Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AAFF19_RS05980 Genome accession   NZ_CP152407
Coordinates   1290242..1291981 (-) Length   579 a.a.
NCBI ID   WP_182118245.1    Uniprot ID   -
Organism   Acidovorax sp. FHTAMBA     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1285242..1296981
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAFF19_RS05950 (AAFF19_05950) - 1285824..1286294 (+) 471 WP_182118241.1 NUDIX domain-containing protein -
  AAFF19_RS05955 (AAFF19_05955) - 1286391..1286609 (-) 219 WP_008905152.1 DNA gyrase inhibitor YacG -
  AAFF19_RS05960 (AAFF19_05960) zapD 1286660..1287415 (-) 756 WP_182118242.1 cell division protein ZapD -
  AAFF19_RS05965 (AAFF19_05965) coaE 1287501..1288121 (-) 621 WP_182118243.1 dephospho-CoA kinase -
  AAFF19_RS05970 (AAFF19_05970) - 1288125..1289006 (-) 882 WP_182118244.1 A24 family peptidase -
  AAFF19_RS05975 (AAFF19_05975) pilC 1289006..1290223 (-) 1218 WP_008905148.1 type II secretion system F family protein Machinery gene
  AAFF19_RS05980 (AAFF19_05980) pilB 1290242..1291981 (-) 1740 WP_182118245.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AAFF19_RS05990 (AAFF19_05990) - 1292228..1293157 (-) 930 WP_182118386.1 polyprenyl synthetase family protein -
  AAFF19_RS05995 (AAFF19_05995) rplU 1293383..1293694 (+) 312 WP_005794184.1 50S ribosomal protein L21 -
  AAFF19_RS06000 (AAFF19_06000) rpmA 1293710..1293967 (+) 258 WP_008905145.1 50S ribosomal protein L27 -
  AAFF19_RS06005 (AAFF19_06005) cgtA 1294139..1295209 (+) 1071 WP_182118246.1 Obg family GTPase CgtA -
  AAFF19_RS06010 (AAFF19_06010) proB 1295301..1296440 (+) 1140 WP_182118247.1 glutamate 5-kinase -

Sequence

Protein


Download         Length: 579 a.a.        Molecular weight: 62962.17 Da        Isoelectric Point: 5.1675

>NTDB_id=988918 AAFF19_RS05980 WP_182118245.1 1290242..1291981(-) (pilB) [Acidovorax sp. FHTAMBA]
MAAVDTAPKEASAVALPGLGRALMSAGKLTQKAAEDIYKKSQISRTSFIAELTGSGAVSAADLAHTVSAVFGAPLLDLEA
IDPLRLPKELLDGKICQAYKVVVLSKRNNRLIVATADPTDQEAAEKIKFTTQMGVDWIIAEYDKLCKLVESTTKSTNESM
ETLVSGGGDFEFDDAPVEETPDNNDAGATDVEDAPIVKFLHKMLLDAFNMRASDLHFEPYEHQYRVRFRIDGELREIASP
PIAIKDKLASRIKVISRLDISEKRVPQDGRMKLKVGPDRVIDFRVSTLPTLFGEKIVIRILDPSSAKLGIDALGYEPEEK
ERLLEAIGRPYGMILVTGPTGSGKTVSLYTCLNLLNKPGVNIATAEDPSEINLPGVNQVNVNEKAGLTFAVALKSFLRQD
PDIIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCPACKAPADI
PHESLLEAGYKEEDIDGSWVTYRPVGCSACNNGYKGRVGIYQVMPVSEDIQRIILRDGSALEIAEQARAEGVRSLRESGL
HKARLGFTSLEEVLAVTNE

