Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   AAGQ88_RS24725 Genome accession   NZ_CP152388
Coordinates   5545625..5546689 (-) Length   354 a.a.
NCBI ID   WP_093465357.1    Uniprot ID   -
Organism   Pseudomonas sp. F3-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5540625..5551689
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAGQ88_RS24700 (AAGQ88_24700) aroK 5541239..5541757 (-) 519 WP_093465345.1 shikimate kinase AroK -
  AAGQ88_RS24705 (AAGQ88_24705) pilQ 5541762..5543837 (-) 2076 WP_342653869.1 type IV pilus secretin PilQ Machinery gene
  AAGQ88_RS24710 (AAGQ88_24710) - 5543891..5544418 (-) 528 WP_093465348.1 pilus assembly protein PilP -
  AAGQ88_RS24715 (AAGQ88_24715) pilO 5544415..5545062 (-) 648 WP_285422690.1 type 4a pilus biogenesis protein PilO -
  AAGQ88_RS24720 (AAGQ88_24720) - 5545059..5545625 (-) 567 WP_285422691.1 PilN domain-containing protein -
  AAGQ88_RS24725 (AAGQ88_24725) pilM 5545625..5546689 (-) 1065 WP_093465357.1 pilus assembly protein PilM Machinery gene
  AAGQ88_RS24730 (AAGQ88_24730) - 5546896..5549373 (+) 2478 WP_285422693.1 penicillin-binding protein 1A -
  AAGQ88_RS24735 (AAGQ88_24735) - 5549538..5550806 (-) 1269 WP_093465362.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38145.73 Da        Isoelectric Point: 4.6363

>NTDB_id=988848 AAGQ88_RS24725 WP_093465357.1 5545625..5546689(-) (pilM) [Pseudomonas sp. F3-2]
MFELFSKKANTLLGIDISSTSVKLLELSRSGTRYKVESYAVEPLPANAVVEKNIAELEGVGNALTRVLVKAKSNVKIAAV
AVAGSAVITKTIEMDAGLSDDEMENQLKIEADQYIPYPLEEVAIDFEVQGYSARNPERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLSAQLGQDHEQLTVAVIDIGATMTTLSVLHNGRIIYTREQLFGGRQLTEEIQRRYGLSV
GEAGLAKKQGGLPDDYVLEVLQPFKDAVVQQVSRSLQFFFAAGQYNRVDYIMLAGGTASIAGLDRLIQERLGTPTMVANP
FADMALSAKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=988848 AAGQ88_RS24725 WP_093465357.1 5545625..5546689(-) (pilM) [Pseudomonas sp. F3-2]
GTGTTCGAACTCTTCAGTAAGAAGGCCAATACCCTTCTAGGGATCGATATAAGCTCCACCTCGGTAAAGCTCCTTGAATT
GAGTCGTTCCGGTACCCGTTACAAGGTTGAGTCTTACGCGGTGGAGCCGCTGCCGGCAAACGCCGTGGTCGAGAAAAACA
TTGCCGAGCTCGAGGGCGTTGGCAATGCGTTGACGCGTGTGCTCGTCAAAGCCAAGAGCAACGTCAAAATTGCGGCGGTT
GCCGTAGCCGGATCGGCGGTGATTACCAAGACCATCGAGATGGACGCCGGCCTGTCCGACGACGAGATGGAAAACCAGCT
CAAAATCGAAGCCGATCAATACATTCCCTATCCGCTGGAAGAAGTCGCCATCGATTTCGAAGTCCAGGGCTATTCGGCGC
GTAATCCAGAGCGTGTCGAGGTGCTGCTGGCAGCGTGCCGCAAGGAAAACGTCGAGGTTCGCGAAGCTGCGCTTGCACTG
GCAGGCCTGACCGCACGTGTCGTCGACGTCGAGGCCTATGCGCTGGAGCGCTCGTTTGGCCTGCTCTCCGCGCAACTGGG
TCAGGATCACGAGCAATTGACCGTCGCCGTCATTGATATCGGCGCGACGATGACCACCCTCAGCGTTCTGCACAATGGCC
GCATCATCTATACCCGCGAGCAACTGTTCGGCGGCCGTCAGCTGACCGAAGAAATCCAGCGCCGTTATGGCTTATCGGTC
GGGGAAGCCGGGCTTGCGAAAAAACAGGGCGGTCTGCCCGACGACTATGTGCTGGAGGTGCTGCAGCCGTTCAAGGATGC
AGTGGTTCAGCAGGTTTCCCGATCTCTGCAGTTCTTCTTCGCCGCCGGGCAATATAACCGTGTCGACTACATCATGCTGG
CGGGCGGGACGGCCTCCATTGCCGGACTGGATCGACTGATCCAGGAACGCCTCGGTACGCCGACCATGGTCGCCAACCCC
TTCGCCGACATGGCTCTGAGCGCCAAAGTCAACGCTGGCGCGCTCGCCAGTGATGCGCCGGCGCTGATGATTGCGTGCGG
TCTGGCGTTGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.215

100

0.562
  comM Acinetobacter nosocomialis M2

56.215

100

0.562
  comM Acinetobacter baylyi ADP1

53.672

100

0.537
  pilM Legionella pneumophila strain ERS1305867

46.61

100

0.466

Multiple sequence alignment