Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AAFX31_RS12780 Genome accession   NZ_CP151941
Coordinates   1048940..1050625 (+) Length   561 a.a.
NCBI ID   WP_411062150.1    Uniprot ID   -
Organism   Vibrio chagasii strain TBV018     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1043940..1055625
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAFX31_RS12760 (AAFX31_12770) pdhR 1045437..1046198 (-) 762 WP_008222726.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  AAFX31_RS12765 (AAFX31_12775) ampD 1046682..1047282 (-) 601 Protein_861 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  AAFX31_RS12770 (AAFX31_12780) nadC 1047366..1048256 (+) 891 WP_004738163.1 carboxylating nicotinate-nucleotide diphosphorylase -
  AAFX31_RS12775 (AAFX31_12785) - 1048511..1048939 (+) 429 WP_411062149.1 pilin -
  AAFX31_RS12780 (AAFX31_12790) pilB 1048940..1050625 (+) 1686 WP_411062150.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AAFX31_RS12785 (AAFX31_12795) pilC 1050641..1051870 (+) 1230 WP_411062151.1 type II secretion system F family protein Machinery gene
  AAFX31_RS12790 (AAFX31_12800) pilD 1051937..1052806 (+) 870 WP_102426438.1 prepilin peptidase Machinery gene
  AAFX31_RS12795 (AAFX31_12805) coaE 1052809..1053423 (+) 615 WP_061032470.1 dephospho-CoA kinase -
  AAFX31_RS12800 (AAFX31_12810) zapD 1053450..1054190 (+) 741 WP_061032469.1 cell division protein ZapD -
  AAFX31_RS12805 (AAFX31_12815) yacG 1054271..1054468 (+) 198 WP_008222354.1 DNA gyrase inhibitor YacG -
  AAFX31_RS12810 (AAFX31_12820) rplS 1054841..1055194 (-) 354 WP_004738151.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62193.98 Da        Isoelectric Point: 4.9267

>NTDB_id=981398 AAFX31_RS12780 WP_411062150.1 1048940..1050625(+) (pilB) [Vibrio chagasii strain TBV018]
MLTNLPTVLRQADLLSLTQEQAVAEHVQASGISTPEALLVLDLFTGDSLAHNIQTIFGLPLVQLNNTDYEALCDQLGLRE
LITKYRAIPISVSNSTLTLASADPTDLQAEDDFRFATGLQIELAVANYSELEGAIRKLYGRSISGQDSKRKEITQDELAN
LVEVSDDELASIEDLSQDDSPVSRFINQILVDAVRKGASDIHFEPYEEHYRVRLRCDGILVEIQQPASHLSRRLSARLKI
LAKLDIAERRLPQDGRIKLRLNDELAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYSDDQKALYLNALKRPQGMI
LMTGPTGSGKTVSLYTGLRVLNTIERNISTAEDPVEINLCGINQVQVMPKIGFGFAEALRSFLRQDPDVVMVGEIRDLET
AEIAIKASQTGHLVLSTLHTNSAAETVTRLAHMGIEPFNLASSLSLIIAQRLARRLCNQCKVIDDSPDIFLRHSIPSSKT
IYKANPQGCNECNQGYSGRVGIYEVMPFTDKLKNSLISKPNALEVEDLARREGMRTLQESGLDKLLEGTTSYQELQRVLY
L

