Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   AAGQ88_RS24705 Genome accession   NZ_CP152388
Coordinates   5541762..5543837 (-) Length   691 a.a.
NCBI ID   WP_342653869.1    Uniprot ID   -
Organism   Pseudomonas sp. F3-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5536762..5548837
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAGQ88_RS24690 (AAGQ88_24690) - 5538372..5539994 (-) 1623 WP_093465339.1 AAA family ATPase -
  AAGQ88_RS24695 (AAGQ88_24695) aroB 5540005..5541108 (-) 1104 WP_342653868.1 3-dehydroquinate synthase -
  AAGQ88_RS24700 (AAGQ88_24700) aroK 5541239..5541757 (-) 519 WP_093465345.1 shikimate kinase AroK -
  AAGQ88_RS24705 (AAGQ88_24705) pilQ 5541762..5543837 (-) 2076 WP_342653869.1 type IV pilus secretin PilQ Machinery gene
  AAGQ88_RS24710 (AAGQ88_24710) - 5543891..5544418 (-) 528 WP_093465348.1 pilus assembly protein PilP -
  AAGQ88_RS24715 (AAGQ88_24715) pilO 5544415..5545062 (-) 648 WP_285422690.1 type 4a pilus biogenesis protein PilO -
  AAGQ88_RS24720 (AAGQ88_24720) - 5545059..5545625 (-) 567 WP_285422691.1 PilN domain-containing protein -
  AAGQ88_RS24725 (AAGQ88_24725) pilM 5545625..5546689 (-) 1065 WP_093465357.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 691 a.a.        Molecular weight: 74475.65 Da        Isoelectric Point: 5.2841

>NTDB_id=988847 AAGQ88_RS24705 WP_342653869.1 5541762..5543837(-) (pilQ) [Pseudomonas sp. F3-2]
MTRILSIIGVSLWMAMLSPILQAANLKTLDVAALPGDRVELKLSFDGPVAAPRGYTTEQPARIALDLPGVTSQLAIKSRD
LGSGNAHSVVVVEAKDRTRVIINLTTLAPYSSRVDGNNLFVVVGQGAASAQASQPSTAQGAPRVSVPPAAPKVAARAYAP
GPRAVKNVDFQRGELGEGNVIIELSDTGIAPDIQEQGGKIRVDFAKTQLPDPLRVRLDVKDFATPVQFVNSSVTGDKTSI
SIEPSGAFDYSAYQTENKLTISVRPLTNEDQERRNAEKLVYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGI
TLRLQNVPWDQALDLVLKTKGLDKRKIGNVLLVGPADEIAARERQELESLKQISELAPLRRELLQVNYAKAADIAKLFQS
VTSAESKSDERGSITVDERTNNIIAYQTQDRLDELRRIVSQLDIPVRQVMIEARIVEANVDYDKALGVRWGGSSTHGNFT
AGGNGTVTGAGNTNGPYVDMGVSNPTSTIGLGFLTNNTILDLELSAMEKTGNGEVVSQPKVVTSDKETAKILKGTEVPYQ
EASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYVNTVLGVPPIKKNEVNAKVLVADGETIVIGGVFSNT
QSKVVDKVPFLGDVPYVGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2076 bp        

