Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   AAG563_RS25595 Genome accession   NZ_CP152341
Coordinates   5670506..5672578 (+) Length   690 a.a.
NCBI ID   WP_009041823.1    Uniprot ID   -
Organism   Pseudomonas chlororaphis strain NCIMB_15121     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5665506..5677578
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAG563_RS25575 (AAG563_25575) pilM 5667736..5668788 (+) 1053 WP_037004437.1 pilus assembly protein PilM Machinery gene
  AAG563_RS25580 (AAG563_25580) - 5668788..5669354 (+) 567 WP_009041820.1 PilN domain-containing protein -
  AAG563_RS25585 (AAG563_25585) pilO 5669351..5669968 (+) 618 WP_009041821.1 type 4a pilus biogenesis protein PilO -
  AAG563_RS25590 (AAG563_25590) - 5669965..5670492 (+) 528 WP_009041822.1 pilus assembly protein PilP -
  AAG563_RS25595 (AAG563_25595) pilQ 5670506..5672578 (+) 2073 WP_009041823.1 type IV pilus secretin PilQ Machinery gene
  AAG563_RS25600 (AAG563_25600) aroK 5672583..5673101 (+) 519 WP_009041824.1 shikimate kinase AroK -
  AAG563_RS25605 (AAG563_25605) aroB 5673250..5674350 (+) 1101 WP_009041825.1 3-dehydroquinate synthase -
  AAG563_RS25610 (AAG563_25610) - 5674361..5675950 (+) 1590 WP_009041826.1 AAA family ATPase -

Sequence

Protein


Download         Length: 690 a.a.        Molecular weight: 74762.88 Da        Isoelectric Point: 5.2124

>NTDB_id=988481 AAG563_RS25595 WP_009041823.1 5670506..5672578(+) (pilQ) [Pseudomonas chlororaphis strain NCIMB_15121]
MNRIFSAFGIALWMALLSPMAMATSLKALDVAALPGDRLELKLTFDGPVPEPRGYTTDQPARIALDLPGVVSQLADKSRD
LGNGNARSVTVVESGQRTRVVVNLVALAPYSTRVAGNQLFVVIGQGATAALSAPGQPAVASGRAKAVASVGRSIRSVDFQ
RGEQGEGNVLIDLSDSEMSPDIQERDGKLVLTFAKARLPEPLRVRLDVKDFATPVQFVSARAESGKAIVTIEPGGTFDYS
TYQTDNKLTVSVRSMTSGDLQKRNAERSAYNGEKLSLNFQDIEVRSVLQLIADFTNLNLVASDTVQGGITLRLQNVPWDQ
ALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIADLEPLRRELLQVNYAKAAEIAKLFQSVTRAEDKSDER
GSITVDERTNNIIAYQTRERLDELRRIVSQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNQGNWNASGVSNGTNG
SSTIGTPGSTSSNSPFVDLGTTANTSGLGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPYQE
AASSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLINDGETIVIGGVFSNTQ
SKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2073 bp        