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=981398 AAFX31_RS12780 WP_411062150.1 1048940..1050625(+) (pilB) [Vibrio chagasii strain TBV018]
GTGCTCACCAACCTCCCTACAGTTCTCCGTCAGGCTGACTTACTTAGCCTGACTCAAGAACAAGCCGTGGCGGAACATGT
ACAAGCTTCAGGTATTTCAACACCTGAAGCTTTACTCGTTCTTGATCTCTTCACAGGCGACTCCCTAGCACACAATATAC
AAACCATCTTCGGCTTACCCTTAGTACAACTTAACAACACAGATTATGAAGCTTTGTGTGATCAACTAGGGCTTCGCGAG
TTAATCACCAAATATCGTGCTATTCCGATTTCAGTCTCTAACTCGACGCTTACACTTGCTTCCGCCGACCCAACCGACTT
ACAAGCTGAAGATGATTTTCGCTTTGCAACCGGGTTGCAGATAGAACTGGCAGTCGCCAATTACTCTGAACTCGAAGGTG
CAATACGCAAGTTGTACGGCCGCTCGATTTCCGGGCAAGATTCTAAGCGTAAAGAGATCACCCAAGATGAACTGGCTAAC
CTAGTTGAAGTGTCTGACGACGAGCTCGCATCGATTGAAGACCTTAGTCAAGACGACTCTCCAGTTAGCCGATTTATCAA
TCAGATACTGGTCGATGCGGTACGTAAAGGCGCATCAGATATCCATTTTGAACCTTACGAAGAGCACTACCGTGTGCGCC
TACGTTGTGACGGTATATTGGTTGAAATCCAACAACCCGCTTCTCACTTAAGCCGTCGTTTATCCGCCCGTCTGAAAATT
CTCGCCAAGCTTGATATTGCTGAGCGTCGCTTACCTCAAGATGGACGAATAAAACTGCGCTTAAATGACGAACTCGCGAT
TGATATGCGCGTCTCCACATTGCCCACCTTGTGGGGCGAGAAAATTGTACTAAGACTACTCGATAGCAGCGCTGCCAATC
TCGATATCGACAAGCTCGGTTACAGCGATGATCAAAAAGCCCTCTACCTGAATGCACTAAAACGCCCGCAAGGGATGATC
TTAATGACTGGCCCTACTGGCAGCGGAAAAACCGTCTCCCTTTATACCGGCCTTCGAGTATTGAACACCATAGAACGTAA
TATCTCTACTGCAGAAGATCCGGTAGAGATTAACCTATGTGGTATAAATCAAGTGCAAGTAATGCCAAAGATTGGCTTTG
GGTTCGCTGAAGCCCTGCGCTCGTTTTTACGACAAGATCCAGATGTGGTGATGGTTGGTGAGATCCGTGATTTAGAAACT
GCAGAAATCGCCATCAAAGCATCGCAAACCGGTCACTTAGTGCTTTCGACTCTCCATACTAACTCTGCGGCTGAAACCGT
TACCCGACTCGCTCATATGGGGATTGAGCCCTTTAACTTAGCCTCATCATTGAGCTTGATCATTGCCCAGCGACTGGCAA
GACGCTTGTGCAACCAATGCAAAGTCATTGACGACTCACCAGACATCTTCCTGCGTCACTCAATACCTAGCAGCAAAACC
ATTTATAAGGCTAACCCACAAGGGTGTAACGAGTGCAATCAAGGCTATTCTGGGCGTGTCGGAATTTATGAAGTGATGCC
ATTCACCGACAAACTCAAAAATAGCCTTATCAGCAAACCGAATGCGTTGGAGGTTGAAGACCTCGCTCGCCGAGAAGGGA
TGCGCACACTGCAAGAGTCGGGATTAGATAAGCTACTTGAAGGCACCACCAGCTATCAAGAGCTGCAACGTGTTCTGTAC
CTATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-141)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

73.31

100

0.734
  pilB Vibrio campbellii strain DS40M4

70.463

100

0.706
  pilB Vibrio parahaemolyticus RIMD 2210633

70.285

100

0.704
  pilB Legionella pneumophila strain ERS1305867

48.188

98.396

0.474
  pilB Acinetobacter baumannii D1279779

50.581

91.979

0.465
  pilB Acinetobacter baylyi ADP1

49.419

91.979

0.455
  pilF Neisseria gonorrhoeae MS11

43.243

98.93

0.428
  pilF Thermus thermophilus HB27

38.772

92.87

0.36

Multiple sequence alignment