Detailed information NTG0989064

Bioinformatically predicted

Overview

• Name: comEA/celA/cilE
• Type: Machinery gene
• Locus tag: AAHH81_RS04395
• Genome accession: NZ_CP152418
• Coordinates: 856682..857332 (+)
• Length: 216 a.a.
• NCBI ID: WP_057546897.1
• Uniprot ID: A0A4J1WRN7
• Organism: Streptococcus pneumoniae strain 20234287
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 851682..862332

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AAHH81_RS04365 (AAHH81_04350)	-	852165..852380 (+)	216	WP_001232081.1	YozE family protein	-
AAHH81_RS04370 (AAHH81_04355)	-	852466..853434 (+)	969	WP_000658191.1	PhoH family protein	-
AAHH81_RS04375 (AAHH81_04360)	-	853627..854127 (+)	501	WP_001866231.1	GNAT family N-acetyltransferase	-
AAHH81_RS04380 (AAHH81_04365)	-	854130..854456 (+)	327	Protein_862	TfoX/Sxy family protein	-
AAHH81_RS04385 (AAHH81_04370)	ald	854757..855868 (-)	1112	Protein_863	alanine dehydrogenase	-
AAHH81_RS04390 (AAHH81_04375)	-	856045..856614 (+)	570	WP_061813581.1	GNAT family N-acetyltransferase	-
AAHH81_RS04395 (AAHH81_04380)	comEA/celA/cilE	856682..857332 (+)	651	WP_057546897.1	ComEA family DNA-binding protein	Machinery gene
AAHH81_RS04400 (AAHH81_04385)	comEC/celB	857316..859556 (+)	2241	WP_061813580.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
AAHH81_RS04405 (AAHH81_04390)	-	859735..859923 (+)	189	WP_061813579.1	hypothetical protein	-
AAHH81_RS04410 (AAHH81_04395)	-	859956..860543 (+)	588	WP_000939895.1	ATP-binding cassette domain-containing protein	-
AAHH81_RS04415 (AAHH81_04400)	-	860547..861731 (+)	1185	WP_061813578.1	hypothetical protein	-

Sequence

Protein

• Length: 216 a.a.
• Molecular weight: 23238.66 Da
• Isoelectric Point: 5.9204

>NTDB_id=989064 AAHH81_RS04395 WP_057546897.1 856682..857332(+) (comEA/celA/cilE) [Streptococcus pneumoniae strain 20234287]
MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTPVKETNLQAEVAAVSKDLVSEKEVNKEEKEEPLEQDLITVDVKG
AVKSPGIYDLPVGSRVNDAVHKAGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGLGTASSISKEKKVNLNKA
SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGGKTIEKLKDYVTVD

Nucleotide

• Length: 651 bp

>NTDB_id=989064 AAHH81_RS04395 WP_057546897.1 856682..857332(+) (comEA/celA/cilE) [Streptococcus pneumoniae strain 20234287]
ATGGAAGCAATTATCGAGAAAATCAAAGAGTATAAAATCATCGTCATCTGTACTGGTCTGGGCTTGCTTGTAGGAGGATT
TTTCCTGCTAAAACCAGCTCCACAAACACCTGTCAAAGAGACGAATTTGCAGGCTGAAGTCGCAGCTGTTTCCAAGGATT
TGGTATCCGAAAAGGAAGTGAACAAGGAAGAAAAGGAAGAACCCCTTGAACAAGATCTAATCACAGTAGATGTTAAAGGT
GCTGTCAAATCGCCAGGGATTTATGACTTGCCTGTAGGTAGTCGAGTCAATGATGCTGTTCATAAGGCTGGTGGCTTGAC
AGAGCAAGCAGACAGCAAGTCGCTCAATCTAGCTCAGAAAGTTAGTGATGAGGCTCTGGTTTACGTTCCTACTAAGGGAG
AAGAAGCAGTTAGCCAACAGACTGGTTTGGGGACAGCTTCTTCAATAAGCAAGGAAAAGAAGGTCAATCTCAACAAGGCC
AGTCTGGAAGAACTCAAGCAGGTCAAGGGACTGGGAGGAAAACGAGCTCAGGACATTATCGACCATCGTGAGGCAAATGG
CAAGTTCAAGTCAGTAGACGAGCTCAAGAAGGTCTCTGGCATTGGTGGCAAAACAATAGAAAAGCTTAAAGACTATGTTA
CAGTGGATTAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (76-126)

• Helix-hairpin-helix motif (HHH_3) (151-214)

3D structure

• AlphaFold DB: A0A4J1WRN7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA/celA/cilE	Streptococcus pneumoniae Rx1	99.537	100	0.995
comEA/celA/cilE	Streptococcus pneumoniae D39	99.537	100	0.995
comEA/celA/cilE	Streptococcus pneumoniae R6	99.537	100	0.995
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	96.759	100	0.968
comEA/celA/cilE	Streptococcus mitis NCTC 12261	95.833	100	0.958
comEA/celA/cilE	Streptococcus mitis SK321	89.815	100	0.898
comEA	Lactococcus lactis subsp. cremoris KW2	43.172	100	0.454
comEA	Bacillus subtilis subsp. subtilis str. 168	41.579	87.963	0.366
comEA	Latilactobacillus sakei subsp. sakei 23K	33.476	100	0.361