Nucleotide


Download         Length: 1740 bp        

>NTDB_id=988918 AAFF19_RS05980 WP_182118245.1 1290242..1291981(-) (pilB) [Acidovorax sp. FHTAMBA]
ATGGCCGCTGTCGATACTGCCCCCAAAGAAGCCTCCGCAGTGGCCCTCCCCGGACTGGGCAGGGCCCTGATGTCTGCCGG
CAAGCTGACCCAAAAGGCAGCGGAAGACATTTACAAAAAATCACAAATTAGCCGCACCAGCTTTATCGCAGAATTGACGG
GATCCGGTGCGGTATCAGCGGCAGACTTGGCCCACACGGTTTCAGCCGTGTTTGGCGCTCCTTTGCTGGATCTGGAAGCG
ATTGATCCACTGCGGCTGCCCAAAGAACTTCTGGATGGCAAAATTTGCCAAGCCTACAAGGTAGTTGTTTTAAGCAAGCG
CAATAATCGCCTTATTGTTGCCACGGCAGATCCTACCGATCAGGAAGCAGCAGAGAAAATCAAATTCACGACCCAGATGG
GGGTGGATTGGATTATTGCAGAGTATGACAAACTGTGTAAATTGGTCGAAAGTACGACCAAGTCTACCAACGAGTCCATG
GAAACGTTGGTCAGTGGTGGGGGCGATTTTGAATTTGACGACGCCCCCGTTGAAGAGACGCCGGACAACAATGATGCGGG
CGCCACAGACGTCGAAGACGCTCCAATCGTCAAATTCCTGCACAAGATGCTGCTGGACGCCTTCAATATGCGCGCTTCAG
ATTTGCATTTTGAGCCTTACGAACACCAGTATCGCGTTCGATTTCGGATTGACGGAGAACTCCGGGAAATCGCATCGCCT
CCGATTGCCATCAAGGACAAGCTGGCGTCCCGCATCAAGGTGATTTCACGTCTGGATATTTCTGAAAAGCGCGTTCCCCA
AGACGGCCGGATGAAACTGAAGGTGGGTCCGGACAGGGTGATCGACTTCCGGGTGAGCACACTGCCGACCTTGTTCGGGG
AAAAAATCGTTATCCGTATTCTTGATCCGAGTAGCGCCAAACTGGGTATTGATGCTCTCGGATATGAACCCGAAGAAAAA
GAGCGACTGCTTGAAGCCATTGGACGCCCCTACGGGATGATTCTGGTAACCGGACCTACCGGCTCCGGTAAGACCGTATC
GTTGTACACCTGCCTGAACCTCCTGAACAAGCCAGGTGTCAATATTGCAACCGCAGAGGATCCTTCCGAAATCAACCTGC
CAGGCGTTAACCAAGTGAACGTTAACGAAAAAGCGGGCCTGACCTTTGCAGTTGCACTGAAGTCCTTTCTCCGCCAGGAT
CCGGACATTATCATGGTGGGGGAAATTCGCGATCTGGAAACGGCCGATATCTCCATCAAGGCGGCGCAGACAGGCCATTT
GGTATTGTCCACACTGCACACCAATGATGCCCCGACGACGCTGACGCGCATGCGCAATATGGGTATTGCGCCCTTTAACA
TCGCCTCCAGCGTGATCCTCATCACCGCCCAGCGCCTGGCCCGCCGACTGTGCCCAGCCTGCAAGGCACCCGCAGATATC
CCTCACGAGTCACTCCTGGAAGCAGGGTACAAGGAAGAGGACATTGACGGGAGTTGGGTCACCTATCGGCCTGTCGGTTG
TTCAGCCTGCAATAACGGATACAAAGGTCGGGTAGGCATTTACCAAGTGATGCCAGTTTCGGAAGACATTCAGCGCATCA
TTTTGCGCGATGGCAGTGCACTGGAAATTGCGGAACAGGCGCGCGCAGAGGGGGTCCGATCACTGCGCGAATCAGGTTTG
CACAAGGCGAGACTCGGCTTTACTTCACTGGAAGAAGTGCTGGCCGTCACCAACGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(202-469)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(54-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

53.701

100

0.539
  pilB Acinetobacter baumannii D1279779

52.901

100

0.535
  pilF Neisseria gonorrhoeae MS11

52.92

97.582

0.516
  pilB Legionella pneumophila strain ERS1305867

49.113

97.409

0.478
  pilB Vibrio parahaemolyticus RIMD 2210633

45.439

98.446

0.447
  pilB Vibrio campbellii strain DS40M4

44.858

97.409

0.437
  pilB Vibrio cholerae strain A1552

47.399

89.637

0.425
  pilF Thermus thermophilus HB27

39.33

97.927

0.385
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.977

97.927

0.382

Multiple sequence alignment