Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ABDZ23_RS10235 Genome accession   NZ_CP155621
Coordinates   2284521..2286221 (+) Length   566 a.a.
NCBI ID   WP_346833974.1    Uniprot ID   -
Organism   Pseudomonas koreensis strain BM06     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2279521..2291221
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABDZ23_RS10215 (ABDZ23_10215) - 2279685..2282633 (+) 2949 WP_247269214.1 DUF748 domain-containing protein -
  ABDZ23_RS10220 (ABDZ23_10220) - 2282633..2282929 (+) 297 WP_027614107.1 DUF2845 domain-containing protein -
  ABDZ23_RS10225 (ABDZ23_10225) - 2283200..2283556 (-) 357 WP_007963876.1 BON domain-containing protein -
  ABDZ23_RS10230 (ABDZ23_10230) pilA 2283852..2284292 (-) 441 WP_247269215.1 pilin Machinery gene
  ABDZ23_RS10235 (ABDZ23_10235) pilB 2284521..2286221 (+) 1701 WP_346833974.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABDZ23_RS10240 (ABDZ23_10240) pilC 2286224..2287441 (+) 1218 WP_247269217.1 type II secretion system F family protein Machinery gene
  ABDZ23_RS10245 (ABDZ23_10245) pilD 2287443..2288315 (+) 873 WP_096795219.1 A24 family peptidase Machinery gene
  ABDZ23_RS10250 (ABDZ23_10250) coaE 2288312..2288935 (+) 624 WP_346833975.1 dephospho-CoA kinase -
  ABDZ23_RS10255 (ABDZ23_10255) yacG 2288932..2289129 (+) 198 WP_003228296.1 DNA gyrase inhibitor YacG -
  ABDZ23_RS10260 (ABDZ23_10260) - 2289136..2289351 (-) 216 WP_003228298.1 hypothetical protein -
  ABDZ23_RS10265 (ABDZ23_10265) - 2289417..2290106 (-) 690 WP_135296585.1 energy-coupling factor ABC transporter permease -
  ABDZ23_RS10270 (ABDZ23_10270) - 2290224..2290850 (+) 627 WP_003228301.1 DUF1780 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62551.65 Da        Isoelectric Point: 5.8561

