Detailed information of component protein


Summary

Component ID T6CP000019
Component protein type TssM
T6SS ID (Type) T6SS00004 experimental (Type i1)
Strain Pseudomonas aeruginosa PAO1
Replicon chromosome (H2-T6SS)
Sequence Protein sequence (1176 a.a.); Nucleotide sequence (3528 bp)
Reference
[1] Sana TG, Hachani A, Bucior I, et al. The second type VI secretion system of Pseudomonas aeruginosa strain PAO1 is regulated by quorum sensing and Fur and modulates internalization in epithelial cells. J Biol Chem. 2012 Aug 3;287(32):27095-105. doi: 10.1074/jbc.M112.376368. Epub 2012 Jun 4. PMID: 22665491
[2] Osipiuk J, Xu X, Cui H, et al. Crystal structure of secretory protein Hcp3 from Pseudomonas aeruginosa. J Struct Funct Genomics. 2011 Mar;12(1):21-6. doi: 10.1007/s10969-011-9107-1. Epub 2011 Apr 8. PMID: 21476004
[3] Barret M, Egan F, Fargier E, et al. Genomic analysis of the type VI secretion systems in Pseudomonas spp.: novel clusters and putative effectors uncovered. Microbiology (Reading). 2011 Jun;157(Pt 6):1726-1739. doi: 10.1099/mic.0.048645-0. Epub 2011 Apr 7. PMID: 21474537
[4] Hachani A, Lossi NS, Hamilton A, et al. Type VI secretion system in Pseudomonas aeruginosa: secretion and multimerization of VgrG proteins. J Biol Chem. 2011 Apr 8;286(14):12317-27. doi: 10.1074/jbc.M110.193045. Epub 2011 Feb 16. PMID: 21325275
[5] Bleves S, Viarre V, Salacha R, et al. Protein secretion systems in Pseudomonas aeruginosa: A wealth of pathogenic weapons. Int J Med Microbiol. 2010 Dec;300(8):534-43. doi: 10.1016/j.ijmm.2010.08.005. Epub 2010 Oct 13. PMID: 20947426
[6] Russell AB, LeRoux M, Hathazi K, et al. Diverse type VI secretion phospholipases are functionally plastic antibacterial effectors. Nature. 2013 Apr 25;496(7446):508-12. doi: 10.1038/nature12074. Epub 2013 Apr 3. PMID: 23552891
[7] Sana TG, Soscia C, Tonglet CM, et al. Divergent control of two type VI secretion systems by RpoN in Pseudomonas aeruginosa. PLoS One. 2013 Oct 21;8(10):e76030. doi: 10.1371/journal.pone.0076030. eCollection 2013. PMID: 24204589
[8] Jiang F, Waterfield NR, Yang J, et al. A Pseudomonas aeruginosa type VI secretion phospholipase D effector targets both prokaryotic and eukaryotic cells. Cell Host Microbe. 2014 May 14;15(5):600-10. doi: 10.1016/j.chom.2014.04.010. PMID: 24832454
[9] Sana TG, Baumann C, Merdes A, et al. Internalization of Pseudomonas aeruginosa Strain PAO1 into Epithelial Cells Is Promoted by Interaction of a T6SS Effector with the Microtubule Network. mBio. 2015 Jun 2;6(3):e00712. doi: 10.1128/mBio.00712-15. PMID: 26037124
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) PA1669
Coordinate (Strand) 1819074..1822601 (+)
NCBI ID NP_250360.1
RefSeq NC_002516
Uniprot ID Q9I356_PSEAE
KEGG ID pae:PA1669
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
IcmF-related_N PF14331.8 1.2e-78 198..452
IcmF-related PF06761.14 1.3e-71 507..792
IcmF_C PF06744.14 2e-22 1050..1154
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-16 MKNFFKKVGAFLRKTW
Transmembrane helix 17-39 VWTLLLVLLAALLVWFVGPLLAV
Outside 40-48 ADYKFWESP
Transmembrane helix 49-71 TSRLLSISLMFLVWGLAMVFVSW
Inside 72-447 RAGVRRKAVEDSDEGQERLRRDGLIEDEQKELRSRFKDALRTLKTSSLYRGRSERWRNELPWYLLIGPQGSGKTSLLDFSGLEFPINRIDRKLTRDTAGTRYCDWYFADHGVLIDTAGRYLTQADGEVDGGAWATLLGLLRKRRRARPLNGVMVSIPVETLLSDDEMALDTLARQVRARLQEIQQTLHLDLPVYLVLSKADQLLGFDEFFDQLSREESDQVLGTSFGKEQNGTDVTVLRQAFEDLLRRLNSQVIMRIHQERDTLRRGRILDFPHQLGQIGERLCLFVELAFTGNRYQRASRLRGFYLTSAPHLAQQLDEDTAGIGANLGIASGLLPTLRNGRSRFIHHLLSQVIFPEANLAGLDRRERTRIHWGQR
Transmembrane helix 448-470 ALYVGSLALLLLFGLLWAGGFSA
Outside 471-1175 NHERLERLRELAQQWTQQRQALGERDDAMAALKTLDSSYAATTVFPPKGEVSLHERAGLYQGNEVNPPLAAAYRRELETQLLPRVARMLEAQIRGNMQNRERLLNSLRAYLMLNLRERRENGWLKDWVATDWSQRYAGNGTVQNGLNTHFARLLEQPFSYPLNETLVAQARQVLRSESLANVVYRVLREQARSLPEYRFGQHLGPQAGLFVGTDYVIPGFYTQQGYQQYFVVQGASLVNDILRDNWVLGEGSGISDMDLRRLMVELEQLYFRDYANFWSEAVGRLGLLPFNDAGEGADQVSGLLAANSPILQLLLEVRENTRFPVLAESAEALAEASDKAAEKGGKLGKAAAAAAGKARDALAKNLPDTAKKSLQRRFEPLHRLLDDNNGPAADLTPLLQALNELQLQLAGLARASQPEQAAFDMAKSRMGGQRDALSNLRNASARLPRPLGGWFSVLAEDAWRLVLGDAYLYLNQRYQSELYGFYSKAINKRYPFNAHSASDVAVSDFREFFKAQGVAERFFETYMRPFVSGDPGSYRLRSVDGYSLPMSKVYLDQMGTAQLIRQSFFAENPAEPQVRFRLEPYTLDPGVSRAEFRFDGQSMEYRHGPIVPMSFKWPSDADGGRTSLVLEKMAGRPVGIERNTGPWSLFRLFDLMQTEYLSGRDVMVLKADLGGLRANYLLLAQRTPNPFDLAVLRSFRMPVQL


