Detailed information of accessory protein
Summary
| Accessory protein ID | ACP00012  |
| Type | adaptor |
| Related T6SS (Type) | T6SS00035 (Type i3) |
| Strain | Pseudomonas protegens Pf-5 |
| Replicon | chromosome |
| Related structure protein | - |
| Related effector | PFL_6096 (RhsA) |
| Description | EagR1 stabilizes the N-terminus of RhsA, which may also orient the effector such that it can interact with its cognate VgrG |
| Reference | [1] Ahmad S, Tsang KK, Sachar K, et al. Structural basis for effector transmembrane domain recognition by type VI secretion system chaperones. Elife. 2020 Dec 15;9. pii: e62816. doi: 10.7554/eLife.62816. PMID: 33320089 |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | PFL_6095 (eagR1) |
| Coordinates (Strand) | 6924978..6925403 (+) |
| NCBI ID | 499376685 |
| RefSeq | NC_004129 |
| Uniprot ID | A0A2C9EVT3_PSEPH, A0A1K2A9S4_9PSED, A0A2T0Z7R4_9PSED, Q4K3N0_PSEF5 |
| KEGG ID | pprc:PFLCHA0_c60540, pfl:PFL_6095 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| DcrB | PF08786.13 | 8.8e-28 | 7..137 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-141 | MTYRLNELQFQLPAGELQDASINILKFPALGTSLIVSRSLLGDGETLESNLASQLQRLEQQVQDLRCQAPQPVQLGPAQDIQGLELRSQFNKGKEQVFQFQLALVLPGTRQMLAMSYVKAQKLGDAEAAHWAQIKNTLVLG |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9541 | - | - |
Protein sequence: 142 a.a.
>ACP00012 NC_004129:6924978-6925403 [Pseudomonas protegens Pf-5]
MTYRLNELQFQLPAGELQDASINILKFPALGTSLIVSRSLLGDGETLESNLASQLQRLEQQVQDLRCQAPQPVQLGPAQD
IQGLELRSQFNKGKEQVFQFQLALVLPGTRQMLAMSYVKAQKLGDAEAAHWAQIKNTLVLG*
MTYRLNELQFQLPAGELQDASINILKFPALGTSLIVSRSLLGDGETLESNLASQLQRLEQQVQDLRCQAPQPVQLGPAQD
IQGLELRSQFNKGKEQVFQFQLALVLPGTRQMLAMSYVKAQKLGDAEAAHWAQIKNTLVLG*
Nucleotide sequence: 426 bp
>ACP00012 NC_004129:6924978-6925403 [Pseudomonas protegens Pf-5]
ATGACCTATCGCCTCAATGAATTGCAGTTCCAACTGCCTGCCGGCGAACTGCAGGACGCCTCGATCAACATCCTCAAGTT
CCCGGCCTTGGGCACTTCGCTGATCGTCAGCCGCAGCCTGCTGGGGGACGGCGAAACCCTGGAGAGCAACCTGGCCAGCC
AGCTGCAGCGGCTGGAGCAGCAAGTCCAGGACCTGCGCTGCCAGGCTCCGCAACCGGTGCAGCTGGGCCCGGCCCAGGAC
ATCCAGGGCCTGGAGCTGCGCAGCCAGTTCAACAAGGGCAAAGAACAGGTGTTCCAGTTCCAGCTGGCCCTGGTGCTGCC
CGGCACCCGACAGATGCTCGCCATGAGCTACGTCAAGGCACAGAAGCTCGGCGACGCGGAAGCCGCCCATTGGGCGCAGA
TCAAGAACACCCTGGTGCTGGGCTGA
ATGACCTATCGCCTCAATGAATTGCAGTTCCAACTGCCTGCCGGCGAACTGCAGGACGCCTCGATCAACATCCTCAAGTT
CCCGGCCTTGGGCACTTCGCTGATCGTCAGCCGCAGCCTGCTGGGGGACGGCGAAACCCTGGAGAGCAACCTGGCCAGCC
AGCTGCAGCGGCTGGAGCAGCAAGTCCAGGACCTGCGCTGCCAGGCTCCGCAACCGGTGCAGCTGGGCCCGGCCCAGGAC
ATCCAGGGCCTGGAGCTGCGCAGCCAGTTCAACAAGGGCAAAGAACAGGTGTTCCAGTTCCAGCTGGCCCTGGTGCTGCC
CGGCACCCGACAGATGCTCGCCATGAGCTACGTCAAGGCACAGAAGCTCGGCGACGCGGAAGCCGCCCATTGGGCGCAGA
TCAAGAACACCCTGGTGCTGGGCTGA