Detailed information of accessory protein
Summary
| Accessory protein ID | ACP00009  |
| Type | structure affiliated protein |
| Related T6SS (Type) | T6SS00014 (Type i4b) |
| Strain | Pseudomonas putida KT2440 |
| Replicon | chromosome |
| Related structure protein | PP_RS15990 (TssA) |
| Related effector | - |
| Description | TagB1 plays a stabilizing role in as- sembly of the T6SS sheath |
| Reference | [1] Bernal P, Furniss RCD, Fecht S, et al. A novel stabilization mechanism for the type VI secretion system sheath. Proc Natl Acad Sci U S A. 2021 Feb 16;118(7). pii: e2008500118. doi: 10.1073/pnas.2008500118. PMID: 33558227 |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | PP_RS16060 (tagB1) |
| Coordinates (Strand) | 3496832..3497581 (-) |
| NCBI ID | 898330878 |
| RefSeq | NC_002947 |
| Uniprot ID | A0A2V4G1L0_9PSED |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-249 | MTQTFDVEALIKLRSQTRAISDALKAQAADYLATVAPLIRPQTLFGEYLQGAQRSSGRETQGHFQALVELYERIGSAAPFQLVTELEVPLNLISTTPELFPLEYDKTLEHSGQVIRITSPTRWVVGFHAFDLARFRNVIKDPNRSSAELYRFVVHYLVLFYCLSKSPGLGRLFEGLRYGLSFERLKGFGDLPFCVISSPVRSELPDDSVIRSSTQIAGNTSFEELVGRDNILEMNGEIRQRLLLTIEGL |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9861 | - | - |
Protein sequence: 250 a.a.
>ACP00009 NC_002947:c3497581-3496832 [Pseudomonas putida KT2440]
MTQTFDVEALIKLRSQTRAISDALKAQAADYLATVAPLIRPQTLFGEYLQGAQRSSGRETQGHFQALVELYERIGSAAPF
QLVTELEVPLNLISTTPELFPLEYDKTLEHSGQVIRITSPTRWVVGFHAFDLARFRNVIKDPNRSSAELYRFVVHYLVLF
YCLSKSPGLGRLFEGLRYGLSFERLKGFGDLPFCVISSPVRSELPDDSVIRSSTQIAGNTSFEELVGRDNILEMNGEIRQ
RLLLTIEGL*
MTQTFDVEALIKLRSQTRAISDALKAQAADYLATVAPLIRPQTLFGEYLQGAQRSSGRETQGHFQALVELYERIGSAAPF
QLVTELEVPLNLISTTPELFPLEYDKTLEHSGQVIRITSPTRWVVGFHAFDLARFRNVIKDPNRSSAELYRFVVHYLVLF
YCLSKSPGLGRLFEGLRYGLSFERLKGFGDLPFCVISSPVRSELPDDSVIRSSTQIAGNTSFEELVGRDNILEMNGEIRQ
RLLLTIEGL*
Nucleotide sequence: 750 bp
>ACP00009 NC_002947:c3497581-3496832 [Pseudomonas putida KT2440]
ATGACACAGACATTCGATGTCGAAGCACTGATCAAGCTGCGTAGCCAAACGCGGGCGATCTCCGATGCGCTGAAGGCGCA
GGCGGCGGATTACCTTGCCACCGTGGCACCGCTGATCCGTCCGCAAACCCTGTTTGGCGAATACCTGCAAGGCGCACAGC
GCAGCAGCGGGCGCGAAACCCAGGGCCACTTCCAGGCCCTGGTCGAACTCTACGAACGTATCGGCTCGGCCGCGCCGTTC
CAGCTGGTCACCGAGCTGGAAGTACCGCTGAACCTGATCAGCACCACCCCCGAACTGTTCCCGCTGGAATACGACAAGAC
CCTGGAACACAGCGGCCAGGTCATCCGCATCACCAGCCCGACCCGCTGGGTGGTGGGCTTCCACGCCTTCGACCTGGCGC
GGTTTCGCAATGTCATCAAAGACCCCAACCGCAGCAGCGCCGAGCTGTACCGCTTCGTGGTGCACTACCTGGTGCTGTTC
TACTGCCTGAGCAAGTCGCCTGGCCTTGGGCGGCTGTTCGAGGGCCTGCGCTACGGCCTGAGCTTCGAGCGCCTGAAGGG
CTTTGGCGACCTGCCGTTCTGCGTCATCAGCTCGCCGGTGCGCTCGGAACTTCCCGACGACTCGGTCATCCGCAGCAGCA
CGCAGATTGCCGGCAACACCAGCTTCGAAGAACTGGTAGGCCGCGACAACATCCTGGAAATGAACGGCGAAATCCGTCAG
CGCCTGCTGCTGACGATCGAAGGACTGTAA
ATGACACAGACATTCGATGTCGAAGCACTGATCAAGCTGCGTAGCCAAACGCGGGCGATCTCCGATGCGCTGAAGGCGCA
GGCGGCGGATTACCTTGCCACCGTGGCACCGCTGATCCGTCCGCAAACCCTGTTTGGCGAATACCTGCAAGGCGCACAGC
GCAGCAGCGGGCGCGAAACCCAGGGCCACTTCCAGGCCCTGGTCGAACTCTACGAACGTATCGGCTCGGCCGCGCCGTTC
CAGCTGGTCACCGAGCTGGAAGTACCGCTGAACCTGATCAGCACCACCCCCGAACTGTTCCCGCTGGAATACGACAAGAC
CCTGGAACACAGCGGCCAGGTCATCCGCATCACCAGCCCGACCCGCTGGGTGGTGGGCTTCCACGCCTTCGACCTGGCGC
GGTTTCGCAATGTCATCAAAGACCCCAACCGCAGCAGCGCCGAGCTGTACCGCTTCGTGGTGCACTACCTGGTGCTGTTC
TACTGCCTGAGCAAGTCGCCTGGCCTTGGGCGGCTGTTCGAGGGCCTGCGCTACGGCCTGAGCTTCGAGCGCCTGAAGGG
CTTTGGCGACCTGCCGTTCTGCGTCATCAGCTCGCCGGTGCGCTCGGAACTTCCCGACGACTCGGTCATCCGCAGCAGCA
CGCAGATTGCCGGCAACACCAGCTTCGAAGAACTGGTAGGCCGCGACAACATCCTGGAAATGAACGGCGAAATCCGTCAG
CGCCTGCTGCTGACGATCGAAGGACTGTAA