Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AAG562_RS19700 Genome accession   NZ_CP152340
Coordinates   4254082..4255785 (-) Length   567 a.a.
NCBI ID   WP_003281199.1    Uniprot ID   -
Organism   Stutzerimonas stutzeri strain NCIMB_885     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4249082..4260785
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAG562_RS19670 (AAG562_19670) - 4249219..4250256 (+) 1038 WP_003281190.1 NADP(H)-dependent aldo-keto reductase -
  AAG562_RS19675 (AAG562_19675) - 4250324..4251003 (+) 680 Protein_3868 energy-coupling factor ABC transporter permease -
  AAG562_RS19680 (AAG562_19680) yacG 4251034..4251228 (-) 195 WP_003281194.1 DNA gyrase inhibitor YacG -
  AAG562_RS19685 (AAG562_19685) coaE 4251225..4251833 (-) 609 WP_003281195.1 dephospho-CoA kinase -
  AAG562_RS19690 (AAG562_19690) pilD 4251991..4252860 (-) 870 WP_003281196.1 A24 family peptidase Machinery gene
  AAG562_RS19695 (AAG562_19695) pilC 4252863..4254080 (-) 1218 WP_003281197.1 type II secretion system F family protein Machinery gene
  AAG562_RS19700 (AAG562_19700) pilB 4254082..4255785 (-) 1704 WP_003281199.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AAG562_RS19705 (AAG562_19705) pilA 4256095..4256595 (+) 501 WP_003281200.1 pilin Machinery gene
  AAG562_RS19710 (AAG562_19710) - 4257098..4257652 (+) 555 WP_003281202.1 transposase -
  AAG562_RS19715 (AAG562_19715) cysN 4257895..4259793 (-) 1899 WP_003281204.1 sulfate adenylyltransferase subunit CysN -
  AAG562_RS19720 (AAG562_19720) cysD 4259805..4260722 (-) 918 WP_003281205.1 sulfate adenylyltransferase subunit CysD -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 61958.01 Da        Isoelectric Point: 5.2552

