Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AAGQ88_RS23140 Genome accession   NZ_CP152388
Coordinates   5170693..5172387 (+) Length   564 a.a.
NCBI ID   WP_342653692.1    Uniprot ID   -
Organism   Pseudomonas sp. F3-2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5165693..5177387
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAGQ88_RS23115 (AAGQ88_23115) - 5166004..5166375 (-) 372 WP_342653688.1 hypothetical protein -
  AAGQ88_RS23120 (AAGQ88_23120) - 5167360..5167719 (-) 360 WP_342653689.1 hypothetical protein -
  AAGQ88_RS23130 (AAGQ88_23130) - 5168540..5169922 (-) 1383 WP_342653690.1 O-antigen ligase family protein -
  AAGQ88_RS23135 (AAGQ88_23135) - 5170035..5170466 (-) 432 WP_342653691.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  AAGQ88_RS23140 (AAGQ88_23140) pilB 5170693..5172387 (+) 1695 WP_342653692.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AAGQ88_RS23145 (AAGQ88_23145) pilC 5172390..5173607 (+) 1218 WP_342653693.1 type II secretion system F family protein Machinery gene
  AAGQ88_RS23150 (AAGQ88_23150) pilD 5173609..5174481 (+) 873 WP_342653694.1 A24 family peptidase Machinery gene
  AAGQ88_RS23155 (AAGQ88_23155) coaE 5174481..5175110 (+) 630 WP_342655871.1 dephospho-CoA kinase -
  AAGQ88_RS23160 (AAGQ88_23160) yacG 5175110..5175313 (+) 204 WP_062378727.1 DNA gyrase inhibitor YacG -
  AAGQ88_RS23165 (AAGQ88_23165) - 5175383..5176069 (-) 687 WP_342653695.1 energy-coupling factor ABC transporter permease -
  AAGQ88_RS23170 (AAGQ88_23170) - 5176231..5176875 (-) 645 WP_342653696.1 hydrolase -

Sequence

Protein


Download         Length: 564 a.a.        Molecular weight: 62245.49 Da        Isoelectric Point: 5.8603

>NTDB_id=988842 AAGQ88_RS23140 WP_342653692.1 5170693..5172387(+) (pilB) [Pseudomonas sp. F3-2]
MTDVVLTGLAKQLVVAELLNEKSAQQAYQQARRDKVSLVSYLVQNKLVKSLTLAEVASDQFGIPFLDLNTLDKESQPKGL
VSEKLVRQHSALPLWRRGNKLFIGVSDPTNHQAITDIQFSTGLNTEAILVEDDKLTLAIEKFFDTHSGMGDLADVDLGLE
VEAVDESKETSIGGQDADDAPVVRFVNKMLMDAIRLGSSDLHFEPYEKIFRVRLRTDGILHEVAKPPTHLAGRIAARLKV
MASLDISERRKPQDGRIKLRISKNRAIDFRVNTLPTLWGEKIVMRILDPTSAQMGIDALGYEPEQKALYLEALSKPQGMI
LVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFAQALRAFLRQDPDVIMVGEIRDLET
AEIAIKASQTGHMVLSTLHTNSAAETLTRLHHMGVAAFNIATAINLIIAQRLARKLCPHCKKEVDIPRETLIAEGFPEEK
IGAFKLYSPVGCEQCNAGYKGRVGIYEVVKSTPALERIIMEEGNSIEISEQMRKDGFNDLRTSGLMKAMQGVTSLEEVNR
VTKD

