Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   AAFF19_RS00470 Genome accession   NZ_CP152407
Coordinates   108070..108579 (-) Length   169 a.a.
NCBI ID   WP_342721048.1    Uniprot ID   -
Organism   Acidovorax sp. FHTAMBA     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 103070..113579
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAFF19_RS00440 (AAFF19_00440) - 103398..104126 (+) 729 WP_008903831.1 TerC family protein -
  AAFF19_RS00445 (AAFF19_00445) - 104245..104697 (+) 453 WP_342721044.1 hypothetical protein -
  AAFF19_RS00450 (AAFF19_00450) - 104764..105117 (+) 354 WP_342721045.1 phage holin family protein -
  AAFF19_RS00455 (AAFF19_00455) - 105085..106593 (-) 1509 WP_342721805.1 M48 family metalloprotease -
  AAFF19_RS00460 (AAFF19_00460) moaC 106751..107251 (+) 501 WP_342721046.1 cyclic pyranopterin monophosphate synthase MoaC -
  AAFF19_RS00465 (AAFF19_00465) tfpZ 107248..108024 (-) 777 WP_342721047.1 TfpX/TfpZ family type IV pilin accessory protein -
  AAFF19_RS00470 (AAFF19_00470) pilA 108070..108579 (-) 510 WP_342721048.1 pilin Machinery gene
  AAFF19_RS00475 (AAFF19_00475) - 108787..109578 (-) 792 WP_342721049.1 3',5'-cyclic-nucleotide phosphodiesterase -
  AAFF19_RS00480 (AAFF19_00480) - 109591..111748 (-) 2158 Protein_90 adenylate/guanylate cyclase domain-containing protein -
  AAFF19_RS00485 (AAFF19_00485) - 111835..112491 (-) 657 WP_034693286.1 FHA domain-containing protein -
  AAFF19_RS00490 (AAFF19_00490) - 112536..113324 (-) 789 WP_085942508.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -

Sequence

Protein


Download         Length: 169 a.a.        Molecular weight: 17001.66 Da        Isoelectric Point: 9.5798

>NTDB_id=988909 AAFF19_RS00470 WP_342721048.1 108070..108579(-) (pilA) [Acidovorax sp. FHTAMBA]
MKRTLQQGFTLIELMIVVAIIGILAAVALPAYQDYTVRARVTEGLVAASAAKINVADVLSSGNPNASAQGYNLGFTSPTA
SRNLTSVTIAPATGLITVTTTANAGNGTLTLMPYLGAALPGTALPVGTATFTPPADSAKWRCAAVGATPLAVGQTAGTLP
ARFAPAECK

Nucleotide


Download         Length: 510 bp        

>NTDB_id=988909 AAFF19_RS00470 WP_342721048.1 108070..108579(-) (pilA) [Acidovorax sp. FHTAMBA]
ATGAAGCGTACCCTGCAACAAGGTTTTACCCTGATCGAACTGATGATCGTGGTGGCGATTATTGGCATCTTGGCAGCTGT
GGCTCTACCTGCCTACCAAGACTACACAGTGCGTGCTCGCGTCACTGAAGGTCTCGTAGCCGCTTCTGCGGCCAAAATTA
ACGTTGCGGATGTTTTGTCTTCTGGCAACCCGAATGCATCTGCTCAAGGTTACAACTTGGGCTTTACTTCGCCAACCGCT
TCGCGGAATTTGACTAGCGTAACTATTGCTCCTGCTACTGGTTTGATTACCGTTACCACCACAGCTAACGCAGGCAACGG
TACACTGACCCTCATGCCTTATTTGGGCGCCGCTCTGCCAGGTACCGCATTGCCTGTTGGTACAGCAACCTTTACACCAC
CAGCAGATTCCGCTAAGTGGCGTTGTGCTGCTGTCGGTGCAACACCGTTGGCGGTTGGTCAAACCGCTGGTACGCTTCCG
GCTCGGTTTGCCCCTGCCGAGTGCAAATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(37-169)

Prokaryotic N-terminal methylation motif (N_methyl)

(6-29)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

46.591

100

0.485
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

37.563

100

0.438
  pilA2 Legionella pneumophila strain ERS1305867

44.512

97.041

0.432
  pilA2 Legionella pneumophila str. Paris

44.512

97.041

0.432
  comP Acinetobacter baylyi ADP1

37.143

100

0.385
  pilA/pilAI Pseudomonas stutzeri DSM 10701

35.673

100

0.361

Multiple sequence alignment