>NTDB_id=988481 AAG563_RS25595 WP_009041823.1 5670506..5672578(+) (pilQ) [Pseudomonas chlororaphis strain NCIMB_15121]
ATGAACAGGATCTTTTCAGCCTTCGGTATTGCGCTATGGATGGCGTTGCTTTCACCGATGGCCATGGCGACCAGCCTCAA
GGCCCTGGATGTCGCGGCACTGCCGGGGGATCGCCTGGAGTTGAAGCTGACCTTCGATGGACCGGTGCCCGAGCCGCGCG
GCTATACCACCGACCAGCCGGCGCGGATCGCCCTCGATCTGCCCGGTGTCGTCAGCCAGCTGGCAGACAAGAGCCGTGAC
CTGGGCAATGGCAATGCGCGCAGTGTGACGGTGGTCGAATCGGGGCAGCGTACCCGGGTTGTCGTCAATCTGGTCGCGCT
GGCGCCCTACAGCACTCGGGTCGCTGGCAATCAGCTGTTTGTGGTGATCGGCCAGGGGGCGACGGCGGCGCTGAGTGCGC
CGGGGCAGCCGGCTGTCGCGTCGGGGCGGGCCAAGGCCGTGGCTTCCGTGGGGCGGTCGATTCGCAGCGTGGATTTCCAG
CGCGGCGAGCAGGGCGAGGGGAATGTACTGATCGACCTCTCAGACTCGGAAATGAGCCCGGACATACAGGAGCGCGACGG
CAAGCTCGTCCTCACCTTCGCCAAGGCCCGCCTGCCCGAGCCCCTGCGGGTCAGGCTGGACGTCAAGGATTTCGCCACCC
CCGTGCAGTTCGTCAGCGCCCGGGCAGAGTCCGGCAAGGCCATCGTCACCATCGAGCCCGGCGGCACTTTCGATTACTCG
ACCTACCAGACGGATAACAAACTCACGGTGAGCGTCAGGTCGATGACCTCTGGCGACCTGCAGAAACGCAACGCCGAGCG
CTCGGCCTACAACGGCGAAAAGCTCTCGCTGAACTTCCAGGACATCGAGGTTCGCTCGGTGCTGCAACTGATCGCCGACT
TCACCAACCTCAACCTGGTGGCCAGCGATACGGTGCAGGGCGGCATTACCTTGCGCCTGCAGAATGTGCCGTGGGACCAG
GCCCTCGACCTGGTGCTCAAGACCAAGGGGCTGGACAAGCGCAAGGTGGGCAACGTGTTGCTGGTGGCGCCGGCCGATGA
AATCGCTGCCCGCGAACGGCAGGAACTGGAGTCGCAGAAGCAGATCGCCGACCTGGAACCATTGCGGCGCGAACTGCTGC
AAGTCAACTACGCCAAGGCCGCGGAGATCGCCAAGTTGTTCCAGTCGGTCACCCGCGCCGAGGACAAGTCCGACGAACGC
GGGTCGATCACTGTCGATGAGCGGACCAACAACATCATCGCCTACCAGACCCGGGAGCGCCTGGACGAGCTGCGGCGGAT
CGTCAGCCAGCTGGATATCCCGGTGCGCCAGGTGATGATCGAGGCGCGGATCGTCGAGGCCAACGTCGACTACGACAAAA
GCCTGGGCGTGCGCTGGGGCGGCTCGATCCAAAACCAGGGCAACTGGAATGCCTCAGGGGTCAGCAACGGCACCAACGGT
TCCTCCACCATAGGCACGCCGGGCAGCACCAGCAGCAACTCACCGTTCGTCGACCTGGGCACTACTGCCAATACCTCCGG
CCTGGGTATCGCCTTCATCACCGACAATGTGTTGCTGGACCTTGAGCTGACAGCCATGGAAAAGACCGGTAACGGGGAAA
TCGTCTCCCAGCCCAAGGTCGTCACGTCCGACAAGGAGACGGCGAAGATCCTCAAGGGCACGGAAATCCCCTATCAGGAA
GCCGCCTCCAGCGGGGCGACCTCGGTGTCGTTCAAGGAGGCCTCGCTGTCCCTGGAGGTAACGCCACAGATCACCCCGGA
TAACCGCATCATCATGGAGGTCAAGGTCACCAAGGACGAGCCGGACTACCTGAACAAAGTCCAGGACGTGCCGCCGATCA
AGAAGAACGAGGTCAACGCCAAGGTCCTGATCAACGATGGAGAGACCATCGTCATCGGTGGAGTTTTCTCCAATACGCAA
AGCAAGGTTGTAGATAAGGTGCCATTTCTCGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGACGTGGTGTCGGA
GAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAACAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(40-122)

Bacterial type II and III secretion system protein (Secretin)

(525-681)

Bacterial type II/III secretion system short domain (Secretin_N)

(372-437)

Secretin and TonB N terminus short domain (STN)

(298-344)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

70.49

100

0.73
  comQ Acinetobacter baylyi ADP1

39.918

100

0.422
  pilQ Acinetobacter baumannii D1279779

37.903

100

0.409
  pilQ Acinetobacter baumannii strain A118

37.903

100

0.409
  pilQ Legionella pneumophila strain Lp02

37.89

97.536

0.37
  pilQ Legionella pneumophila strain ERS1305867

37.967

96.957

0.368

Multiple sequence alignment