SJ, et al. A Type VI secretion system encoding locus is required for Bordetella bronchiseptica immunomodulation and persistence in vivo. PLoS One. 2012;7(10):e45892. doi: 10.1371/journal.pone.0045892. Epub 2012 Oct 12. PMID: 23071529 [2] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	AYT36_RS04045
Coordinate (Strand)	839536..840564 (+)
NCBI ID	WP_033446914.1
RefSeq	NC_002927
Uniprot ID	UPI00050091C2
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
ImpA_N	PF06812.14	9.4e-36	10..130

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-342	MEFADLLKTLTPSLPCGEDLEYDADFLQLQQAAVGRSEQQFGATIIPAQAPDWRAVERLALGLLERTRDLRIIAYLTRAWTEMRGLPGYAEGVTLAAGTLEQYWDAVHPMLASGGEEDPMPRVNALASLGDPQGCVRGMRSACLLDDVHGRLSLRDAEALLDGGRSEADYPGGRTRLIENLRQARLRRDDAALAVGAAAGALRRIQQQATQRLGSAWCPDYAQALRALDALLRTWSDLDETAAAPAAAMPAEAHHAAPAPEPQPAPASWHDARIATREDAMAMLAKVSAYFETHEPSHPAPYLIRRVQQLIPLDFHDILRNLAPQGLAQFEAWTAREAASQS

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9891	-	-

Protein sequence: 343 a.a.

						>T6CP000066 NC_002927:839536-840564 [Bordetella bronchiseptica RB50] [TssA]
MEFADLLKTLTPSLPCGEDLEYDADFLQLQQAAVGRSEQQFGATIIPAQAPDWRAVERLALGLLERTRDLRIIAYLTRAW
TEMRGLPGYAEGVTLAAGTLEQYWDAVHPMLASGGEEDPMPRVNALASLGDPQGCVRGMRSACLLDDVHGRLSLRDAEAL
LDGGRSEADYPGGRTRLIENLRQARLRRDDAALAVGAAAGALRRIQQQATQRLGSAWCPDYAQALRALDALLRTWSDLDE
TAAAPAAAMPAEAHHAAPAPEPQPAPASWHDARIATREDAMAMLAKVSAYFETHEPSHPAPYLIRRVQQLIPLDFHDILR
NLAPQGLAQFEAWTAREAASQS*					

Nucleotide sequence: 1029 bp

						>T6CP000066 NC_002927:839536-840564 [Bordetella bronchiseptica RB50] [TssA]
ATGGAATTTGCCGACCTGTTGAAGACGCTGACGCCCAGCCTGCCCTGCGGGGAGGACCTGGAGTACGACGCTGATTTCCT
GCAGTTGCAGCAGGCCGCCGTGGGCCGCAGCGAGCAGCAGTTCGGCGCCACCATCATCCCCGCGCAGGCGCCCGACTGGC
GCGCGGTGGAGCGGCTGGCGCTGGGGCTGCTGGAGCGCACGCGCGACCTGCGCATCATCGCCTACCTGACGCGCGCCTGG
ACCGAGATGCGCGGCCTGCCCGGCTATGCCGAGGGCGTGACGCTGGCCGCCGGCACGCTGGAGCAATACTGGGACGCGGT
GCATCCGATGCTGGCCAGCGGCGGCGAGGAAGACCCGATGCCGCGCGTCAATGCGCTGGCCTCGCTGGGCGACCCGCAGG
GCTGCGTGCGCGGCATGCGCTCGGCCTGCCTGCTCGACGATGTGCATGGCCGCCTGTCGCTGCGCGATGCCGAGGCCCTG
CTCGACGGCGGCAGGAGCGAGGCCGACTATCCGGGCGGGCGCACGCGCCTGATCGAGAACCTGCGCCAGGCGCGCCTGCG
GCGCGACGACGCGGCGCTGGCCGTGGGCGCGGCCGCCGGCGCGCTGCGCCGCATCCAGCAGCAGGCCACCCAGCGCCTGG
GCTCGGCCTGGTGTCCGGACTATGCGCAGGCGCTGCGCGCGCTGGATGCCTTGCTGCGCACCTGGAGCGACCTGGACGAA
ACCGCGGCGGCGCCCGCCGCGGCCATGCCGGCCGAAGCGCATCACGCGGCCCCGGCGCCTGAACCCCAACCCGCGCCCGC
CAGCTGGCACGACGCGCGCATCGCCACGCGCGAAGACGCGATGGCCATGCTGGCCAAGGTCAGCGCCTACTTCGAAACCC
ATGAGCCCAGCCATCCGGCGCCCTATCTGATCCGCCGGGTGCAGCAGCTCATTCCCCTGGATTTCCACGACATCCTGCGC
AACCTGGCCCCGCAGGGCCTGGCGCAGTTCGAGGCGTGGACCGCGCGCGAGGCCGCCAGCCAGTCCTAG					