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9955 - -


  Protein sequence: 1176 a.a.    

>T6CP000019 NC_002516:1819074-1822601 [Pseudomonas aeruginosa PAO1] [TssM]
MKNFFKKVGAFLRKTWVWTLLLVLLAALLVWFVGPLLAVADYKFWESPTSRLLSISLMFLVWGLAMVFVSWRAGVRRKAV
EDSDEGQERLRRDGLIEDEQKELRSRFKDALRTLKTSSLYRGRSERWRNELPWYLLIGPQGSGKTSLLDFSGLEFPINRI
DRKLTRDTAGTRYCDWYFADHGVLIDTAGRYLTQADGEVDGGAWATLLGLLRKRRRARPLNGVMVSIPVETLLSDDEMAL
DTLARQVRARLQEIQQTLHLDLPVYLVLSKADQLLGFDEFFDQLSREESDQVLGTSFGKEQNGTDVTVLRQAFEDLLRRL
NSQVIMRIHQERDTLRRGRILDFPHQLGQIGERLCLFVELAFTGNRYQRASRLRGFYLTSAPHLAQQLDEDTAGIGANLG
IASGLLPTLRNGRSRFIHHLLSQVIFPEANLAGLDRRERTRIHWGQRALYVGSLALLLLFGLLWAGGFSANHERLERLRE
LAQQWTQQRQALGERDDAMAALKTLDSSYAATTVFPPKGEVSLHERAGLYQGNEVNPPLAAAYRRELETQLLPRVARMLE
AQIRGNMQNRERLLNSLRAYLMLNLRERRENGWLKDWVATDWSQRYAGNGTVQNGLNTHFARLLEQPFSYPLNETLVAQA
RQVLRSESLANVVYRVLREQARSLPEYRFGQHLGPQAGLFVGTDYVIPGFYTQQGYQQYFVVQGASLVNDILRDNWVLGE
GSGISDMDLRRLMVELEQLYFRDYANFWSEAVGRLGLLPFNDAGEGADQVSGLLAANSPILQLLLEVRENTRFPVLAESA
EALAEASDKAAEKGGKLGKAAAAAAGKARDALAKNLPDTAKKSLQRRFEPLHRLLDDNNGPAADLTPLLQALNELQLQLA
GLARASQPEQAAFDMAKSRMGGQRDALSNLRNASARLPRPLGGWFSVLAEDAWRLVLGDAYLYLNQRYQSELYGFYSKAI
NKRYPFNAHSASDVAVSDFREFFKAQGVAERFFETYMRPFVSGDPGSYRLRSVDGYSLPMSKVYLDQMGTAQLIRQSFFA
ENPAEPQVRFRLEPYTLDPGVSRAEFRFDGQSMEYRHGPIVPMSFKWPSDADGGRTSLVLEKMAGRPVGIERNTGPWSLF
RLFDLMQTEYLSGRDVMVLKADLGGLRANYLLLAQRTPNPFDLAVLRSFRMPVQL*

