Detailed information of accessory protein


Summary

Accessory protein ID ACP00017 experimental
Type chaperone
Related T6SS (Type) T6SS00061 (Type i4b)
Strain Acinetobacter baylyi ADP1
Replicon chromosome
Related structure protein -
Related effector -
Description Strongly reduced frequency of T6SS assembly observed by fluorescence microscopy
Reference [1] Ringel PD, Hu D, Basler M. The Role of Type VI Secretion System Effectors in Target Cell Lysis and Subsequent Horizontal Gene Transfer. Cell Rep. 2017 Dec 26;21(13):3927-3940. doi: 10.1016/j.celrep.2017.12.020. PMID: 29281838
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed
External database links

Locus tag (Gene) ACIAD_RS12210
Coordinates (Strand) 2641820..2643223 (-)
NCBI ID WP_004928997.1
RefSeq NC_005966
Uniprot ID UPI0002CFCCC9
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-4 MIKK
Transmembrane helix 5-24 IISVLVLVLVVVTSVMIVYW
Outside 25-35 KDSQFDPSAQD
Transmembrane helix 36-58 IWLYLVILPLVLGSVLLAPWLLF
Inside 59-467 KAYRAYRQRQQELAIQKEITPEKSEIASETTPEWITLNVFSSYAFATAGENNELLDAIMDYQSPQLDTELVNAYGLPILSYRITDLTLEQNETEYSARQQRIRALITQQIQQQHMTLLNIAEHLKHSSLFYETEHVQAYRMHPAWINEQYTDQEEDSSQTAPEPVSQLTRLNVHIFLPEDLLHFWNDDENNDFLNQQLIQLGFIPEKIFIEYQFVSAVSAYTEWNELLKRIAIKTDEVTLVVSVDSEIDQELIEERFWQHQNYIPSEFIASCCLAAESVLINSLQPLKQLKLCTNHSDTALALNHLQLHDLPQYQDEQPFVFILEQAADFGMSKSLDQHFNRTSIEPHHYLYACSLIGHTQQVSKIYGAMLALQTIDQSVAFIFSRHALSTFTVVQNAISEIEQEEEAA


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9933 - -


  Protein sequence: 468 a.a.    

>ACP00017 NC_005966:c2643223-2641820 [Acinetobacter baylyi ADP1]
MIKKIISVLVLVLVVVTSVMIVYWKDSQFDPSAQDIWLYLVILPLVLGSVLLAPWLLFKAYRAYRQRQQELAIQKEITPE
KSEIASETTPEWITLNVFSSYAFATAGENNELLDAIMDYQSPQLDTELVNAYGLPILSYRITDLTLEQNETEYSARQQRI
RALITQQIQQQHMTLLNIAEHLKHSSLFYETEHVQAYRMHPAWINEQYTDQEEDSSQTAPEPVSQLTRLNVHIFLPEDLL
HFWNDDENNDFLNQQLIQLGFIPEKIFIEYQFVSAVSAYTEWNELLKRIAIKTDEVTLVVSVDSEIDQELIEERFWQHQN
YIPSEFIASCCLAAESVLINSLQPLKQLKLCTNHSDTALALNHLQLHDLPQYQDEQPFVFILEQAADFGMSKSLDQHFNR
TSIEPHHYLYACSLIGHTQQVSKIYGAMLALQTIDQSVAFIFSRHALSTFTVVQNAISEIEQEEEAA*

  Nucleotide sequence: 1404 bp    

>ACP00017 NC_005966:c2643223-2641820 [Acinetobacter baylyi ADP1]
ATGATTAAAAAGATTATATCTGTTCTGGTACTGGTTTTGGTAGTGGTCACCAGTGTCATGATTGTGTATTGGAAAGATAG
CCAGTTTGATCCAAGTGCTCAAGATATCTGGCTTTATTTGGTGATACTACCACTGGTTCTTGGCAGTGTTTTACTGGCAC
CATGGTTGTTGTTTAAAGCATATCGTGCTTATCGGCAACGTCAGCAAGAGCTTGCTATACAAAAAGAAATAACGCCTGAA
AAGTCTGAAATAGCATCTGAAACTACGCCTGAGTGGATTACACTTAACGTTTTTTCGAGTTATGCCTTTGCAACTGCTGG
AGAGAATAACGAGCTTCTGGACGCAATAATGGACTATCAAAGTCCACAGTTGGATACCGAACTGGTCAACGCATATGGTC
TTCCAATCTTGTCATATCGGATTACAGATTTAACCCTAGAACAAAACGAAACCGAGTATTCAGCGCGTCAGCAACGAATC
CGTGCGTTGATAACTCAACAAATCCAACAACAACACATGACCTTATTAAACATTGCAGAGCATTTAAAGCATTCTAGCTT
GTTTTATGAGACAGAACATGTACAGGCTTATCGGATGCATCCCGCCTGGATCAATGAGCAATATACTGATCAAGAAGAAG
ATTCCTCTCAAACTGCTCCAGAACCAGTTTCACAACTAACACGTTTGAATGTACACATTTTTTTACCAGAAGATTTGCTG
CATTTTTGGAATGATGATGAAAATAATGATTTCCTAAATCAGCAACTTATACAGCTAGGTTTTATTCCAGAAAAAATATT
CATTGAATATCAATTTGTCAGTGCTGTGTCTGCTTATACTGAATGGAACGAGCTATTAAAGCGTATTGCGATAAAAACAG
ATGAAGTGACTTTAGTTGTTTCAGTCGATTCTGAAATTGATCAGGAGCTGATTGAAGAACGATTCTGGCAACATCAGAAT
TATATTCCATCAGAATTTATTGCCAGTTGTTGTCTAGCTGCTGAATCAGTATTGATAAATTCACTGCAACCCTTAAAACA
GTTAAAACTCTGCACCAATCATTCAGATACTGCTTTGGCATTAAACCATCTGCAATTGCATGATTTGCCACAATATCAAG
ATGAGCAGCCTTTTGTTTTTATTCTTGAACAAGCGGCCGATTTCGGGATGTCGAAATCACTTGATCAGCATTTTAATCGA
ACCTCCATCGAGCCACATCATTATCTATATGCATGTTCGCTAATTGGACATACTCAGCAGGTCAGTAAAATTTATGGTGC
AATGCTGGCTTTGCAAACGATAGATCAATCTGTTGCATTTATTTTTAGTCGTCACGCGCTTTCGACATTCACTGTTGTTC
AGAATGCAATCTCGGAAATAGAGCAAGAAGAAGAGGCAGCATGA