Detailed information of accessory protein
Summary
| Accessory protein ID | ACP00013  |
| Type | adaptor |
| Related T6SS (Type) | T6SS00035 (Type i3) |
| Strain | Pseudomonas protegens Pf-5 |
| Replicon | chromosome |
| Related structure protein | - |
| Related effector | PFL_6209 (Tne2) |
| Description | A ΔPFL_6099 strain outcompeted ΔrhsA ΔrhsI ΔpppA but not Δtne2 Δtni2 ΔpppA recipients, and Tne2 accumulated in cells containing PFL_6099, Tne2 was absent in a strain lacking PFL_6099 |
| Reference | [1] Ahmad S, Tsang KK, Sachar K, et al. Structural basis for effector transmembrane domain recognition by type VI secretion system chaperones. Elife. 2020 Dec 15;9. pii: e62816. doi: 10.7554/eLife.62816. PMID: 33320089 |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | PFL_6099 (eagT2) |
| Coordinates (Strand) | 6930853..6931287 (-) |
| NCBI ID | 499376689 |
| RefSeq | NC_004129 |
| Uniprot ID | A0A1K2A9V9_9PSED, A0A2T0Z787_9PSED, Q4K3M6_PSEF5 |
| KEGG ID | pfl:PFL_6099 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| DcrB | PF08786.13 | 6.7e-31 | 8..136 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-144 | MSTYRIQEADLDIPDAWQDQSINIFKLPAVGGAQEASFVISRDPSQGDAAFADYVAAQLNAAEQQLPGFNLIKRWDFDMQGHAAVLLDYSWKREGRELMLRQVFIERKPAVLITTLTTTVNDLVHHEPAWKQAMQSLKPQPSKA |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9855 | - | - |
Protein sequence: 145 a.a.
>ACP00013 NC_004129:c6931287-6930853 [Pseudomonas protegens Pf-5]
MSTYRIQEADLDIPDAWQDQSINIFKLPAVGGAQEASFVISRDPSQGDAAFADYVAAQLNAAEQQLPGFNLIKRWDFDMQ
GHAAVLLDYSWKREGRELMLRQVFIERKPAVLITTLTTTVNDLVHHEPAWKQAMQSLKPQPSKA*
MSTYRIQEADLDIPDAWQDQSINIFKLPAVGGAQEASFVISRDPSQGDAAFADYVAAQLNAAEQQLPGFNLIKRWDFDMQ
GHAAVLLDYSWKREGRELMLRQVFIERKPAVLITTLTTTVNDLVHHEPAWKQAMQSLKPQPSKA*
Nucleotide sequence: 435 bp
>ACP00013 NC_004129:c6931287-6930853 [Pseudomonas protegens Pf-5]
TTGTCCACCTACCGCATTCAAGAAGCCGATCTCGATATTCCTGACGCCTGGCAGGACCAGAGCATCAATATCTTCAAGCT
GCCAGCCGTTGGCGGTGCCCAGGAAGCCAGCTTCGTCATCAGCCGCGACCCGAGCCAGGGCGACGCCGCCTTTGCCGATT
ATGTCGCCGCGCAGCTGAATGCTGCCGAGCAGCAACTGCCAGGTTTCAACCTGATCAAGCGCTGGGACTTCGACATGCAG
GGCCATGCCGCCGTGCTGCTGGACTACAGCTGGAAACGCGAAGGGCGCGAGCTGATGCTGCGCCAGGTCTTCATCGAGCG
TAAGCCGGCCGTGCTGATCACCACCCTGACCACCACGGTCAACGACCTGGTGCATCATGAGCCGGCCTGGAAACAGGCCA
TGCAGAGCCTCAAGCCGCAGCCTTCGAAAGCCTGA
TTGTCCACCTACCGCATTCAAGAAGCCGATCTCGATATTCCTGACGCCTGGCAGGACCAGAGCATCAATATCTTCAAGCT
GCCAGCCGTTGGCGGTGCCCAGGAAGCCAGCTTCGTCATCAGCCGCGACCCGAGCCAGGGCGACGCCGCCTTTGCCGATT
ATGTCGCCGCGCAGCTGAATGCTGCCGAGCAGCAACTGCCAGGTTTCAACCTGATCAAGCGCTGGGACTTCGACATGCAG
GGCCATGCCGCCGTGCTGCTGGACTACAGCTGGAAACGCGAAGGGCGCGAGCTGATGCTGCGCCAGGTCTTCATCGAGCG
TAAGCCGGCCGTGCTGATCACCACCCTGACCACCACGGTCAACGACCTGGTGCATCATGAGCCGGCCTGGAAACAGGCCA
TGCAGAGCCTCAAGCCGCAGCCTTCGAAAGCCTGA