Detailed information of accessory protein
Summary
| Accessory protein ID | ACP00040  |
| Type | chaperone |
| Related T6SS (Type) | T6SS01070 (Type i4b) |
| Strain | Acinetobacter baumannii strain ATCC 17978 |
| Replicon | chromosome |
| Related structure protein | AUO97_14000 (TssM) |
| Related effector | - |
| Description | AsaA located in the periplasm and interacts with TssM |
| Reference | [1] Li L, Wang YN, Jia HB, et al. The type VI secretion system protein AsaA in Acinetobacter baumannii is a periplasmic protein physically interacting with TssM and required for T6SS assembly. Sci Rep. 2019 Jul 1;9(1):9438. doi: 10.1038/s41598-019-45875-9. PMID: 31263148 |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | AUO97_13960 (asaA) |
| Coordinates (Strand) | 2936023..2936715 (+) |
| NCBI ID | 446792453 |
| RefSeq | NZ_CP018664 |
| Uniprot ID | - |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-230 | MKVVLKRSILILFLTGNIIGLSYAQAAELEPVKLKKSCIKKNPLAEGQNDPELLNIFKQVCDSDNTTQKNDLLAKAAMRFYQTNQPVKALNLASQLQSQNIRGSLITDVLFLSGVSIANNSLQQMRNQEMRYLTNDITYPPAKQLVENIHTAMPAPDPSSFKTNNSESSSTDNEKRNYKKQYATRNKVSASRTSNKRVVSPAKATAVKPKTAPAATVNKSKSSPFDPLIK |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| SP(Sec/SPI) | 0.9940 | pos: 26-27. AQA-AE. | MKVVLKRSILILFLTGNIIGLSYAQA |
Protein sequence: 231 a.a.
>ACP00040 NZ_CP018664:2936023-2936715 [Acinetobacter baumannii]
MKVVLKRSILILFLTGNIIGLSYAQAAELEPVKLKKSCIKKNPLAEGQNDPELLNIFKQVCDSDNTTQKNDLLAKAAMRF
YQTNQPVKALNLASQLQSQNIRGSLITDVLFLSGVSIANNSLQQMRNQEMRYLTNDITYPPAKQLVENIHTAMPAPDPSS
FKTNNSESSSTDNEKRNYKKQYATRNKVSASRTSNKRVVSPAKATAVKPKTAPAATVNKSKSSPFDPLIK*
MKVVLKRSILILFLTGNIIGLSYAQAAELEPVKLKKSCIKKNPLAEGQNDPELLNIFKQVCDSDNTTQKNDLLAKAAMRF
YQTNQPVKALNLASQLQSQNIRGSLITDVLFLSGVSIANNSLQQMRNQEMRYLTNDITYPPAKQLVENIHTAMPAPDPSS
FKTNNSESSSTDNEKRNYKKQYATRNKVSASRTSNKRVVSPAKATAVKPKTAPAATVNKSKSSPFDPLIK*
Nucleotide sequence: 693 bp
>ACP00040 NZ_CP018664:2936023-2936715 [Acinetobacter baumannii]
ATGAAAGTTGTGTTGAAACGAAGTATTTTGATACTTTTTTTGACTGGAAACATTATCGGTCTATCATATGCTCAAGCAGC
AGAACTAGAGCCTGTAAAACTTAAAAAAAGTTGTATTAAGAAGAACCCTCTAGCAGAAGGCCAAAATGACCCTGAGCTAT
TGAATATTTTTAAACAAGTTTGTGATAGTGATAATACAACTCAAAAAAATGATTTATTAGCTAAAGCTGCTATGCGGTTT
TATCAAACCAATCAACCAGTTAAAGCATTGAATTTAGCAAGTCAATTGCAAAGTCAAAATATACGCGGTTCATTAATCAC
AGATGTCTTATTTTTATCTGGTGTGAGCATTGCAAATAACTCATTGCAACAAATGCGTAATCAGGAAATGCGATATTTAA
CGAATGACATAACATATCCTCCTGCAAAGCAGTTAGTTGAAAATATTCATACTGCAATGCCGGCTCCTGATCCATCGAGT
TTCAAAACTAACAACTCGGAAAGCAGTTCTACTGATAATGAAAAAAGAAATTATAAAAAACAGTACGCAACGCGTAATAA
AGTGAGTGCTTCTAGAACATCAAATAAACGAGTAGTAAGTCCAGCAAAGGCCACCGCTGTCAAACCTAAAACTGCACCTG
CAGCCACTGTCAATAAATCAAAAAGTTCGCCTTTTGACCCTTTAATTAAATAA
ATGAAAGTTGTGTTGAAACGAAGTATTTTGATACTTTTTTTGACTGGAAACATTATCGGTCTATCATATGCTCAAGCAGC
AGAACTAGAGCCTGTAAAACTTAAAAAAAGTTGTATTAAGAAGAACCCTCTAGCAGAAGGCCAAAATGACCCTGAGCTAT
TGAATATTTTTAAACAAGTTTGTGATAGTGATAATACAACTCAAAAAAATGATTTATTAGCTAAAGCTGCTATGCGGTTT
TATCAAACCAATCAACCAGTTAAAGCATTGAATTTAGCAAGTCAATTGCAAAGTCAAAATATACGCGGTTCATTAATCAC
AGATGTCTTATTTTTATCTGGTGTGAGCATTGCAAATAACTCATTGCAACAAATGCGTAATCAGGAAATGCGATATTTAA
CGAATGACATAACATATCCTCCTGCAAAGCAGTTAGTTGAAAATATTCATACTGCAATGCCGGCTCCTGATCCATCGAGT
TTCAAAACTAACAACTCGGAAAGCAGTTCTACTGATAATGAAAAAAGAAATTATAAAAAACAGTACGCAACGCGTAATAA
AGTGAGTGCTTCTAGAACATCAAATAAACGAGTAGTAAGTCCAGCAAAGGCCACCGCTGTCAAACCTAAAACTGCACCTG
CAGCCACTGTCAATAAATCAAAAAGTTCGCCTTTTGACCCTTTAATTAAATAA