>NTDB_id=998084 ABDZ23_RS10235 WP_346833974.1 2284521..2286221(+) (pilB) [Pseudomonas koreensis strain BM06]
MNDIALSGLAKQLVQAELLTEKSAQQAWQQAQRNRLSLISYLVQNKLVKSSQVAEIASEHFGMAFMDLNCLDKETQPKGL
VSEKLVRQHHALPLWRRGNKLFVGISDPSNHQAINDIQFSTGLSTEAILVEDDKLTEAIEKFFDTHASGLEEMADVDLDG
VDVESVDDSRQDAIGGLDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKNFRVRVRTDGILREVAKPPIQLAGRIAARL
KVMASLDISERRKPQDGRIKMRLSKTKSIDFRVNTLPTLWGEKVVIRILDPSSAQIGIDALGYEPAQKELYMAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPKQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAEILTRLHNMGIPGFNIATSVSLIIAQRLARKLCSHCKKPIEIPRETLIKEGFPP
EQIGSFTIYEPVGCDLCNGGYKGRVGIYEVVKNTPDLQRLIMAEGNSLEIDSQMRRDGFDDLRTSGLRKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=998084 ABDZ23_RS10235 WP_346833974.1 2284521..2286221(+) (pilB) [Pseudomonas koreensis strain BM06]
ATGAATGACATCGCTCTGAGCGGTCTGGCCAAGCAATTGGTGCAGGCCGAACTGCTTACGGAAAAAAGCGCCCAGCAGGC
CTGGCAACAGGCGCAGCGCAATCGGCTGTCGCTGATCAGCTACCTGGTGCAGAACAAACTGGTGAAGAGCTCACAGGTGG
CCGAGATCGCTTCCGAGCATTTCGGCATGGCCTTCATGGACCTCAATTGCCTGGACAAGGAAACCCAGCCCAAAGGCCTG
GTCAGTGAAAAACTGGTGCGCCAGCATCACGCCCTGCCCTTGTGGCGCCGTGGCAACAAGCTGTTCGTGGGCATTTCCGA
CCCGAGCAATCATCAGGCGATCAATGACATCCAGTTCAGCACCGGGCTGAGCACCGAAGCCATTCTGGTGGAGGACGACA
AGCTCACCGAGGCCATCGAGAAATTCTTCGACACCCACGCCAGCGGCCTCGAAGAAATGGCCGACGTCGATCTCGACGGC
GTGGATGTCGAGTCCGTCGACGACAGCCGCCAGGACGCCATTGGCGGCCTCGACGCCGACGACGCGCCGGTTGTGCGCTT
CGTGCACAAGATGCTGCTCGACGCGATCAAGAGTGGCTCGTCGGACTTGCACTTCGAACCCTACGAAAAAAACTTCAGGG
TACGGGTGCGCACCGACGGCATCCTGCGTGAGGTGGCCAAACCGCCGATCCAGTTGGCCGGGCGAATCGCCGCACGCCTG
AAAGTCATGGCCAGCCTGGACATCTCGGAACGCCGCAAACCTCAGGACGGGCGGATCAAGATGCGCCTGTCGAAGACCAA
GTCGATCGACTTCCGGGTCAACACCTTGCCGACCCTGTGGGGAGAGAAAGTGGTGATCCGGATCCTCGATCCGTCCAGCG
CGCAGATCGGCATCGATGCATTGGGCTACGAACCGGCGCAGAAAGAACTGTACATGGCCGCGCTCAAGCAGCCCCAAGGG
ATGATTCTGGTTACCGGGCCTACGGGTTCGGGGAAAACCGTATCGCTGTACACCGGCCTGAATATTCTCAACACCGTCGA
CATCAACATTTCCACCGCCGAAGACCCGGTGGAAATCAACATGGAAGGCATCAACCAGGTCAACGTCAATCCGAAACAGG
GGCTGGATTTTGCTCAGGCTCTGCGTTCGTTTCTGCGTCAGGATCCGGACGTGATCATGGTCGGGGAAATCCGCGATCTG
GAAACCGCCGAAATCGCGATCAAGGCGGCGCAGACCGGCCACCTGGTGCTGTCCACGCTGCACACCAACAGTGCCGCTGA
GATTCTGACCCGCCTGCACAACATGGGCATCCCCGGGTTCAACATCGCCACCTCGGTCAGTCTGATCATTGCCCAGCGCC
TGGCGCGCAAGCTGTGCAGCCACTGCAAGAAACCGATCGAGATCCCCCGCGAAACGCTGATCAAGGAAGGTTTCCCGCCG
GAACAGATCGGCAGTTTCACGATCTATGAGCCGGTCGGTTGCGACCTGTGCAACGGCGGCTACAAGGGACGCGTGGGGAT
TTACGAAGTGGTGAAAAACACCCCTGATCTGCAACGGCTGATCATGGCCGAAGGCAACTCGCTGGAGATCGACAGCCAGA
TGCGTCGCGACGGCTTCGACGACCTGCGCACCTCCGGCCTGCGCAAGGCCATGCAAGGCATCACCAGCCTGGAAGAAATC
AACCGGGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

57.522

99.823

0.574
  pilB Acinetobacter baumannii D1279779

57.092

99.647

0.569
  pilB Legionella pneumophila strain ERS1305867

55.3

100

0.553
  pilB Vibrio cholerae strain A1552

50.796

99.823

0.507
  pilB Vibrio campbellii strain DS40M4

49.209

100

0.495
  pilB Vibrio parahaemolyticus RIMD 2210633

49.733

99.117

0.493
  pilF Neisseria gonorrhoeae MS11

49.201

99.47

0.489
  pilF Thermus thermophilus HB27

36.918

98.587

0.364

Multiple sequence alignment