Detailed information of accessory protein


Summary

Accessory protein ID ACP00009 experimental
Type structure affiliated protein
Related T6SS (Type) T6SS00014 (Type i4b)
Strain Pseudomonas putida KT2440
Replicon chromosome
Related structure protein PP_RS15990 (TssA)
Related effector -
Description TagB1 plays a stabilizing role in as- sembly of the T6SS sheath
Reference [1] Bernal P, Furniss RCD, Fecht S, et al. A novel stabilization mechanism for the type VI secretion system sheath. Proc Natl Acad Sci U S A. 2021 Feb 16;118(7). pii: e2008500118. doi: 10.1073/pnas.2008500118. PMID: 33558227
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed

External database links

Locus tag (Gene) PP_RS16060 (tagB1)
Coordinates (Strand) 3496832..3497581 (-)
NCBI ID 898330878
RefSeq NC_002947
Uniprot ID A0A2V4G1L0_9PSED
KEGG ID -
PDB ID -

Pfam domain hit(s)

Domain Pfam ID E-value Aligned region

Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-249 MTQTFDVEALIKLRSQTRAISDALKAQAADYLATVAPLIRPQTLFGEYLQGAQRSSGRETQGHFQALVELYERIGSAAPFQLVTELEVPLNLISTTPELFPLEYDKTLEHSGQVIRITSPTRWVVGFHAFDLARFRNVIKDPNRSSAELYRFVVHYLVLFYCLSKSPGLGRLFEGLRYGLSFERLKGFGDLPFCVISSPVRSELPDDSVIRSSTQIAGNTSFEELVGRDNILEMNGEIRQRLLLTIEGL



Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9861 - -



  Protein sequence: 250 a.a.    

>ACP00009 NC_002947:c3497581-3496832 [Pseudomonas putida KT2440]
MTQTFDVEALIKLRSQTRAISDALKAQAADYLATVAPLIRPQTLFGEYLQGAQRSSGRETQGHFQALVELYERIGSAAPF
QLVTELEVPLNLISTTPELFPLEYDKTLEHSGQVIRITSPTRWVVGFHAFDLARFRNVIKDPNRSSAELYRFVVHYLVLF
YCLSKSPGLGRLFEGLRYGLSFERLKGFGDLPFCVISSPVRSELPDDSVIRSSTQIAGNTSFEELVGRDNILEMNGEIRQ
RLLLTIEGL*

  Nucleotide sequence: 750 bp    

>ACP00009 NC_002947:c3497581-3496832 [Pseudomonas putida KT2440]
ATGACACAGACATTCGATGTCGAAGCACTGATCAAGCTGCGTAGCCAAACGCGGGCGATCTCCGATGCGCTGAAGGCGCA
GGCGGCGGATTACCTTGCCACCGTGGCACCGCTGATCCGTCCGCAAACCCTGTTTGGCGAATACCTGCAAGGCGCACAGC
GCAGCAGCGGGCGCGAAACCCAGGGCCACTTCCAGGCCCTGGTCGAACTCTACGAACGTATCGGCTCGGCCGCGCCGTTC
CAGCTGGTCACCGAGCTGGAAGTACCGCTGAACCTGATCAGCACCACCCCCGAACTGTTCCCGCTGGAATACGACAAGAC
CCTGGAACACAGCGGCCAGGTCATCCGCATCACCAGCCCGACCCGCTGGGTGGTGGGCTTCCACGCCTTCGACCTGGCGC
GGTTTCGCAATGTCATCAAAGACCCCAACCGCAGCAGCGCCGAGCTGTACCGCTTCGTGGTGCACTACCTGGTGCTGTTC
TACTGCCTGAGCAAGTCGCCTGGCCTTGGGCGGCTGTTCGAGGGCCTGCGCTACGGCCTGAGCTTCGAGCGCCTGAAGGG
CTTTGGCGACCTGCCGTTCTGCGTCATCAGCTCGCCGGTGCGCTCGGAACTTCCCGACGACTCGGTCATCCGCAGCAGCA
CGCAGATTGCCGGCAACACCAGCTTCGAAGAACTGGTAGGCCGCGACAACATCCTGGAAATGAACGGCGAAATCCGTCAG
CGCCTGCTGCTGACGATCGAAGGACTGTAA