Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ABFB52_RS03115 Genome accession   NZ_CP155585
Coordinates   693694..695772 (+) Length   692 a.a.
NCBI ID   WP_129972287.1    Uniprot ID   -
Organism   Pseudomonas sp. 9.2(2024)     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 688694..700772
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABFB52_RS03095 (ABFB52_03095) comM 690908..691972 (+) 1065 WP_085746416.1 pilus assembly protein PilM Machinery gene
  ABFB52_RS03100 (ABFB52_03100) - 691972..692538 (+) 567 WP_085746417.1 PilN domain-containing protein -
  ABFB52_RS03105 (ABFB52_03105) pilO 692535..693158 (+) 624 WP_085746418.1 type 4a pilus biogenesis protein PilO -
  ABFB52_RS03110 (ABFB52_03110) - 693155..693679 (+) 525 WP_074689563.1 pilus assembly protein PilP -
  ABFB52_RS03115 (ABFB52_03115) pilQ 693694..695772 (+) 2079 WP_129972287.1 type IV pilus secretin PilQ Machinery gene
  ABFB52_RS03120 (ABFB52_03120) aroK 695777..696295 (+) 519 WP_045121685.1 shikimate kinase AroK -
  ABFB52_RS03125 (ABFB52_03125) aroB 696446..697546 (+) 1101 WP_129972288.1 3-dehydroquinate synthase -
  ABFB52_RS03130 (ABFB52_03130) - 697557..699164 (+) 1608 WP_085746420.1 AAA family ATPase -

Sequence

Protein


Download         Length: 692 a.a.        Molecular weight: 75039.23 Da        Isoelectric Point: 5.5724

>NTDB_id=997950 ABFB52_RS03115 WP_129972287.1 693694..695772(+) (pilQ) [Pseudomonas sp. 9.2(2024)]
MNRIFSTLGFSLWMALLSPMVQAANLKALDVAALPGDRVELKLSFDGPPPQPKGYTTDSPARIALDLPGVASQLASKTRD
LGSGNARTATVVEANNRTRLIISLTQLAPYDTRVEGNTVFVVVGQGSKAVALRTAARAPRAAPAAAPVRTFAPTARAIRG
VDFQRGTAGEGNVVIDLSDPAIAPDIQEHDGKIILNFARTQLPEPLRVRLDVKDFATPVQFVNAGVSGDRATITVEPSGT
FDYSTYQTDNKLTVSIRPLTVDDLQKRNADRNSYNGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQNV
PWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTSAEAK
VDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNKGNWNTSGVSN
GSSTTIGTPGSTSTNSPFVDMGTVNNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPY
QEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFSN
TQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2079 bp        

