Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ABDK11_RS14005 Genome accession   NZ_CP155567
Coordinates   3224976..3226688 (+) Length   570 a.a.
NCBI ID   WP_346837134.1    Uniprot ID   -
Organism   Microbulbifer sp. SAOS-129_SWC     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3219976..3231688
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABDK11_RS13990 (ABDK11_13990) - 3220992..3221960 (-) 969 WP_346836609.1 IS30 family transposase -
  ABDK11_RS13995 (ABDK11_13995) - 3222046..3223845 (-) 1800 WP_346837132.1 phosphoethanolamine transferase -
  ABDK11_RS14000 (ABDK11_14000) pilE 3224094..3224558 (-) 465 WP_346837133.1 pilin Machinery gene
  ABDK11_RS14005 (ABDK11_14005) pilB 3224976..3226688 (+) 1713 WP_346837134.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABDK11_RS14010 (ABDK11_14010) pilC 3226814..3228025 (+) 1212 WP_346837135.1 type II secretion system F family protein Machinery gene
  ABDK11_RS14015 (ABDK11_14015) - 3228045..3228929 (+) 885 WP_346837136.1 A24 family peptidase -
  ABDK11_RS14020 (ABDK11_14020) coaE 3229172..3229771 (+) 600 WP_346837137.1 dephospho-CoA kinase -
  ABDK11_RS14025 (ABDK11_14025) yacG 3229781..3229996 (+) 216 WP_346837138.1 DNA gyrase inhibitor YacG -
  ABDK11_RS14030 (ABDK11_14030) mutT 3230059..3230460 (-) 402 WP_346837139.1 8-oxo-dGTP diphosphatase MutT -
  ABDK11_RS14035 (ABDK11_14035) argJ 3230471..3231688 (-) 1218 WP_346837140.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 62398.08 Da        Isoelectric Point: 5.0283

>NTDB_id=997800 ABDK11_RS14005 WP_346837134.1 3224976..3226688(+) (pilB) [Microbulbifer sp. SAOS-129_SWC]
MSSTPLSGLAKRLVADQILDEATAVSASKAARRENQTFAQHAVEAKLIKSRELASIASLAFGSPLFDLGSYNFELIPRDI
LDEKLISKHFALPLYKRGNRLFVAVADPTNLAGLDEINFNTGLNTDAVLVEADKLAKAIDSYLSDNGDMGSGLEGMDDED
LDGLEVGADDSDRQQDDDPGGDEAPVVRFVNKVLLDAIRTGASDIHFEPYEKSYRVRLRTDGVLHEVAKPPIQLAARISA
RLKVMSKMDISERRVPQDGRIKMKLSKTKAIDFRVNSLPTLWGEKIVLRILDPSSAKLGIDALGYEDEQKKIYMDALAQP
QGMILVTGPTGSGKTVSLYTGLNILNTPERNISTAEDPVEINLEGINQVNVSNKVGLNFAEALRSFLRQDPDIVMVGEIR
DLETAEIAIKAAQTGHLVLSTLHTNSAPETLTRLMNMGVPTFNIATSVSVIIAQRLARRLCNECKKPVNLPDEVLEEEGF
DAVTIDRKEWKIYQPVGCEHCSKGYKGRVGVYEVVRITDGISRIIMEGGNSIQIADQARVEGFNNLRTSALRKVVGGITS
LEEANRVTKD