>NTDB_id=988454 AAG562_RS19700 WP_003281199.1 4254082..4255785(-) (pilB) [Stutzerimonas stutzeri strain NCIMB_885]
MSENISLSGLARQMVVAGVLDEKTAQQAQAQAIRSKTPFVTYVVQNKLAKGRAIAELASDQFGIALLDLSALDKESLPKD
LISEKLARQHRVLPLSRRGTKLYVAISDPANHQSIADIQFSTGLSIESILVEDDKLGEAIDKLYESGTTGLEELGEVDLD
GVDVETVNEDGKAEEGGEAADDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKAYRVRLRTDGILHEVARPPIQLAPRISAR
LKVMAGLDISERRKPQDGRIKMKLSKTKAIDFRVNTLPTLWGEKIVMRILDPSSAQMGIDALGYEEDQKELYMNALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTVDVNISTAEDPVEINLEGINQVNVNPKQGMDFSQALRAFLRQDPDIIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVAAFNIATSVNLIIAQRLARKLCNSCKKEVELPREALLEEGFP
ADKIGTFKIYGPIGCENCKGGYKGRVGIYEVVKNTPALARIIMEDGNSIDISTQMRKDGFNDLRASALVKAMQGVTSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=988454 AAG562_RS19700 WP_003281199.1 4254082..4255785(-) (pilB) [Stutzerimonas stutzeri strain NCIMB_885]
ATGAGCGAAAACATTTCCCTATCCGGGCTGGCACGGCAGATGGTCGTGGCCGGCGTGTTGGACGAAAAAACGGCCCAGCA
GGCGCAGGCCCAGGCGATTCGTAGCAAAACACCCTTTGTCACCTATGTAGTGCAGAACAAGTTGGCCAAAGGCCGCGCTA
TAGCTGAGCTTGCATCCGACCAGTTCGGTATCGCCTTGCTGGACTTGTCCGCGCTGGATAAAGAGTCGCTACCCAAGGAT
CTGATAAGCGAGAAGCTGGCGCGTCAGCACCGTGTCTTGCCGCTGTCCAGGCGCGGCACCAAGCTCTATGTGGCCATCTC
CGATCCGGCCAATCACCAGTCGATTGCCGATATCCAGTTCAGTACCGGGCTGAGCATCGAGTCGATCCTGGTGGAAGACG
ACAAGCTGGGCGAGGCGATCGACAAGCTTTACGAAAGCGGCACCACCGGGCTGGAAGAGCTGGGTGAGGTCGATCTGGAT
GGCGTGGATGTCGAGACCGTCAACGAGGATGGAAAAGCCGAGGAGGGCGGAGAGGCCGCTGACGACGCGCCGGTAGTGCG
CTTCGTCAATAAGATGCTGCTCGACGCCATTCGCGGTGGCTCCTCCGACTTGCACTTCGAGCCTTACGAAAAAGCCTACC
GTGTGCGGCTTCGTACGGATGGCATTCTGCATGAAGTCGCTCGCCCACCGATCCAACTGGCGCCCCGCATATCTGCACGC
TTGAAGGTGATGGCCGGGCTGGATATCTCCGAGCGCCGCAAGCCGCAGGACGGGCGGATCAAGATGAAGCTGTCCAAGAC
CAAGGCCATCGACTTTCGGGTGAACACGCTGCCGACGCTGTGGGGCGAGAAGATCGTGATGCGAATCCTTGATCCTTCAA
GTGCTCAAATGGGCATTGATGCGCTGGGTTACGAAGAGGACCAGAAAGAGTTGTACATGAATGCCCTGAAGCAACCTCAG
GGCATGATTTTGGTCACCGGCCCTACGGGATCGGGCAAGACGGTATCCCTTTACACCGGTCTCAATATTCTCAACACGGT
GGATGTGAACATCTCGACCGCCGAAGACCCTGTGGAGATCAACCTTGAGGGTATCAACCAGGTCAACGTCAACCCGAAGC
AGGGTATGGATTTCTCCCAGGCGCTACGCGCGTTTCTGCGTCAGGACCCGGACATCATCATGGTGGGTGAGATTCGCGAC
CTGGAAACAGCCGAAATCGCGATCAAGGCGGCGCAGACGGGCCATATGGTGATGTCGACGCTGCACACCAACAGCGCCGC
TGAAACCCTGACCCGTCTTCGCAATATGGGCGTGGCCGCATTCAATATTGCGACCTCGGTCAACCTGATCATTGCGCAGC
GACTGGCACGCAAACTTTGCAACAGTTGCAAAAAAGAAGTGGAGTTGCCTCGCGAGGCCCTGCTGGAAGAGGGTTTCCCT
GCCGATAAGATCGGAACCTTCAAAATTTACGGCCCGATAGGCTGTGAAAATTGCAAAGGCGGATACAAAGGCCGTGTTGG
TATTTATGAAGTGGTTAAAAACACGCCGGCATTGGCCAGGATTATCATGGAAGACGGCAACTCCATCGACATTTCCACCC
AGATGCGTAAAGACGGCTTCAACGATCTGCGTGCTTCGGCTCTTGTTAAAGCCATGCAGGGCGTGACCAGCCTGGAAGAA
GTGAATCGGGTGACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-147)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.752

99.471

0.594
  pilB Acinetobacter baylyi ADP1

59.397

99.471

0.591
  pilB Legionella pneumophila strain ERS1305867

54.737

100

0.55
  pilB Vibrio campbellii strain DS40M4

49.123

100

0.494
  pilB Vibrio cholerae strain A1552

51.099

96.296

0.492
  pilB Vibrio parahaemolyticus RIMD 2210633

52.308

91.711

0.48
  pilF Neisseria gonorrhoeae MS11

51.154

91.711

0.469

Multiple sequence alignment