Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   AAHN93_RS11355 Genome accession   NZ_CP154474
Coordinates   2547571..2548110 (-) Length   179 a.a.
NCBI ID   WP_343292555.1    Uniprot ID   -
Organism   Vandammella animalimorsus strain W29-2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2542571..2553110
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAHN93_RS11335 (AAHN93_11335) - 2542763..2544046 (+) 1284 WP_343292551.1 glutamate-5-semialdehyde dehydrogenase -
  AAHN93_RS11340 (AAHN93_11340) argS 2544131..2545813 (+) 1683 WP_343292552.1 arginine--tRNA ligase -
  AAHN93_RS11345 (AAHN93_11345) - 2545933..2546649 (+) 717 WP_343292553.1 SPOR domain-containing protein -
  AAHN93_RS11350 (AAHN93_11350) - 2546712..2547482 (-) 771 WP_343292554.1 hypothetical protein -
  AAHN93_RS11355 (AAHN93_11355) pilA 2547571..2548110 (-) 540 WP_343292555.1 pilin Machinery gene
  AAHN93_RS11360 (AAHN93_11360) - 2548445..2549146 (-) 702 WP_124857713.1 FHA domain-containing protein -
  AAHN93_RS11365 (AAHN93_11365) recA 2549571..2550638 (+) 1068 WP_095552384.1 recombinase RecA Machinery gene
  AAHN93_RS11370 (AAHN93_11370) recX 2550638..2551330 (+) 693 WP_343292556.1 recombination regulator RecX -
  AAHN93_RS11375 (AAHN93_11375) - 2551423..2551737 (+) 315 WP_343292557.1 hypothetical protein -
  AAHN93_RS11380 (AAHN93_11380) - 2551861..2552676 (-) 816 WP_343292558.1 exosortase H-associated membrane protein -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 17749.23 Da        Isoelectric Point: 7.7744

>NTDB_id=993558 AAHN93_RS11355 WP_343292555.1 2547571..2548110(-) (pilA) [Vandammella animalimorsus strain W29-2]
MKASLQKGFTLIELMIVVAIIGILAAVALPAYQDYTVRARVTEGLSLAGAAKTTIAENASNGTPFAQGYAGTAPTRSVTG
NTGAGTATAAVTTPGASGVNIDPTTGEITIEYQPNVAAAGANVLTIAPSSNGAALVAGTPPLTSIRWDCYAAGVPARAGG
VGPTFAPTLLAKYAPAECR

Nucleotide


Download         Length: 540 bp        

>NTDB_id=993558 AAHN93_RS11355 WP_343292555.1 2547571..2548110(-) (pilA) [Vandammella animalimorsus strain W29-2]
ATGAAAGCATCTCTGCAAAAAGGTTTCACCCTGATCGAACTGATGATCGTTGTGGCCATCATCGGTATCCTGGCTGCTGT
GGCACTGCCTGCCTATCAGGACTATACGGTGCGTGCCCGCGTTACTGAAGGTCTGTCGCTGGCTGGCGCGGCTAAAACAA
CGATTGCCGAAAATGCGAGCAACGGCACTCCGTTCGCTCAAGGCTACGCTGGTACAGCCCCCACCCGCTCGGTCACTGGT
AATACCGGTGCTGGTACCGCCACCGCCGCTGTGACCACGCCTGGTGCCTCTGGTGTGAACATTGATCCGACTACTGGTGA
AATCACGATTGAATACCAGCCTAACGTTGCTGCTGCCGGTGCCAACGTGTTGACCATCGCTCCCAGCTCCAACGGTGCCG
CTCTGGTTGCTGGTACTCCTCCCTTGACGAGCATCCGTTGGGATTGCTATGCAGCCGGTGTTCCGGCGCGTGCTGGTGGC
GTGGGCCCCACTTTCGCCCCGACCCTGCTGGCCAAATACGCACCTGCTGAGTGCCGTTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(37-179)

Prokaryotic N-terminal methylation motif (N_methyl)

(6-29)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

47.778

100

0.48
  pilA2 Legionella pneumophila str. Paris

45.304

100

0.458
  pilA2 Legionella pneumophila strain ERS1305867

44.751

100

0.453
  comP Acinetobacter baylyi ADP1

39.011

100

0.397
  pilE Neisseria gonorrhoeae strain FA1090

37.222

100

0.374

Multiple sequence alignment