Detailed information of accessory protein


Summary

Accessory protein ID ACP00040 experimental
Type chaperone
Related T6SS (Type) T6SS01070 (Type i4b)
Strain Acinetobacter baumannii strain ATCC 17978
Replicon chromosome
Related structure protein AUO97_14000 (TssM)
Related effector -
Description AsaA located in the periplasm and interacts with TssM
Reference [1] Li L, Wang YN, Jia HB, et al. The type VI secretion system protein AsaA in Acinetobacter baumannii is a periplasmic protein physically interacting with TssM and required for T6SS assembly. Sci Rep. 2019 Jul 1;9(1):9438. doi: 10.1038/s41598-019-45875-9. PMID: 31263148
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed

External database links

Locus tag (Gene) AUO97_13960 (asaA)
Coordinates (Strand) 2936023..2936715 (+)
NCBI ID 446792453
RefSeq NZ_CP018664
Uniprot ID -
KEGG ID -
PDB ID -

Pfam domain hit(s)

Domain Pfam ID E-value Aligned region

Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-230 MKVVLKRSILILFLTGNIIGLSYAQAAELEPVKLKKSCIKKNPLAEGQNDPELLNIFKQVCDSDNTTQKNDLLAKAAMRFYQTNQPVKALNLASQLQSQNIRGSLITDVLFLSGVSIANNSLQQMRNQEMRYLTNDITYPPAKQLVENIHTAMPAPDPSSFKTNNSESSSTDNEKRNYKKQYATRNKVSASRTSNKRVVSPAKATAVKPKTAPAATVNKSKSSPFDPLIK



Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
SP(Sec/SPI) 0.9940 pos: 26-27. AQA-AE. MKVVLKRSILILFLTGNIIGLSYAQA



  Protein sequence: 231 a.a.    

>ACP00040 NZ_CP018664:2936023-2936715 [Acinetobacter baumannii]
MKVVLKRSILILFLTGNIIGLSYAQAAELEPVKLKKSCIKKNPLAEGQNDPELLNIFKQVCDSDNTTQKNDLLAKAAMRF
YQTNQPVKALNLASQLQSQNIRGSLITDVLFLSGVSIANNSLQQMRNQEMRYLTNDITYPPAKQLVENIHTAMPAPDPSS
FKTNNSESSSTDNEKRNYKKQYATRNKVSASRTSNKRVVSPAKATAVKPKTAPAATVNKSKSSPFDPLIK*

  Nucleotide sequence: 693 bp    

>ACP00040 NZ_CP018664:2936023-2936715 [Acinetobacter baumannii]
ATGAAAGTTGTGTTGAAACGAAGTATTTTGATACTTTTTTTGACTGGAAACATTATCGGTCTATCATATGCTCAAGCAGC
AGAACTAGAGCCTGTAAAACTTAAAAAAAGTTGTATTAAGAAGAACCCTCTAGCAGAAGGCCAAAATGACCCTGAGCTAT
TGAATATTTTTAAACAAGTTTGTGATAGTGATAATACAACTCAAAAAAATGATTTATTAGCTAAAGCTGCTATGCGGTTT
TATCAAACCAATCAACCAGTTAAAGCATTGAATTTAGCAAGTCAATTGCAAAGTCAAAATATACGCGGTTCATTAATCAC
AGATGTCTTATTTTTATCTGGTGTGAGCATTGCAAATAACTCATTGCAACAAATGCGTAATCAGGAAATGCGATATTTAA
CGAATGACATAACATATCCTCCTGCAAAGCAGTTAGTTGAAAATATTCATACTGCAATGCCGGCTCCTGATCCATCGAGT
TTCAAAACTAACAACTCGGAAAGCAGTTCTACTGATAATGAAAAAAGAAATTATAAAAAACAGTACGCAACGCGTAATAA
AGTGAGTGCTTCTAGAACATCAAATAAACGAGTAGTAAGTCCAGCAAAGGCCACCGCTGTCAAACCTAAAACTGCACCTG
CAGCCACTGTCAATAAATCAAAAAGTTCGCCTTTTGACCCTTTAATTAAATAA