Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   AAG563_RS25575 Genome accession   NZ_CP152341
Coordinates   5667736..5668788 (+) Length   350 a.a.
NCBI ID   WP_037004437.1    Uniprot ID   A0AAX2E8Q7
Organism   Pseudomonas chlororaphis strain NCIMB_15121     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5662736..5673788
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAG563_RS25565 (AAG563_25565) - 5663675..5664943 (+) 1269 WP_007925851.1 malic enzyme-like NAD(P)-binding protein -
  AAG563_RS25570 (AAG563_25570) - 5665084..5667519 (-) 2436 WP_192814744.1 penicillin-binding protein 1A -
  AAG563_RS25575 (AAG563_25575) pilM 5667736..5668788 (+) 1053 WP_037004437.1 pilus assembly protein PilM Machinery gene
  AAG563_RS25580 (AAG563_25580) - 5668788..5669354 (+) 567 WP_009041820.1 PilN domain-containing protein -
  AAG563_RS25585 (AAG563_25585) pilO 5669351..5669968 (+) 618 WP_009041821.1 type 4a pilus biogenesis protein PilO -
  AAG563_RS25590 (AAG563_25590) - 5669965..5670492 (+) 528 WP_009041822.1 pilus assembly protein PilP -
  AAG563_RS25595 (AAG563_25595) pilQ 5670506..5672578 (+) 2073 WP_009041823.1 type IV pilus secretin PilQ Machinery gene
  AAG563_RS25600 (AAG563_25600) aroK 5672583..5673101 (+) 519 WP_009041824.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 37125.48 Da        Isoelectric Point: 4.9975

>NTDB_id=988480 AAG563_RS25575 WP_037004437.1 5667736..5668788(+) (pilM) [Pseudomonas chlororaphis strain NCIMB_15121]
MLGLFSKKASSLLGIDISSTSVKLLELSRVGGRFRVEAYGVEPLPANAVVEKNIVEPEGVGHALSRLLARARPGAKHAAL
AMAGSAVISKVIEMEAGLSDDDRESQLKVDADQYIPYPLDEAAIDFDVLGYSARNPERVDVLLAACRKEHIEVREAALEL
AGLTARVVDLEVCALERSFALAAGSPTARTVALVDIGATLTTLSVVHEGQIVYSREQLFGGHQLTEHIQRRYGLSKEEAG
QAKKQGGLPDDYASEVLQPFQEAVVQQVSRSLQFFQASAPQHQVDSILLAGGSASVPGLVPLIEQRLGLPALIANPFVGM
SIGARVDAAALASEAPSLMIACGLALRSFD

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=988480 AAG563_RS25575 WP_037004437.1 5667736..5668788(+) (pilM) [Pseudomonas chlororaphis strain NCIMB_15121]
GTGCTAGGACTCTTCAGTAAAAAGGCGAGTTCCCTTTTGGGGATCGATATCAGCTCCACCTCGGTCAAGCTTCTTGAATT
GAGCCGTGTCGGTGGCCGCTTTCGGGTCGAGGCCTATGGCGTGGAGCCGTTGCCGGCCAACGCCGTGGTCGAAAAAAACA
TCGTCGAGCCTGAAGGGGTCGGGCATGCCCTGTCTCGCCTGCTGGCCAGGGCCCGGCCGGGTGCGAAGCACGCGGCGCTG
GCGATGGCCGGCTCGGCGGTGATCAGCAAGGTCATCGAGATGGAGGCCGGGCTGTCCGACGACGATCGGGAAAGCCAGCT
CAAGGTCGACGCCGACCAGTACATTCCCTACCCGCTGGACGAGGCCGCCATCGATTTCGACGTCCTCGGCTATTCGGCCC
GCAATCCCGAACGCGTCGATGTGCTGTTGGCGGCCTGCCGCAAGGAGCACATCGAGGTGCGCGAGGCGGCGCTCGAACTT
GCGGGCCTGACTGCGCGAGTGGTGGACCTGGAAGTCTGCGCGCTGGAGCGTTCGTTCGCCCTGGCGGCCGGCTCGCCGAC
TGCCCGGACCGTGGCGCTGGTCGATATCGGCGCCACCCTGACCACCCTGAGCGTCGTGCATGAAGGGCAGATCGTCTATT
CCCGTGAGCAGTTGTTCGGCGGACACCAACTGACCGAACACATCCAGCGCCGCTACGGGCTGTCGAAAGAAGAGGCCGGC
CAGGCAAAAAAGCAGGGTGGCCTGCCGGACGATTACGCCAGTGAGGTGCTGCAACCCTTTCAGGAGGCGGTCGTGCAACA
GGTTTCGCGCTCGTTGCAGTTTTTCCAGGCCTCCGCCCCGCAACACCAGGTCGATTCCATCCTGTTGGCGGGTGGCAGCG
CCTCGGTTCCGGGGCTTGTCCCGTTGATCGAGCAGCGCCTGGGCTTGCCTGCGCTGATCGCCAACCCCTTCGTTGGCATG
AGCATCGGCGCCAGGGTCGACGCCGCAGCCCTGGCTAGCGAGGCCCCGTCCTTGATGATCGCCTGCGGGCTGGCCTTGCG
GAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-349)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.398

100

0.557
  comM Acinetobacter nosocomialis M2

55.114

100

0.554
  comM Acinetobacter baylyi ADP1

52.273

100

0.526
  pilM Legionella pneumophila strain ERS1305867

44.915

100

0.454

Multiple sequence alignment