Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QMG46_RS09560 Genome accession   NZ_AP027042
Coordinates   2168246..2169967 (-) Length   573 a.a.
NCBI ID   WP_281852281.1    Uniprot ID   -
Organism   Dyella sp. GSA-30     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2163246..2174967
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QMG46_RS09535 (DYGSA30_18930) - 2163650..2164138 (+) 489 WP_281852276.1 dihydrofolate reductase -
  QMG46_RS09540 (DYGSA30_18940) - 2164156..2165091 (+) 936 WP_281852277.1 Nudix family hydrolase -
  QMG46_RS09545 (DYGSA30_18950) coaE 2165286..2165906 (-) 621 WP_281852278.1 dephospho-CoA kinase -
  QMG46_RS09550 (DYGSA30_18960) pilD 2165903..2166760 (-) 858 WP_281852279.1 A24 family peptidase Machinery gene
  QMG46_RS09555 (DYGSA30_18970) pilC 2166831..2168099 (-) 1269 WP_281852280.1 type II secretion system F family protein Machinery gene
  QMG46_RS09560 (DYGSA30_18980) pilB 2168246..2169967 (-) 1722 WP_281852281.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QMG46_RS09565 (DYGSA30_18990) - 2170171..2171343 (+) 1173 WP_281852282.1 DegT/DnrJ/EryC1/StrS family aminotransferase -
  QMG46_RS09570 (DYGSA30_19000) - 2171416..2172270 (+) 855 WP_281852283.1 glycosyltransferase family 2 protein -
  QMG46_RS09575 (DYGSA30_19010) - 2172263..2173477 (+) 1215 WP_281852284.1 glycosyltransferase family 4 protein -
  QMG46_RS09580 (DYGSA30_19020) - 2173474..2174511 (+) 1038 WP_281852285.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 573 a.a.        Molecular weight: 62385.96 Da        Isoelectric Point: 6.5162

>NTDB_id=99251 QMG46_RS09560 WP_281852281.1 2168246..2169967(-) (pilB) [Dyella sp. GSA-30]
MSSQLQPSLAGLSGMARRLVSEGVLPEADVRKAVADSTQQKASLAAWLLDKGLVDSPRLTQVASAEFGMPMMDVSALNPA
TMPHNMISEALINKHQALPLFKRGKRLFVGIADPMQSHALDEIKFNSNCMVEPILVERGQLIRVIENLLNTMSNAVPDMG
GGDLDELALEGGDEDAEQTGIDANANDDAPVVKFVNKILVDAIRRGASDIHFEPFETQYRVRLRMDGMLRAVASPPMKLA
SRISSRLKVMSGLDIAERRVPQDGRIKLNLSKSRAVDFRVSTLPTLFGEKIVLRILDGSSARLGIDKLGYDEPQKNLYVD
AIHKPYGMVLVTGPTGSGKTVSLYTALNILNTAERNISTVEDPVEIRVEGINQVQQNTKRGMTFAAALRSFLRQDPDVIM
VGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLHDCKKEMDISPQLLL
AAGFSQADIDAGLKVYEAVGCDACNEGYKGRVGIYQVMPMLEDIQKIILQGGNALQIAEVSRKAGVNDLRASALEKVKQG
VTSLAEIDRVTKD

