Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   ABFB52_RS03095 Genome accession   NZ_CP155585
Coordinates   690908..691972 (+) Length   354 a.a.
NCBI ID   WP_085746416.1    Uniprot ID   -
Organism   Pseudomonas sp. 9.2(2024)     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 685908..696972
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABFB52_RS03085 (ABFB52_03085) - 686623..687891 (+) 1269 WP_007952395.1 malic enzyme-like NAD(P)-binding protein -
  ABFB52_RS03090 (ABFB52_03090) - 688243..690690 (-) 2448 WP_085746415.1 penicillin-binding protein 1A -
  ABFB52_RS03095 (ABFB52_03095) comM 690908..691972 (+) 1065 WP_085746416.1 pilus assembly protein PilM Machinery gene
  ABFB52_RS03100 (ABFB52_03100) - 691972..692538 (+) 567 WP_085746417.1 PilN domain-containing protein -
  ABFB52_RS03105 (ABFB52_03105) pilO 692535..693158 (+) 624 WP_085746418.1 type 4a pilus biogenesis protein PilO -
  ABFB52_RS03110 (ABFB52_03110) - 693155..693679 (+) 525 WP_074689563.1 pilus assembly protein PilP -
  ABFB52_RS03115 (ABFB52_03115) pilQ 693694..695772 (+) 2079 WP_129972287.1 type IV pilus secretin PilQ Machinery gene
  ABFB52_RS03120 (ABFB52_03120) aroK 695777..696295 (+) 519 WP_045121685.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38124.63 Da        Isoelectric Point: 4.6857

>NTDB_id=997949 ABFB52_RS03095 WP_085746416.1 690908..691972(+) (comM) [Pseudomonas sp. 9.2(2024)]
MLGLFNKKANTLLGIDISSTSVKLLELSRQGERYRVEAYAVEPLPANAVVEKNIAELEGVGQALSRVLVKARTGLKTVAV
AVAGSAVITKVIEMDAGLSDDELENQLKIEADQYIPYPLDEVAIDFEVQGVSPRNPERVNVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLATQLAASQDRLTVAVVDIGATMTTLSVLHNGRIIYTREQLFGGRQLTEEIQRRYGLTL
EQAGLAKKQGGLPDDYVSEVLQPFREALVQQVSRSLQFFFASGQYNAVDHILLAGGTASVPGLDRLIEERLNTPTQVANP
FSDMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=997949 ABFB52_RS03095 WP_085746416.1 690908..691972(+) (comM) [Pseudomonas sp. 9.2(2024)]
GTGCTAGGACTCTTCAATAAAAAGGCCAATACGTTACTGGGGATCGATATCAGCTCCACGTCGGTGAAGCTACTGGAGCT
GAGCCGTCAGGGGGAGCGCTACCGGGTCGAGGCGTACGCGGTGGAGCCGTTGCCGGCCAACGCCGTGGTCGAAAAGAACA
TCGCCGAGCTCGAGGGCGTTGGCCAGGCTCTGTCCCGGGTGCTGGTCAAGGCGCGCACCGGGCTGAAAACCGTGGCGGTG
GCGGTGGCTGGATCTGCGGTGATCACCAAGGTCATCGAGATGGACGCCGGGCTGTCCGACGACGAGCTGGAAAACCAGCT
CAAGATCGAAGCCGATCAATACATTCCCTATCCCCTGGATGAGGTCGCCATCGACTTCGAAGTCCAGGGCGTTTCGCCGC
GCAACCCCGAACGGGTCAACGTGCTGCTGGCCGCCTGTCGCAAGGAAAACGTCGAAGTCCGCGAAGCGGCGCTTGCACTG
GCCGGGCTGACGGCGCGGGTGGTGGATGTCGAGGCTTATGCGCTGGAGCGCTCGTTCGGCCTGCTGGCCACGCAACTGGC
CGCGTCACAGGATCGGCTGACGGTGGCCGTGGTCGACATCGGCGCCACCATGACCACCCTCAGCGTCCTGCACAACGGCC
GGATCATCTATACCCGCGAACAGCTGTTCGGCGGTCGACAGCTGACCGAGGAAATTCAGCGCCGTTATGGCCTGACCCTT
GAGCAGGCGGGGCTCGCCAAGAAACAGGGCGGTCTGCCGGACGACTACGTCAGCGAGGTGCTGCAACCGTTTCGCGAGGC
GCTGGTGCAGCAGGTGTCGCGCTCCCTGCAGTTCTTTTTCGCCTCCGGCCAGTACAACGCCGTCGACCATATTCTGCTGG
CCGGCGGCACGGCGTCGGTGCCCGGGCTCGATCGGCTGATCGAAGAGCGTCTGAACACCCCGACCCAGGTCGCCAACCCG
TTTTCCGACATGGCCTTGAGCAGCAAGGTCAACGCCGGGGCCCTGGCGAGCGATGCGCCGGCCCTGATGATTGCCTGCGG
GCTCGCGCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Acinetobacter nosocomialis M2

56.215

100

0.562
  pilM Acinetobacter baumannii D1279779

56.215

100

0.562
  comM Acinetobacter baylyi ADP1

54.237

100

0.542
  pilM Legionella pneumophila strain ERS1305867

49.296

100

0.494

Multiple sequence alignment