Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   AAGT77_RS03385 Genome accession   NZ_CP152380
Coordinates   734203..736317 (+) Length   704 a.a.
NCBI ID   WP_138438138.1    Uniprot ID   -
Organism   Marinobacter alkaliphilus strain SBY-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 729203..741317
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAGT77_RS03365 (AAGT77_03365) pilM 731401..732465 (+) 1065 WP_342631840.1 pilus assembly protein PilM Machinery gene
  AAGT77_RS03370 (AAGT77_03370) - 732465..733028 (+) 564 WP_072678329.1 PilN domain-containing protein -
  AAGT77_RS03375 (AAGT77_03375) - 733028..733639 (+) 612 WP_342631841.1 type 4a pilus biogenesis protein PilO -
  AAGT77_RS03380 (AAGT77_03380) - 733642..734184 (+) 543 WP_342631842.1 pilus assembly protein PilP -
  AAGT77_RS03385 (AAGT77_03385) pilQ 734203..736317 (+) 2115 WP_138438138.1 type IV pilus secretin PilQ Machinery gene
  AAGT77_RS03390 (AAGT77_03390) aroK 736441..737025 (+) 585 WP_342631843.1 shikimate kinase AroK -
  AAGT77_RS03395 (AAGT77_03395) aroB 737000..738088 (+) 1089 WP_342631844.1 3-dehydroquinate synthase -
  AAGT77_RS03400 (AAGT77_03400) - 738132..739655 (+) 1524 WP_342631845.1 AAA family ATPase -

Sequence

Protein


Download         Length: 704 a.a.        Molecular weight: 75849.62 Da        Isoelectric Point: 4.5021

>NTDB_id=988760 AAGT77_RS03385 WP_138438138.1 734203..736317(+) (pilQ) [Marinobacter alkaliphilus strain SBY-1]
MNEKNMHEQNGLRSRLAMFKKLNVCVGVIAIGLWSSLASAVTLEDVSFSSLPGERLEVTMKFDGPPPEPTGYTIERPARI
AVDLKDTTSGLNQRSIPLGSGNAQSVTVVETKDRTRLIFNLVELVPHDTVRQNNSLVMTIGGASGAPTVTAGAQQGRTGT
TNASAANALAGVDFRRSKEGEGRVIVDLGSASAPVNLSELAGRIRLTMPGLEVPMDLRRRLDVTDFATPVTRIDTFIEDG
NAVVEIRPEGNYDYIAYQSGREFTVSVERISEEEAETRREEKFPYTGDKLSLNFQDIEVRSVLQLIADFTGLNLVASDTV
GGSITLRLQNVPWDQALDLILKTKGLDKRQIGNVLLVAPAEEIAAREKLELETSKQIAELAPVRLDIIQVNYAKAADVVA
LIRADEELISPRGFVSSDVRTNTISVRETQQKLEEIRRLVGTWDVPVRQVSIEARIVRAQTNVAENLGVRWGGAAYDVSG
NDVISIGGSLGTLQEARDAAAGGGGGISFPGALGVDLGVSGDGASSFAIGWGSDDFLIDLELSALETDGRAEVVSQPRVV
TADRQSASIKSGEEIPYQEATSSGATNVEFKEAVLSLEVTPQITPDDKIIMDLVVNQDSRGEVTAGIPSINTNSVTTQVL
VANGETVVLGGIFQSEVATQTTKTPFLGDIPYLGRLFKRTEHIDERSELLIFITPKIIKNDLIQ

