Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   KNP07_RS24310 Genome accession   NZ_CP152075
Coordinates   5221303..5223003 (+) Length   566 a.a.
NCBI ID   WP_128672463.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain NCTR 501     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5216303..5228003
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KNP07_RS24290 (KNP07_24295) nadC 5218128..5218976 (+) 849 WP_003110344.1 carboxylating nicotinate-nucleotide diphosphorylase -
  KNP07_RS24300 (KNP07_24305) - 5219157..5220542 (-) 1386 WP_134279314.1 O-antigen ligase family protein -
  KNP07_RS24305 (KNP07_24310) - 5220608..5221072 (-) 465 WP_124089607.1 pilin -
  KNP07_RS24310 (KNP07_24315) pilB 5221303..5223003 (+) 1701 WP_128672463.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KNP07_RS24315 (KNP07_24320) pilC 5223007..5224224 (+) 1218 WP_003107299.1 type II secretion system F family protein Machinery gene
  KNP07_RS24320 (KNP07_24325) pilD 5224225..5225097 (+) 873 WP_134279316.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  KNP07_RS24325 (KNP07_24330) coaE 5225094..5225705 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  KNP07_RS24330 (KNP07_24335) yacG 5225702..5225902 (+) 201 WP_017148551.1 DNA gyrase inhibitor YacG -
  KNP07_RS24335 (KNP07_24340) - 5225939..5226148 (-) 210 WP_003094660.1 hypothetical protein -
  KNP07_RS24340 (KNP07_24345) - 5226254..5226943 (-) 690 WP_003103868.1 energy-coupling factor ABC transporter permease -
  KNP07_RS24345 (KNP07_24350) - 5226940..5227410 (-) 471 WP_003094664.1 hypothetical protein -
  KNP07_RS24350 (KNP07_24355) - 5227407..5227832 (-) 426 WP_003103865.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62356.26 Da        Isoelectric Point: 5.4489

>NTDB_id=987206 KNP07_RS24310 WP_128672463.1 5221303..5223003(+) (pilB) [Pseudomonas aeruginosa strain NCTR 501]
MNDSTPLSGLAKQLVLNELLDEKTARQAQVQAARNKQSLVSHLVQAKLVQSRALVEVAAEQFGIAYCDLNSLDRESIPKD
VISERLVRQHRVLPLWKRGNKLFIGLSDPTNHQAVTDIQFSTGLTTEALLVEDDKLGVAIEKLYEGAMDALGDLGDIDLD
GVDVGKAENGSQEDSGDDAEDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKTYRVRFRTDGMLHEVAKPPIQLASRISARL
KVMAGLDISERRKPQDGRIKMRVSKTKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHDVPKETLLHEGFPE
ELIGTFKLYSPVGCENCKGGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=987206 KNP07_RS24310 WP_128672463.1 5221303..5223003(+) (pilB) [Pseudomonas aeruginosa strain NCTR 501]
ATGAACGATTCCACGCCTCTCAGCGGCCTTGCCAAGCAACTGGTCCTGAACGAACTCCTCGACGAAAAGACCGCCCGCCA
GGCCCAGGTGCAGGCCGCGCGCAACAAGCAGTCGCTGGTCAGCCACCTGGTCCAGGCCAAGCTGGTACAGAGCCGGGCGT
TGGTCGAGGTAGCCGCCGAGCAGTTCGGCATCGCCTATTGCGACCTGAACAGCCTGGATCGGGAAAGCATTCCGAAGGAT
GTCATCAGCGAGCGCCTGGTCCGCCAGCACCGCGTGTTGCCGCTGTGGAAACGCGGCAACAAACTGTTCATCGGCCTCTC
CGACCCAACCAATCACCAAGCGGTCACCGATATCCAGTTCAGCACCGGCCTCACCACCGAGGCCCTGCTGGTGGAGGACG
ACAAGCTCGGCGTAGCCATCGAGAAGCTCTACGAGGGCGCCATGGATGCCCTCGGCGATCTCGGCGACATCGATCTCGAT
GGAGTCGATGTGGGCAAGGCAGAAAACGGCAGCCAGGAAGATTCCGGCGACGACGCCGAAGATGCCCCGGTGGTGCGCTT
CGTCAACAAGATGCTGCTCGACGCCATCCGCGGCGGCTCTTCCGACCTGCACTTCGAGCCCTACGAAAAGACCTACCGGG
TGCGCTTCCGTACCGACGGCATGCTCCACGAAGTGGCCAAGCCGCCGATCCAGTTGGCCAGCCGCATTTCCGCGCGGCTC
AAGGTGATGGCCGGCCTGGATATCTCCGAACGACGCAAACCGCAGGACGGACGGATCAAGATGCGCGTGTCGAAGACCAA
GTCCATCGACTTCCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTTATGCGGATCCTCGACTCCTCCAGCG
CGCAGATGGGCATCGACGCCCTCGGCTACGAAGAGGATCAGAAGGAACTTTATCTGGCCGCGCTCAAACAGCCACAGGGC
ATGATCCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTTTCGCTGTACACCGGCCTGAATATCCTCAATACCACCGA
CATCAATATCTCCACCGCCGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAACCCGCGCCAGG
GCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGACCTG
GAAACCGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGCCGA
GACCCTGACCCGGCTGCTGAACATGGGCGTGCCGGCGTTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGCGCC
TTGCGCGAAAACTCTGTTCGCACTGCAAGAAAGAACACGACGTGCCGAAGGAAACCCTGCTTCACGAAGGTTTCCCCGAG
GAGCTGATCGGCACCTTCAAGCTGTATTCGCCTGTAGGCTGCGAAAACTGCAAAGGCGGTTACAAAGGTCGCGTCGGTAT
TTATGAAGTGGTTAAAAACACCCCCGCCCTGCAGCGGATTATCATGGAAGAAGGCAACTCCATCGAGATCGCCGAGCAAG
CCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGAATCACCAGCCTGGAGGAAGTC
AACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.719

100

0.581
  pilB Acinetobacter baylyi ADP1

56.614

100

0.567
  pilB Legionella pneumophila strain ERS1305867

54.321

100

0.544
  pilB Vibrio cholerae strain A1552

51.699

98.763

0.511
  pilF Neisseria gonorrhoeae MS11

50.355

99.647

0.502
  pilB Vibrio parahaemolyticus RIMD 2210633

52.345

94.17

0.493
  pilB Vibrio campbellii strain DS40M4

51.407

94.17

0.484
  pilB/pilB1 Synechocystis sp. PCC 6803

37.763

100

0.412
  pilF Thermus thermophilus HB27

38.84

100

0.39
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.909

97.173

0.378

Multiple sequence alignment