Detailed information of accessory protein


Summary

Accessory protein ID ACP00039 experimental
Type structure affiliated protein
Related T6SS (Type) T6SS01046 (Type i4b)
Strain Acinetobacter baumannii strain ATCC 17978
Replicon chromosome
Related structure protein -
Related effector peptidoglycan
Description TagX fulfills the important role of enabling the transit of T6SS machinery across the peptidoglycan layer of the T6SS-producing bacterium
Reference [1] Weber BS, Hennon SW, Wright MS, et al. Genetic Dissection of the Type VI Secretion System in Acinetobacter and Identification of a Novel Peptidoglycan Hydrolase, TagX, Required for Its Biogenesis. mBio. 2016 Oct 11;7(5). pii: e01253-16. doi: 10.1128/mBio.01253-16. PMID: 27729508
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed
External database links

Locus tag (Gene) ACX60_11600 (tagX)
Coordinates (Strand) 2432052..2433005 (-)
NCBI ID 446895327
RefSeq NZ_CP012004
Uniprot ID A0A0E1PUV5_ACIBA, A0A2S4T371_9GAMM, B0VB12_ACIBY, D0C8M9_ACIB2
KEGG ID aby:ABAYE2399
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
Peptidase_M15_4 PF13539.8 7.7e-15 227..293
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-3 MMI
Transmembrane helix 4-23 FLIFFCFACLAIGLAFLMSY
Inside 24-71 ELRNKGRTFVLSLLPQGRRQLNQVRQFAQTMNQAAAPEKLQSHWHLQQ
Transmembrane helix 72-94 WWIVIAGFFLFASILIFAFTRPI
Outside 95-317 SSTRIEAEYLKKTDPQIYALLNGEILSPPPEVDESLIEAAIVEATQLEQQYASQESGVINSSPIDNTSFDGRAILDTSLVNRKWDKMNPRYKQRLLMVFKIMKEQYGYELVLLEGYRSPARQNMLAGNPNTTRAKGYQSYHQFGLAADVAFKRNGKVVISERDPWAMQGYRLYGQVAESVGLTWGGRWKSIQDYGHTEYRMPGLKKTQEMAEKLIAESLNDIS


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.8889 - -


  Protein sequence: 318 a.a.    

>ACP00039 NZ_CP012004:c2433005-2432052 [Acinetobacter baumannii]
MMIFLIFFCFACLAIGLAFLMSYELRNKGRTFVLSLLPQGRRQLNQVRQFAQTMNQAAAPEKLQSHWHLQQWWIVIAGFF
LFASILIFAFTRPISSTRIEAEYLKKTDPQIYALLNGEILSPPPEVDESLIEAAIVEATQLEQQYASQESGVINSSPIDN
TSFDGRAILDTSLVNRKWDKMNPRYKQRLLMVFKIMKEQYGYELVLLEGYRSPARQNMLAGNPNTTRAKGYQSYHQFGLA
ADVAFKRNGKVVISERDPWAMQGYRLYGQVAESVGLTWGGRWKSIQDYGHTEYRMPGLKKTQEMAEKLIAESLNDIS*

  Nucleotide sequence: 954 bp    

>ACP00039 NZ_CP012004:c2433005-2432052 [Acinetobacter baumannii]
ATGATGATTTTTCTAATTTTCTTTTGTTTTGCTTGTCTTGCTATAGGGCTAGCATTTTTGATGTCTTATGAGTTAAGAAA
TAAGGGTAGAACATTTGTTCTATCTTTATTACCTCAGGGGCGTCGGCAGCTAAACCAAGTTAGACAATTTGCTCAAACAA
TGAATCAGGCTGCGGCTCCTGAAAAACTACAATCACATTGGCATTTACAGCAATGGTGGATTGTAATTGCAGGATTTTTT
TTATTTGCCAGTATTTTAATTTTTGCTTTTACTCGCCCAATCAGTTCAACACGAATTGAAGCCGAGTATCTTAAAAAAAC
TGATCCGCAAATTTATGCATTATTAAATGGAGAAATATTATCGCCTCCACCTGAAGTAGATGAAAGCCTGATTGAAGCTG
CAATTGTTGAAGCAACTCAGTTAGAGCAGCAATATGCTTCTCAAGAAAGTGGTGTTATAAATTCTTCACCAATTGATAAT
ACAAGTTTTGATGGCCGTGCGATTTTAGATACGAGTTTAGTCAACCGTAAGTGGGATAAAATGAACCCACGCTATAAGCA
GCGTTTGCTTATGGTCTTTAAAATTATGAAAGAACAATATGGTTATGAACTTGTTTTGCTTGAAGGTTATCGTAGTCCGG
CACGTCAAAATATGTTGGCAGGCAATCCGAATACAACCAGAGCAAAAGGGTATCAAAGTTATCATCAATTTGGTTTAGCG
GCAGATGTGGCCTTTAAGCGAAACGGTAAAGTTGTTATTTCTGAACGTGACCCATGGGCTATGCAAGGTTACCGCCTCTA
TGGGCAAGTTGCTGAGTCCGTCGGGCTTACCTGGGGTGGTCGCTGGAAATCTATACAAGATTATGGCCATACAGAATATA
GAATGCCAGGCTTGAAGAAAACTCAAGAAATGGCGGAAAAGCTGATTGCCGAAAGCTTAAATGACATCAGTTAA