  Nucleotide sequence: 3528 bp    

>T6CP000019 NC_002516:1819074-1822601 [Pseudomonas aeruginosa PAO1] [TssM]
ATGAAAAACTTCTTCAAGAAAGTCGGCGCCTTCCTGCGCAAGACGTGGGTCTGGACGCTGTTGCTGGTACTCCTGGCGGC
ATTGCTGGTGTGGTTCGTCGGGCCGCTGCTGGCGGTCGCCGACTACAAGTTCTGGGAAAGCCCGACCTCGCGGTTGCTGA
GCATCAGCCTGATGTTCCTGGTCTGGGGGCTGGCGATGGTCTTCGTCAGCTGGCGCGCCGGAGTGCGGCGCAAGGCCGTC
GAGGACAGCGACGAGGGCCAGGAGCGCCTGCGCCGCGACGGTCTGATCGAAGACGAACAGAAGGAATTGCGCAGCCGCTT
CAAGGACGCCTTGCGCACCCTGAAGACCTCCAGCCTGTACCGCGGCCGCAGCGAGCGCTGGCGCAACGAGCTGCCCTGGT
ACCTGCTGATCGGCCCGCAGGGAAGCGGCAAGACCAGCCTGCTGGACTTTTCCGGACTGGAATTCCCGATCAACCGGATC
GACCGCAAGCTGACCCGCGACACCGCCGGCACGCGCTACTGCGACTGGTACTTCGCCGACCACGGCGTCCTGATCGACAC
CGCCGGGCGCTACCTGACCCAAGCCGACGGCGAGGTCGACGGGGGCGCCTGGGCGACCCTGCTCGGCCTGTTGCGCAAGC
GCCGCCGCGCACGGCCGCTGAATGGAGTGATGGTGTCGATTCCGGTGGAAACGTTGCTCAGCGACGACGAGATGGCGCTG
GATACGCTGGCTCGCCAGGTGCGCGCGCGCCTGCAGGAAATCCAGCAGACCCTGCACCTCGATCTGCCGGTCTACCTGGT
GCTGAGCAAGGCCGACCAGTTGCTCGGCTTCGACGAGTTCTTCGACCAGTTGAGCCGCGAGGAGAGCGACCAGGTTCTGG
GCACCAGCTTCGGCAAGGAGCAGAACGGCACCGATGTGACGGTTCTGCGCCAGGCGTTCGAGGACCTTCTGCGACGTCTG
AACAGCCAGGTGATCATGCGCATCCACCAGGAGCGCGATACCCTGCGTCGCGGTCGCATCCTCGACTTCCCGCACCAGCT
TGGCCAGATCGGCGAGCGGTTGTGCCTGTTCGTCGAACTCGCCTTCACTGGCAATCGCTACCAGCGCGCCAGCCGCCTGC
GCGGCTTCTACCTGACCAGCGCGCCGCACCTGGCCCAGCAGCTGGATGAAGACACCGCCGGCATCGGCGCCAACCTGGGG
ATCGCCAGCGGCCTGTTGCCGACCCTGCGCAACGGGCGCTCGCGCTTCATCCATCACCTGCTGAGCCAGGTGATCTTCCC
CGAGGCCAACCTGGCAGGTCTCGATCGCCGCGAACGCACGCGCATCCACTGGGGCCAGCGCGCCCTGTACGTCGGTTCGC
TGGCGCTCCTTCTGCTATTCGGCCTGCTCTGGGCTGGCGGTTTCTCGGCCAACCACGAGCGCCTGGAGCGGCTACGCGAG
CTGGCGCAGCAATGGACCCAGCAGCGCCAGGCGCTTGGCGAGCGCGATGACGCCATGGCGGCGCTGAAGACGCTGGATAG
CAGCTATGCGGCGACCACCGTGTTTCCGCCCAAAGGGGAAGTCTCGCTGCACGAGCGCGCCGGGCTGTACCAGGGCAACG
AGGTCAACCCGCCGCTGGCCGCGGCCTATCGCCGCGAGCTGGAGACGCAACTGCTGCCGCGCGTGGCGCGGATGCTCGAA