Nucleotide


Download         Length: 1695 bp        

>NTDB_id=988842 AAGQ88_RS23140 WP_342653692.1 5170693..5172387(+) (pilB) [Pseudomonas sp. F3-2]
ATGACTGATGTCGTGCTTACGGGTTTGGCCAAACAGCTTGTCGTGGCTGAGCTCTTGAATGAAAAATCTGCGCAGCAGGC
GTATCAGCAGGCTCGCCGCGACAAGGTGTCGCTGGTCAGCTATCTGGTACAGAACAAGCTGGTAAAAAGTCTGACCCTTG
CCGAAGTAGCTTCCGACCAGTTCGGCATTCCCTTTCTTGACCTCAATACCCTCGATAAGGAAAGCCAGCCCAAGGGGTTG
GTCAGCGAAAAGCTCGTGCGCCAGCACTCGGCGCTGCCTTTATGGCGACGGGGTAACAAGCTGTTTATTGGTGTATCGGA
CCCGACCAATCACCAGGCCATTACCGACATCCAGTTCAGCACCGGCTTGAATACAGAAGCGATTCTGGTCGAAGACGACA
AGCTCACTCTTGCCATCGAGAAGTTCTTCGACACCCACAGTGGCATGGGCGATCTGGCCGATGTTGACCTGGGGTTGGAA
GTCGAAGCTGTAGACGAAAGCAAAGAGACCTCCATTGGCGGTCAGGATGCCGACGACGCGCCGGTGGTCCGGTTCGTGAA
CAAGATGCTGATGGACGCGATCCGTCTCGGCTCTTCGGACCTGCATTTTGAGCCTTACGAGAAGATCTTCCGGGTTCGCC
TGCGCACTGACGGCATCCTTCATGAAGTGGCCAAACCGCCGACGCACCTGGCCGGCCGTATCGCTGCGCGCTTGAAGGTC
ATGGCCAGTCTGGATATTTCCGAGCGCCGCAAGCCGCAGGACGGGCGCATCAAGCTGCGCATTTCGAAAAACCGCGCCAT
CGACTTTCGTGTCAACACGTTGCCGACCTTGTGGGGCGAGAAGATCGTGATGCGGATTCTCGACCCGACCAGCGCGCAGA
TGGGCATCGACGCGCTGGGTTACGAGCCGGAGCAGAAAGCGCTGTATCTCGAAGCGCTCAGCAAACCACAGGGCATGATT
CTGGTCACCGGTCCTACAGGCTCGGGTAAAACCGTTTCGCTCTATACCGGTTTGAATATTCTCAACACAACGGACATCAA
CATCTCCACCGCCGAGGATCCGGTCGAAATCAACCTGGAAGGCATCAACCAGGTCAACGTCAACCCGCGTCAGGGCATGG
ACTTCGCCCAGGCGCTGCGGGCATTTCTGCGTCAGGACCCGGACGTGATCATGGTCGGTGAGATTCGTGACCTCGAAACC
GCCGAGATCGCGATCAAGGCCTCGCAGACCGGCCACATGGTGCTGTCGACGCTGCACACCAACAGCGCGGCGGAAACACT
GACTCGCCTGCACCACATGGGCGTGGCGGCTTTCAACATTGCTACCGCGATCAACCTGATCATCGCTCAGCGGCTGGCCC
GCAAGCTCTGCCCGCACTGCAAGAAAGAAGTCGACATCCCGCGCGAGACATTGATTGCCGAAGGTTTCCCGGAAGAGAAA
ATCGGCGCGTTCAAGCTTTACTCTCCAGTGGGCTGCGAACAATGCAACGCCGGCTACAAAGGCCGTGTCGGTATTTACGA
AGTGGTGAAGAGCACTCCCGCCCTGGAGCGCATCATCATGGAGGAAGGCAACTCCATCGAGATTTCCGAACAGATGCGCA
AGGACGGCTTCAACGACCTGCGCACCTCCGGCCTGATGAAGGCCATGCAAGGCGTGACCAGCCTTGAGGAAGTCAACCGG
GTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.979

100

0.58
  pilB Acinetobacter baylyi ADP1

57.904

99.823

0.578
  pilB Legionella pneumophila strain ERS1305867

54.77

100

0.55
  pilB Vibrio cholerae strain A1552

50.267

99.468

0.5
  pilB Vibrio campbellii strain DS40M4

49.911

99.823

0.498
  pilB Vibrio parahaemolyticus RIMD 2210633

50.727

97.518

0.495
  pilF Neisseria gonorrhoeae MS11

48.663

99.468

0.484
  pilF Thermus thermophilus HB27

39.029

98.582

0.385
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.885

92.908

0.371

Multiple sequence alignment