Nucleotide


Download         Length: 2115 bp        

>NTDB_id=988760 AAGT77_RS03385 WP_138438138.1 734203..736317(+) (pilQ) [Marinobacter alkaliphilus strain SBY-1]
ATGAACGAAAAAAACATGCACGAACAAAATGGTTTAAGGTCGAGGCTAGCGATGTTCAAAAAACTCAATGTCTGCGTGGG
CGTGATCGCCATCGGGTTATGGAGCAGCCTGGCCAGCGCGGTCACGCTGGAAGACGTGTCGTTTTCGTCGCTGCCGGGAG
AGCGTCTGGAAGTGACCATGAAATTCGACGGGCCACCGCCGGAACCAACGGGTTACACGATTGAGCGTCCGGCGCGCATC
GCTGTGGACCTCAAAGATACCACCAGTGGGCTAAACCAGCGGAGCATCCCGCTTGGATCTGGCAACGCCCAGAGCGTGAC
TGTGGTGGAAACCAAAGATCGCACACGGCTGATCTTTAATCTGGTCGAGCTGGTGCCGCACGATACCGTTCGCCAGAACA
ATTCGCTGGTGATGACCATTGGTGGCGCCAGTGGCGCGCCGACGGTGACAGCAGGAGCCCAGCAGGGCAGGACCGGAACG
ACCAATGCCTCTGCAGCCAACGCCTTGGCTGGTGTTGACTTCCGTCGCAGCAAAGAGGGTGAAGGGCGCGTTATAGTTGA
TCTGGGTAGCGCGTCGGCTCCGGTGAACCTGTCTGAACTGGCCGGTCGTATTCGCCTGACCATGCCGGGCCTTGAAGTGC
CTATGGATCTCCGTCGTCGTCTTGACGTGACTGATTTTGCGACCCCTGTTACCCGCATTGATACCTTTATTGAAGATGGT
AACGCTGTTGTTGAGATTCGCCCGGAAGGAAACTACGACTACATAGCGTATCAGTCTGGCCGTGAATTCACCGTGAGCGT
GGAACGCATCTCGGAAGAAGAGGCAGAGACCCGTCGCGAAGAGAAATTCCCGTACACCGGTGACAAGCTGTCCTTGAACT
TCCAGGACATCGAGGTACGTTCGGTTCTCCAGTTGATTGCTGACTTTACCGGCCTGAATCTTGTGGCTAGTGACACCGTT
GGTGGCAGCATCACTCTGCGTTTGCAGAATGTACCCTGGGATCAAGCGCTTGATCTGATTTTGAAGACCAAAGGTCTGGA
CAAGCGTCAGATCGGCAACGTTCTGCTGGTGGCTCCGGCAGAGGAAATCGCCGCCCGCGAAAAGCTTGAGCTTGAGACCA
GTAAGCAGATTGCAGAACTGGCCCCGGTGCGCCTGGATATCATTCAGGTGAACTACGCGAAGGCGGCCGATGTTGTGGCG
CTGATTCGCGCGGATGAAGAGCTGATTTCACCCCGTGGCTTCGTATCTTCCGATGTTCGCACCAATACCATCAGTGTCCG
TGAGACCCAGCAGAAGCTGGAAGAGATTCGTCGCCTTGTCGGCACCTGGGATGTGCCTGTACGGCAGGTGTCCATCGAAG
CCCGAATTGTTCGCGCGCAGACCAATGTGGCTGAAAATCTCGGGGTTCGCTGGGGCGGCGCCGCTTACGATGTCAGTGGT
AATGATGTCATCAGTATCGGCGGATCGTTGGGTACCCTCCAGGAGGCCCGTGATGCCGCAGCCGGTGGTGGCGGTGGGAT
TTCGTTCCCCGGCGCTCTGGGAGTCGATCTGGGGGTTTCTGGCGACGGTGCCTCATCCTTTGCCATTGGCTGGGGTAGTG
ACGACTTCCTGATCGACCTTGAGCTTTCTGCTCTGGAAACAGACGGTCGCGCCGAGGTTGTCTCTCAGCCACGCGTGGTG
ACAGCAGATCGCCAGAGTGCTTCCATTAAGTCAGGTGAGGAGATTCCCTATCAGGAAGCGACCTCCAGTGGCGCGACTAA
CGTTGAGTTCAAGGAAGCGGTTCTTTCACTGGAGGTAACCCCGCAGATCACTCCGGATGACAAGATCATCATGGACTTGG
TGGTAAACCAGGATTCCCGGGGCGAGGTAACGGCTGGTATACCCTCTATCAACACCAACTCCGTGACCACGCAGGTCTTG
GTAGCCAACGGCGAAACTGTTGTATTGGGCGGTATCTTCCAGTCGGAAGTGGCTACACAAACCACCAAGACGCCGTTCCT
GGGGGATATTCCCTACCTCGGTCGCCTGTTCAAGCGCACGGAACACATCGATGAGCGCAGCGAATTGCTGATTTTCATCA
CACCCAAGATCATCAAGAACGACTTGATTCAGTAA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(313-359)

Bacterial type II/III secretion system short domain (Secretin_N)

(387-449)

AMIN domain (AMIN)

(56-137)

Bacterial type II and III secretion system protein (Secretin)

(544-699)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

47.686

100

0.483
  pilQ Acinetobacter baumannii D1279779

39.917

100

0.411
  pilQ Acinetobacter baumannii strain A118

39.917

100

0.411
  comQ Acinetobacter baylyi ADP1

40.453

100

0.406
  pilQ Legionella pneumophila strain ERS1305867

40.059

95.739

0.384
  pilQ Legionella pneumophila strain Lp02

39.911

95.739

0.382

Multiple sequence alignment