Detailed information of accessory protein
Summary
| Accessory protein ID | ACP00019  |
| Type | structure affiliated protein |
| Related T6SS (Type) | T6SS00080 (Type ii) |
| Strain | Francisella tularensis subsp. tularensis SCHU S4 |
| Replicon | chromosome |
| Related structure protein | FTT_1713c (IglB2); FTT_1712c (IglC2) |
| Related effector | - |
| Description | the T6SS of Francisella requires the FipB protein for proper conformation of IglB and IglC |
| Reference | [1] Qin A, Zhang Y, Clark ME, et al. Components of the type six secretion system are substrates of Francisella tularensis Schu S4 DsbA-like FipB protein. Virulence. 2016 Nov 16;7(8):882-894. Epub 2016 Mar 30. PMID: 27028889 |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | FTT_0682c (fipB/dsbA) |
| Coordinates (Strand) | 699171..700016 (-) |
| NCBI ID | 56707802 |
| RefSeq | NC_006570 |
| Uniprot ID | Q5NGZ4_FRATT |
| KEGG ID | ftu:FTT_0682c |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Inside | 1-6 | MQKKSF |
| Transmembrane helix | 7-26 | SILVLFVVILLVVLYIFSTT |
| Outside | 27-281 | NLNKISPDNKQGLYLVEHAFPQYKVIKTFDTGIHLQAYILEDKKDPTKHSVTFTSEDGAVIVNGELLAWDSNQNKLTSLNNIYANYFTSSPQANNLYLNIKKYATYIQQGSDDAPHKFYAIINPSCSYCNRLFDATQPAINSGELAVRWIPLGALRNSPEIVRSIFNSKDPLEALIKYHKTKTYDQKLTQQNEKAENNLRLSSDIEGFPTIVYKTPQGALKISGGNKLPLTDATIAAKDNINKINEFLLLTSNSF |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9269 | - | - |
Protein sequence: 282 a.a.
>ACP00019 NC_006570:c700016-699171 [Francisella tularensis subsp. tularensis SCHU S4]
MQKKSFSILVLFVVILLVVLYIFSTTNLNKISPDNKQGLYLVEHAFPQYKVIKTFDTGIHLQAYILEDKKDPTKHSVTFT
SEDGAVIVNGELLAWDSNQNKLTSLNNIYANYFTSSPQANNLYLNIKKYATYIQQGSDDAPHKFYAIINPSCSYCNRLFD
ATQPAINSGELAVRWIPLGALRNSPEIVRSIFNSKDPLEALIKYHKTKTYDQKLTQQNEKAENNLRLSSDIEGFPTIVYK
TPQGALKISGGNKLPLTDATIAAKDNINKINEFLLLTSNSF*
MQKKSFSILVLFVVILLVVLYIFSTTNLNKISPDNKQGLYLVEHAFPQYKVIKTFDTGIHLQAYILEDKKDPTKHSVTFT
SEDGAVIVNGELLAWDSNQNKLTSLNNIYANYFTSSPQANNLYLNIKKYATYIQQGSDDAPHKFYAIINPSCSYCNRLFD
ATQPAINSGELAVRWIPLGALRNSPEIVRSIFNSKDPLEALIKYHKTKTYDQKLTQQNEKAENNLRLSSDIEGFPTIVYK
TPQGALKISGGNKLPLTDATIAAKDNINKINEFLLLTSNSF*
Nucleotide sequence: 846 bp
>ACP00019 NC_006570:c700016-699171 [Francisella tularensis subsp. tularensis SCHU S4]
ATGCAAAAAAAAAGCTTCTCTATTCTAGTTTTATTTGTTGTTATACTATTAGTTGTACTGTATATTTTTAGTACTACTAA
TCTTAATAAAATCTCACCAGATAACAAACAAGGCTTATACCTTGTTGAGCATGCCTTTCCACAATATAAGGTTATAAAGA
CTTTTGACACAGGTATCCATCTACAAGCATATATTCTAGAAGACAAAAAAGATCCAACAAAACATAGTGTTACTTTTACT
AGTGAAGATGGTGCTGTAATTGTTAATGGTGAACTTTTAGCATGGGATAGTAATCAAAACAAGCTAACTAGTTTAAATAA
TATTTATGCAAACTATTTCACTTCATCACCTCAAGCGAACAATCTTTATCTAAACATCAAAAAGTATGCTACATACATCC
AGCAAGGCAGTGATGATGCGCCACATAAGTTCTATGCTATTATAAACCCTAGTTGCAGCTATTGTAATCGTCTATTTGAT
GCGACTCAACCAGCAATTAACTCAGGAGAACTTGCAGTACGCTGGATTCCTTTAGGTGCACTGCGTAATAGCCCAGAGAT
TGTTAGAAGTATTTTTAACTCAAAAGATCCTCTTGAAGCATTGATTAAATATCATAAGACAAAAACATATGATCAGAAAT
TAACTCAACAAAATGAAAAAGCCGAAAATAATTTAAGGTTATCGAGTGATATTGAAGGTTTCCCTACAATTGTTTATAAA
ACACCTCAAGGAGCTTTAAAAATCTCAGGTGGTAACAAGCTTCCACTTACAGATGCTACTATAGCAGCAAAAGATAATAT
CAATAAAATTAATGAATTTTTGCTATTAACCTCAAATAGTTTCTAA
ATGCAAAAAAAAAGCTTCTCTATTCTAGTTTTATTTGTTGTTATACTATTAGTTGTACTGTATATTTTTAGTACTACTAA
TCTTAATAAAATCTCACCAGATAACAAACAAGGCTTATACCTTGTTGAGCATGCCTTTCCACAATATAAGGTTATAAAGA
CTTTTGACACAGGTATCCATCTACAAGCATATATTCTAGAAGACAAAAAAGATCCAACAAAACATAGTGTTACTTTTACT
AGTGAAGATGGTGCTGTAATTGTTAATGGTGAACTTTTAGCATGGGATAGTAATCAAAACAAGCTAACTAGTTTAAATAA
TATTTATGCAAACTATTTCACTTCATCACCTCAAGCGAACAATCTTTATCTAAACATCAAAAAGTATGCTACATACATCC
AGCAAGGCAGTGATGATGCGCCACATAAGTTCTATGCTATTATAAACCCTAGTTGCAGCTATTGTAATCGTCTATTTGAT
GCGACTCAACCAGCAATTAACTCAGGAGAACTTGCAGTACGCTGGATTCCTTTAGGTGCACTGCGTAATAGCCCAGAGAT
TGTTAGAAGTATTTTTAACTCAAAAGATCCTCTTGAAGCATTGATTAAATATCATAAGACAAAAACATATGATCAGAAAT
TAACTCAACAAAATGAAAAAGCCGAAAATAATTTAAGGTTATCGAGTGATATTGAAGGTTTCCCTACAATTGTTTATAAA
ACACCTCAAGGAGCTTTAAAAATCTCAGGTGGTAACAAGCTTCCACTTACAGATGCTACTATAGCAGCAAAAGATAATAT
CAATAAAATTAATGAATTTTTGCTATTAACCTCAAATAGTTTCTAA