>NTDB_id=997950 ABFB52_RS03115 WP_129972287.1 693694..695772(+) (pilQ) [Pseudomonas sp. 9.2(2024)]
ATGAACAGGATTTTTTCCACCCTCGGTTTTTCGCTATGGATGGCGCTGCTTTCACCGATGGTACAGGCAGCCAATCTGAA
GGCGCTGGACGTCGCGGCACTGCCGGGCGACCGGGTCGAGCTGAAGCTGTCGTTCGACGGTCCGCCCCCGCAGCCCAAGG
GCTACACCACCGATTCGCCGGCGCGGATCGCCCTCGATCTGCCGGGTGTCGCCAGTCAGCTGGCGAGCAAGACTCGCGAT
CTGGGCAGTGGCAATGCCCGCACGGCCACGGTGGTCGAGGCCAATAACCGGACGCGGCTGATCATCAGCCTGACGCAACT
GGCGCCGTACGACACCCGGGTCGAAGGCAATACGGTGTTCGTGGTGGTCGGGCAAGGCAGCAAAGCCGTTGCCCTGCGTA
CGGCGGCTCGTGCGCCGCGCGCAGCACCTGCGGCAGCGCCGGTCCGCACGTTTGCGCCGACCGCCAGGGCCATTCGCGGC
GTCGATTTCCAGCGTGGTACGGCAGGTGAGGGCAATGTGGTCATTGATCTGTCCGACCCGGCCATTGCCCCGGACATTCA
GGAACACGACGGCAAGATCATCCTCAATTTCGCGCGTACCCAATTGCCCGAGCCGCTGCGGGTGCGCCTCGACGTCAAGG
ATTTCGCCACCCCGGTGCAGTTCGTCAATGCCGGTGTCAGCGGTGATCGGGCGACGATCACCGTCGAACCCAGCGGTACT
TTCGACTATTCCACTTACCAGACCGACAACAAGCTGACTGTCAGCATCCGGCCGTTGACCGTCGATGACCTGCAAAAGCG
CAACGCCGACCGCAACAGCTATAACGGCGAAAAGCTCTCTCTCAACTTTCAGGACATCGATGTGCGTTCGGTGCTGCAAC
TGATCGCCGACTTCACCAACCTCAATCTGGTGGCCAGCGATACGGTGCAGGGCGGCATTACCTTGCGCCTGCAGAACGTG
CCGTGGGATCAGGCGCTGGATCTGGTGCTCAAGACCAAAGGCCTGGACAAGCGCAAGGTCGGCAATGTGCTGCTGGTGGC
ACCGGCAGATGAAATCGCCGCCCGCGAACGGCAGGAACTGGAATCGCAGAAACAGATCGCCGAACTGGCGCCGTTGCGCC
GCGAGCTGCTGCAAGTGAACTACGCCAAGGCTGCGGACATCGCCAAGCTGTTCCAGTCGGTCACCAGTGCCGAGGCCAAG
GTCGACGAACGGGGTTCGATCACCGTCGATGAGCGCACCAACAACATCATTGCCTACCAGACCCAGGATCGCCTCGACGA
GCTGCGGCGGATTGTGGCGCAGCTGGATATTCCGGTGCGTCAAGTGATGATCGAGGCGCGGATCGTCGAGGCCAACGTCG
ATTACGACAAGAGCCTGGGTGTGCGTTGGGGCGGCTCGATCCAGAACAAGGGCAACTGGAACACTTCCGGGGTCAGCAAT
GGCTCGTCGACCACCATCGGCACGCCGGGCAGCACCAGCACCAATTCGCCGTTCGTCGACATGGGCACGGTCAATAACAC
GTCCGGGATCGGCATCGCGTTCATCACCGACAACGTCTTGCTCGACCTTGAGCTGACCGCGATGGAGAAGACCGGCAACG
GCGAAATCGTCTCGCAGCCCAAAGTGGTCACCTCCGACAAGGAAACCGCAAAGATCCTCAAGGGCACCGAGATTCCGTAT
CAGGAAGCCAGCTCCAGCGGCGCTACGTCGGTGTCCTTCAAGGAGGCGTCGCTGTCGCTGGAAGTGACGCCGCAGATCAC
CCCGGACAACCGCATCATCATGGAGGTCAAGGTCACCAAGGACGAACCGGACTACCTGAACAAAGTGCAGGATGTACCGC
CGATCAAGAAAAACGAGGTCAATGCCAAGGTGCTGGTGAACGACGGCGAGACCATCGTGATCGGGGGCGTTTTCTCAAAT
ACTCAGAGCAAGGTTGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGTGGT
TTCGGAGAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(376-441)

Bacterial type II and III secretion system protein (Secretin)

(527-683)

AMIN domain (AMIN)

(37-121)

Secretin and TonB N terminus short domain (STN)

(302-348)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

71.329

100

0.737
  comQ Acinetobacter baylyi ADP1

40.303

100

0.423
  pilQ Acinetobacter baumannii strain A118

37.383

100

0.405
  pilQ Acinetobacter baumannii D1279779

37.383

100

0.405
  pilQ Legionella pneumophila strain Lp02

37.63

97.543

0.367
  pilQ Legionella pneumophila strain ERS1305867

37.593

97.254

0.366

Multiple sequence alignment