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=997800 ABDK11_RS14005 WP_346837134.1 3224976..3226688(+) (pilB) [Microbulbifer sp. SAOS-129_SWC]
ATGAGCAGCACTCCCCTGAGCGGGCTGGCCAAACGACTGGTCGCCGATCAAATTCTGGACGAGGCCACGGCCGTTTCGGC
CTCCAAGGCCGCCCGCCGTGAAAACCAGACCTTCGCCCAGCATGCCGTCGAGGCCAAGCTGATCAAGAGCCGCGAGTTGG
CTAGTATCGCCTCCCTGGCATTCGGCTCACCGCTGTTCGATCTGGGCAGCTACAACTTTGAACTGATCCCGCGCGACATT
CTTGATGAAAAATTAATCAGCAAGCATTTTGCGCTGCCTCTCTATAAGCGGGGCAACCGGCTATTTGTCGCGGTGGCCGA
TCCGACCAACCTCGCGGGTCTGGACGAGATCAATTTCAATACCGGCCTCAATACCGATGCGGTGCTGGTGGAGGCCGACA
AGCTGGCCAAGGCCATCGACAGCTATCTATCCGATAACGGCGATATGGGCAGCGGCCTCGAGGGCATGGACGACGAGGAT
CTCGATGGCCTTGAAGTGGGTGCAGATGATTCCGACCGGCAGCAGGATGATGATCCCGGCGGCGATGAGGCCCCGGTTGT
CCGTTTCGTGAACAAGGTGCTGCTGGATGCGATCCGTACCGGAGCATCGGATATCCACTTCGAACCCTATGAGAAGAGCT
ACCGGGTGCGCCTGCGCACTGATGGCGTACTGCACGAAGTGGCCAAACCACCGATTCAGTTGGCAGCACGTATCTCCGCG
CGCTTGAAGGTCATGTCCAAGATGGATATTTCCGAGCGCCGGGTCCCCCAGGATGGCCGTATCAAGATGAAGTTGTCCAA
GACCAAGGCCATCGACTTCCGGGTCAACAGCCTGCCGACGCTGTGGGGAGAGAAAATCGTACTGCGGATTCTCGATCCCT
CCTCGGCCAAACTCGGGATCGATGCGCTTGGCTACGAGGATGAGCAGAAAAAGATCTATATGGATGCCCTGGCCCAACCA
CAGGGGATGATCCTCGTCACCGGGCCCACCGGTTCCGGCAAAACGGTTTCGCTCTATACCGGCCTGAATATCCTCAACAC
CCCCGAGCGCAATATCTCCACCGCGGAGGATCCGGTGGAAATCAACCTGGAGGGTATCAACCAGGTCAACGTCTCCAACA
AGGTGGGGCTCAACTTCGCCGAGGCGTTACGCTCTTTCCTGCGTCAGGACCCGGATATCGTCATGGTTGGTGAGATTCGT
GACCTGGAGACGGCGGAAATCGCCATCAAGGCGGCGCAGACCGGCCACCTGGTTTTATCCACACTGCACACCAACTCTGC
ACCGGAGACATTGACCCGTCTGATGAATATGGGCGTGCCCACGTTCAACATCGCCACCTCCGTCAGCGTGATCATCGCCC
AGCGACTGGCACGACGCCTGTGTAACGAGTGCAAAAAACCGGTCAACCTGCCGGATGAAGTGCTGGAGGAAGAGGGATTT
GATGCGGTCACCATTGACCGCAAGGAGTGGAAAATATACCAGCCCGTGGGCTGTGAGCACTGCTCCAAGGGTTACAAGGG
GCGAGTGGGTGTGTATGAAGTGGTTCGCATCACCGATGGCATCTCAAGAATTATAATGGAAGGGGGCAATTCTATTCAGA
TTGCCGACCAGGCTCGCGTCGAAGGGTTTAACAACCTGCGCACCTCGGCATTACGCAAGGTCGTAGGCGGTATTACCAGC
CTGGAAGAAGCGAACCGGGTAACCAAAGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

60.245

100

0.604
  pilB Acinetobacter baylyi ADP1

59.545

100

0.596
  pilB Legionella pneumophila strain ERS1305867

56.085

99.474

0.558
  pilB Vibrio cholerae strain A1552

52.05

98.421

0.512
  pilB Vibrio parahaemolyticus RIMD 2210633

54.667

92.105

0.504
  pilB Vibrio campbellii strain DS40M4

53.497

92.807

0.496
  pilF Neisseria gonorrhoeae MS11

47.986

100

0.481
  pilB/pilB1 Synechocystis sp. PCC 6803

41.6

87.719

0.365

Multiple sequence alignment