GCGCAGATCCGCGGCAACATGCAGAATCGCGAACGCCTGCTCAACAGCCTGCGGGCCTACCTGATGCTGAATCTGCGCGA
GCGCCGCGAGAACGGATGGCTCAAGGACTGGGTGGCTACCGACTGGTCGCAGCGCTATGCCGGCAACGGCACCGTGCAGA
ATGGCCTGAACACGCATTTCGCCCGTCTGCTCGAGCAACCGTTCAGCTATCCGCTGAACGAGACCCTGGTGGCCCAGGCA
CGCCAGGTGCTGCGCAGCGAGTCCCTGGCCAACGTGGTCTACCGGGTGCTGCGCGAACAGGCGCGAAGCCTGCCCGAGTA
TCGCTTCGGCCAGCACCTGGGGCCGCAGGCCGGTCTGTTCGTCGGCACCGACTACGTGATCCCGGGCTTCTACACCCAGC
AGGGCTACCAGCAGTACTTCGTGGTCCAGGGCGCCAGCCTGGTCAACGACATCCTTCGCGACAACTGGGTCCTGGGCGAG
GGCAGCGGGATCAGCGACATGGACCTGCGGCGCCTGATGGTCGAACTCGAGCAGTTGTATTTCCGCGACTACGCGAACTT
CTGGAGCGAGGCGGTCGGACGCCTGGGCCTGCTGCCGTTCAACGATGCCGGCGAGGGCGCCGACCAGGTTTCCGGGCTGC
TCGCCGCCAACTCGCCGATCCTCCAGTTGTTGCTGGAGGTCCGTGAGAACACCCGTTTCCCGGTCCTGGCGGAGAGCGCC
GAGGCGCTGGCAGAGGCCAGCGACAAGGCCGCGGAAAAGGGCGGCAAGCTGGGCAAGGCGGCCGCCGCGGCGGCCGGCAA
GGCCAGGGACGCGTTGGCGAAGAACCTGCCGGATACCGCGAAGAAGTCCCTGCAACGCCGCTTCGAGCCCCTGCATCGCC
TGCTCGACGATAACAACGGCCCGGCGGCCGACCTCACCCCGCTGTTGCAGGCGCTCAACGAGTTGCAGTTGCAACTCGCC
GGACTGGCGCGCGCCAGCCAGCCGGAGCAGGCGGCTTTCGACATGGCGAAGAGCCGCATGGGCGGCCAGCGCGATGCCCT
GAGCAACCTGCGCAACGCCTCCGCGCGACTGCCGCGACCGCTCGGCGGATGGTTCAGCGTGCTGGCCGAAGACGCCTGGC
GGCTGGTCCTCGGCGATGCCTACCTGTATCTCAACCAGCGCTACCAGAGCGAGCTGTACGGTTTCTACAGCAAGGCGATC
AACAAGCGCTACCCGTTCAACGCGCACAGCGCCAGCGATGTCGCGGTCAGCGATTTCCGCGAGTTCTTCAAGGCGCAGGG
CGTCGCCGAACGCTTCTTCGAAACCTACATGCGGCCCTTCGTCAGCGGCGACCCGGGCAGTTACCGCCTGCGCAGCGTGG
ACGGTTACAGCCTGCCGATGTCCAAGGTCTATCTCGACCAGATGGGCACGGCCCAGCTGATCCGCCAGAGCTTCTTCGCC
GAGAACCCGGCCGAGCCACAGGTGCGCTTCCGCCTGGAGCCCTATACCCTCGATCCTGGCGTCAGCCGCGCCGAGTTCCG
TTTCGACGGTCAGTCCATGGAGTACCGCCACGGCCCGATCGTGCCGATGTCGTTCAAGTGGCCCAGCGACGCCGACGGCG
GCCGCACCAGCCTGGTGCTGGAGAAGATGGCCGGACGACCGGTGGGAATCGAACGCAATACCGGGCCCTGGTCGCTGTTC
AGGCTGTTCGACCTGATGCAGACCGAGTACCTCAGCGGGCGTGACGTGATGGTGCTCAAGGCCGACCTGGGCGGCCTGCG
CGCCAACTACCTGCTGCTCGCCCAGCGCACGCCGAATCCGTTCGATCTCGCGGTCCTGCGCAGCTTCCGCATGCCGGTGC
AGCTCTGA