Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   AAEU35_RS10470 Genome accession   NZ_CP152294
Coordinates   2061788..2062435 (+) Length   215 a.a.
NCBI ID   WP_058223210.1    Uniprot ID   A0AAP3Z0X8
Organism   Lactococcus lactis strain Q13     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2056788..2067435
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAEU35_RS10445 - 2056912..2058090 (+) 1179 WP_012898345.1 SLC13 family permease -
  AAEU35_RS10450 - 2058263..2058826 (+) 564 WP_004255233.1 GNAT family N-acetyltransferase -
  AAEU35_RS10455 - 2058935..2059333 (+) 399 WP_004255236.1 hypothetical protein -
  AAEU35_RS10460 - 2059438..2060370 (+) 933 WP_004255238.1 ABC transporter ATP-binding protein -
  AAEU35_RS10465 - 2060367..2061728 (+) 1362 WP_021214597.1 ABC transporter permease -
  AAEU35_RS10470 comEA 2061788..2062435 (+) 648 WP_058223210.1 ComEA family DNA-binding protein Machinery gene
  AAEU35_RS10475 comEC 2062416..2064626 (+) 2211 WP_317141310.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  AAEU35_RS10480 - 2064911..2065687 (+) 777 WP_058206315.1 alpha/beta hydrolase family protein -
  AAEU35_RS10485 - 2065872..2066087 (+) 216 WP_004255250.1 F0F1 ATP synthase subunit C -
  AAEU35_RS10490 atpB 2066132..2066845 (+) 714 WP_004255255.1 F0F1 ATP synthase subunit A -
  AAEU35_RS10495 atpF 2066860..2067366 (+) 507 WP_010906128.1 F0F1 ATP synthase subunit B -

Sequence

Protein


Download         Length: 215 a.a.        Molecular weight: 23610.74 Da        Isoelectric Point: 4.8659

>NTDB_id=988202 AAEU35_RS10470 WP_058223210.1 2061788..2062435(+) (comEA) [Lactococcus lactis strain Q13]
MDKILEKVKEYWKMIVLVVCGLIAGGIFYVLTNGQKPTTNLSVENLSSVSRQSSVSKFSEPNEKSVSKIMVDLKGAVTKP
NVYQISSDERLVDLIKEAGGFTDQADQKSINLSAKLKDEEVIYVPKIGENSSTENTDSSANSVTSQVSTTTEKININQAD
VTELQKLTGVGQKKAQDIIDFRTKNGDFKSLEDLGKVSGFGDKTLEKLKDELCFE

Nucleotide


Download         Length: 648 bp        

>NTDB_id=988202 AAEU35_RS10470 WP_058223210.1 2061788..2062435(+) (comEA) [Lactococcus lactis strain Q13]
ATGGATAAGATTTTAGAAAAAGTAAAAGAATATTGGAAAATGATTGTTTTAGTTGTTTGTGGGCTCATTGCTGGTGGGAT
TTTTTACGTTTTAACCAACGGTCAAAAGCCAACTACAAATCTGTCAGTAGAAAATTTAAGTTCTGTCAGCAGGCAAAGCT
CTGTCAGTAAATTTAGTGAACCTAATGAAAAATCTGTCAGTAAAATTATGGTTGATTTAAAAGGTGCGGTAACAAAGCCT
AATGTCTATCAAATTTCGTCAGATGAACGTCTAGTTGATCTAATTAAGGAAGCTGGTGGTTTTACTGACCAAGCTGACCA
GAAATCAATTAATCTGTCAGCAAAATTAAAAGATGAAGAAGTAATTTATGTCCCAAAAATTGGTGAAAATTCAAGCACAG
AAAATACTGATAGCTCTGCTAATTCGGTCACTAGTCAAGTCTCAACAACCACTGAAAAAATAAATATCAATCAAGCAGAT
GTGACTGAACTACAAAAATTAACAGGTGTTGGTCAGAAAAAAGCTCAAGACATCATCGATTTTCGGACGAAAAATGGTGA
TTTCAAATCACTTGAAGACTTGGGAAAAGTTTCTGGTTTTGGAGATAAAACACTAGAAAAATTGAAAGATGAGTTGTGTT
TTGAATAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(151-212)

SLBB domain (SLBB)

(70-125)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Lactococcus lactis subsp. cremoris KW2

75.688

100

0.767
  comEA/celA/cilE Streptococcus mitis SK321

44.444

100

0.447
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

43.119

100

0.437
  comEA/celA/cilE Streptococcus pneumoniae Rx1

41.096

100

0.419
  comEA/celA/cilE Streptococcus pneumoniae D39

41.096

100

0.419
  comEA/celA/cilE Streptococcus pneumoniae R6

41.096

100

0.419
  comEA/celA/cilE Streptococcus mitis NCTC 12261

41.284

100

0.419
  comEA Latilactobacillus sakei subsp. sakei 23K

36.564

100

0.386
  comEA Staphylococcus aureus MW2

37.559

99.07

0.372

Multiple sequence alignment