>NTDB_id=988847 AAGQ88_RS24705 WP_342653869.1 5541762..5543837(-) (pilQ) [Pseudomonas sp. F3-2]
ATGACTAGGATTCTTTCGATTATCGGCGTATCGCTATGGATGGCGATGCTTTCCCCGATTCTCCAGGCGGCGAACCTCAA
GACCCTGGATGTCGCTGCGTTGCCAGGGGACCGGGTCGAATTGAAACTGTCATTCGACGGCCCGGTTGCCGCGCCGCGTG
GCTACACCACCGAACAGCCAGCGCGCATTGCGCTGGACCTGCCAGGCGTGACCAGCCAGTTGGCGATCAAGAGTCGTGAT
CTGGGCTCGGGCAATGCTCACAGCGTTGTTGTGGTCGAAGCCAAGGATCGCACGCGGGTCATCATCAACCTGACCACGCT
TGCGCCTTACAGCTCTCGGGTCGATGGTAACAATCTGTTTGTCGTCGTCGGCCAAGGCGCGGCTTCGGCACAAGCTTCGC
AGCCAAGCACTGCCCAAGGTGCGCCGCGAGTCAGCGTTCCGCCTGCTGCTCCGAAGGTTGCTGCGCGCGCCTATGCGCCG
GGCCCCAGAGCGGTCAAAAACGTTGACTTCCAGCGCGGTGAACTGGGCGAAGGCAACGTCATCATCGAGCTGAGCGACAC
TGGCATTGCGCCGGACATCCAGGAGCAAGGCGGCAAGATTCGTGTCGACTTCGCCAAGACCCAATTGCCCGATCCCCTGC
GCGTGCGTCTGGACGTCAAGGACTTCGCCACGCCGGTGCAATTCGTCAACTCAAGTGTCACCGGCGACAAGACGAGCATT
TCCATCGAGCCGTCCGGCGCGTTCGATTATTCCGCCTATCAGACCGAGAACAAGCTGACCATCAGCGTGCGCCCACTGAC
CAATGAGGATCAGGAGCGTCGTAACGCGGAGAAACTGGTGTACACCGGCGAGAAGTTGTCGCTCAACTTCCAGGACATCG
ACGTTCGCTCGGTGCTGCAGCTGATCGCTGATTTCACCAACCTCAACCTCGTCGCCAGCGACACCGTTCAGGGCGGCATC
ACGCTGCGCCTGCAAAACGTGCCGTGGGATCAGGCGCTGGACCTGGTGCTCAAGACCAAAGGCCTGGACAAGCGCAAGAT
CGGCAACGTCTTGCTTGTCGGCCCGGCTGACGAAATCGCCGCCCGCGAACGTCAGGAACTGGAGTCGCTCAAGCAGATTT
CCGAGCTGGCGCCGTTGCGTCGTGAGTTGCTGCAAGTCAACTACGCCAAGGCCGCCGACATCGCCAAGCTGTTCCAGTCG
GTGACCAGCGCGGAGTCCAAGTCTGACGAGCGCGGTTCGATCACGGTCGACGAGCGTACCAACAACATCATCGCCTACCA
GACGCAGGACCGTCTGGACGAGCTGCGCCGCATCGTTTCGCAACTGGATATCCCGGTCCGTCAGGTCATGATCGAGGCGC
GCATCGTCGAGGCCAACGTTGACTACGACAAAGCACTGGGCGTGCGCTGGGGCGGGTCAAGTACCCATGGCAACTTCACC
GCTGGCGGGAACGGCACCGTCACCGGCGCCGGCAACACCAACGGGCCCTATGTGGACATGGGCGTGAGCAACCCGACGTC
GACGATCGGCTTGGGCTTCCTGACTAACAACACCATTCTTGACCTTGAACTGAGCGCGATGGAAAAGACCGGTAACGGTG
AAGTGGTCTCCCAGCCAAAGGTTGTCACCTCGGACAAGGAAACCGCGAAAATCCTGAAAGGTACGGAAGTGCCGTACCAG
GAAGCCAGCTCCAGCGGTGCCACGTCGGTCTCCTTCAAAGAGGCCTCGCTCTCGCTGGAAGTGACGCCGCAGATCACGCC
GGACAACCGCATCATCATGGAAGTGAAAGTCACCAAGGATGAGCCTGACTACGTCAATACCGTGCTCGGCGTGCCGCCGA
TCAAGAAGAACGAGGTCAATGCCAAGGTGCTGGTCGCGGACGGCGAGACCATTGTAATTGGTGGTGTGTTCTCGAATACG
CAGAGTAAAGTCGTCGACAAGGTGCCATTTTTGGGCGATGTGCCGTATGTTGGCCGCCTTTTCCGGCGGGATGTGGTCTC
GGAGAAAAAATCCGAGCTGTTGGTGTTCCTCACTCCGCGTATCATGAACAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(383-448)

Bacterial type II and III secretion system protein (Secretin)

(526-682)

AMIN domain (AMIN)

(38-122)

Secretin and TonB N terminus short domain (STN)

(309-355)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.006

100

0.748
  comQ Acinetobacter baylyi ADP1

42.507

100

0.427
  pilQ Acinetobacter baumannii strain A118

39.674

100

0.423
  pilQ Acinetobacter baumannii D1279779

39.674

100

0.423
  pilQ Legionella pneumophila strain ERS1305867

39.586

97.974

0.388
  pilQ Legionella pneumophila strain Lp02

39.291

97.974

0.385

Multiple sequence alignment