Nucleotide


Download         Length: 1722 bp        

>NTDB_id=99251 QMG46_RS09560 WP_281852281.1 2168246..2169967(-) (pilB) [Dyella sp. GSA-30]
ATGTCATCGCAATTGCAACCATCGTTAGCTGGCCTCTCGGGCATGGCTCGGCGGCTGGTCTCGGAAGGCGTCCTGCCTGA
GGCCGACGTGCGCAAGGCAGTGGCGGACTCCACCCAGCAAAAGGCCAGCCTGGCCGCATGGCTGTTGGATAAGGGCCTGG
TCGACAGCCCCAGGCTGACCCAGGTCGCGTCTGCCGAATTCGGCATGCCGATGATGGACGTCTCGGCGTTGAATCCCGCC
ACCATGCCGCACAACATGATCAGCGAGGCGCTGATCAACAAGCATCAGGCGCTGCCACTGTTCAAGCGCGGCAAGCGCTT
GTTCGTCGGTATCGCCGACCCGATGCAGTCGCATGCATTGGACGAGATCAAGTTCAACTCCAATTGCATGGTCGAGCCGA
TCCTGGTCGAACGTGGCCAGTTGATCCGGGTTATCGAGAACCTGCTCAATACGATGAGCAATGCCGTGCCCGATATGGGC
GGCGGCGATCTGGACGAGCTGGCGCTGGAAGGCGGCGACGAAGATGCCGAGCAGACCGGCATCGACGCCAACGCCAACGA
CGATGCGCCGGTGGTGAAGTTCGTCAACAAGATCCTGGTGGACGCGATCCGCCGGGGTGCCTCGGATATCCACTTCGAAC
CGTTCGAGACACAGTACCGGGTGCGTCTGCGCATGGACGGCATGCTGCGTGCCGTAGCCAGCCCGCCGATGAAGCTGGCG
TCGCGTATCTCCTCGCGCTTGAAGGTGATGTCCGGCCTGGACATTGCCGAGCGTCGCGTACCGCAGGACGGTCGCATCAA
GCTGAATCTGTCCAAGAGCCGCGCGGTCGACTTCCGTGTCAGCACGTTGCCGACTTTGTTCGGCGAAAAGATCGTGCTGC
GTATCCTGGACGGCTCCTCGGCGAGGCTGGGTATCGACAAGCTCGGTTATGACGAACCCCAGAAAAATCTGTATGTCGAC
GCCATCCACAAGCCTTACGGCATGGTGTTGGTGACCGGCCCGACCGGCTCGGGTAAAACCGTGTCGTTGTATACGGCGCT
CAATATCCTGAACACGGCCGAGCGCAATATCTCGACTGTCGAAGACCCGGTCGAAATCCGCGTCGAAGGCATCAACCAGG
TCCAGCAGAACACCAAGCGCGGCATGACCTTCGCTGCCGCATTGCGCTCGTTCCTGCGCCAGGATCCAGACGTGATCATG
GTCGGCGAAATTCGCGACCTGGAAACCGCCGAGATTGCCATCAAGGCAGCGCAGACCGGCCATATGGTCCTGTCCACCCT
GCATACCAACGATGCCCCGCAGACCATCGCGCGCCTGATGAACATGGGCATCGCGCCCTATAACATCACCTCGTCGGTCA
CCCTGGTCATTGCCCAGCGTCTGGCTCGCCGCCTGCATGACTGCAAGAAAGAGATGGATATCTCGCCACAGCTGCTGCTC
GCCGCGGGCTTTAGCCAGGCCGATATCGACGCTGGACTGAAGGTGTACGAGGCGGTCGGCTGCGATGCCTGCAATGAGGG
CTATAAAGGCCGCGTAGGTATCTATCAAGTCATGCCGATGCTCGAAGATATCCAGAAAATCATCCTGCAAGGCGGTAACG
CCCTGCAGATTGCGGAGGTCTCGCGCAAGGCCGGGGTGAACGACCTGCGCGCCTCGGCGCTGGAGAAGGTAAAACAAGGA
GTTACCAGTCTTGCCGAGATCGACCGCGTTACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(48-151)

Type II/IV secretion system protein (T2SSE)

(197-464)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.007

98.778

0.553
  pilB Acinetobacter baylyi ADP1

54.255

98.429

0.534
  pilB Legionella pneumophila strain ERS1305867

52.014

99.651

0.518
  pilB Vibrio cholerae strain A1552

50.376

92.845

0.468
  pilF Neisseria gonorrhoeae MS11

47.34

98.429

0.466
  pilB Vibrio parahaemolyticus RIMD 2210633

49.159

93.368

0.459
  pilB Vibrio campbellii strain DS40M4

48.502

93.194

0.452
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.11

95.288

0.382
  pilF Thermus thermophilus HB27

37.478

99.651

0.373

Multiple sequence alignment