Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AAGT95_RS18625 Genome accession   NZ_CP151919
Coordinates   4120792..4122519 (+) Length   575 a.a.
NCBI ID   WP_110602867.1    Uniprot ID   -
Organism   Salinicola lusitanus strain LLJ914     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4115792..4127519
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAGT95_RS18600 (AAGT95_18600) - 4116864..4117619 (+) 756 WP_342594752.1 RIO1 family regulatory kinase/ATPase -
  AAGT95_RS18605 (AAGT95_18605) - 4117616..4117768 (+) 153 WP_342594753.1 hypothetical protein -
  AAGT95_RS18610 (AAGT95_18610) - 4117784..4119235 (-) 1452 WP_342594754.1 TrkH family potassium uptake protein -
  AAGT95_RS18615 (AAGT95_18615) - 4119588..4119872 (+) 285 WP_110602865.1 hypothetical protein -
  AAGT95_RS18620 (AAGT95_18620) - 4119934..4120422 (-) 489 WP_342594755.1 pilin -
  AAGT95_RS18625 (AAGT95_18625) pilB 4120792..4122519 (+) 1728 WP_110602867.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AAGT95_RS18630 (AAGT95_18630) pilC 4122561..4123814 (+) 1254 WP_342594756.1 type II secretion system F family protein Machinery gene
  AAGT95_RS18635 (AAGT95_18635) - 4123951..4124811 (+) 861 WP_342594757.1 A24 family peptidase -
  AAGT95_RS18640 (AAGT95_18640) coaE 4124850..4125458 (+) 609 WP_342596548.1 dephospho-CoA kinase -
  AAGT95_RS18645 (AAGT95_18645) yacG 4125513..4125758 (+) 246 WP_110602871.1 DNA gyrase inhibitor YacG -
  AAGT95_RS18650 (AAGT95_18650) - 4125970..4126923 (-) 954 WP_110602384.1 Nudix family hydrolase -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 63205.46 Da        Isoelectric Point: 5.5909

>NTDB_id=981178 AAGT95_RS18625 WP_110602867.1 4120792..4122519(+) (pilB) [Salinicola lusitanus strain LLJ914]
MPDAIPSRAQSGLAQRFVEQGLISAEACEQAFREAEKRQLTLLEFLVREGRIDARQASVLAAREYGLPFLDLKQIDWDQL
PVIEPVADKVIRRFQVLPLARDHRRLTVAIAFPATLASLDELQFTTGLAIEGVLVPFDQLERALAKRYQNHETSGMLEAL
GEASEALTLDTSEADLENEAAAFDKGGDDDEPVVRFVNKILRDAIHRGASDIHFEPYESSYRIRFRVDGMLVEIARPPLN
LRTRLAARLKVMARLDISERRLPQDGAMKIRLDAKRAMDFRVNTLPTVFGEKIVLRLLDAETASFGIDALGFAPDQRRLF
EAALAEPQGMILVTGPTGSGKTVTLYTGINLLNTEERNISTAEDPVELKVPGVNQVNVQPRIGLDFASALRAFLRQDPDV
VMVGEIRDLETAEIAVKAAQTGHLVLSTLHTNSAAETLTRLRNMGVASFNIASSVSLIIAQRLARLLCPRCKTPAEIPAE
VLHREGVSAERLAGATLYRAVGCDHCTLGYKGRIGIYEVVPVSEAMSRMIMADSHALELARAARDAGHAGLRQSGLDKVL
QGLTSLEEINRVTTD

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=981178 AAGT95_RS18625 WP_110602867.1 4120792..4122519(+) (pilB) [Salinicola lusitanus strain LLJ914]
ATGCCGGACGCCATACCCTCCCGCGCGCAGAGCGGGCTCGCCCAGCGCTTCGTCGAGCAGGGATTGATCAGCGCCGAGGC
TTGCGAGCAGGCGTTCCGCGAGGCCGAAAAGAGGCAATTGACGCTACTGGAGTTCCTGGTGAGAGAGGGCCGGATCGATG
CCCGCCAGGCCAGCGTGCTGGCGGCTCGCGAGTACGGCTTGCCATTCCTGGATTTGAAGCAGATCGACTGGGATCAGCTC
CCGGTCATCGAACCGGTGGCGGACAAGGTCATTCGCCGCTTCCAGGTGCTGCCGCTGGCCCGCGACCACCGGCGACTGAC
CGTAGCGATCGCGTTTCCCGCCACCTTGGCCAGTCTGGACGAGCTGCAGTTCACCACCGGGCTGGCGATCGAGGGCGTGC
TGGTGCCATTCGATCAGTTGGAACGGGCGCTGGCCAAGCGCTATCAGAACCACGAGACCTCCGGCATGCTGGAGGCGCTC
GGGGAGGCCAGCGAGGCCCTGACGCTGGATACCAGCGAAGCGGATCTGGAAAACGAGGCCGCCGCCTTCGACAAGGGCGG
CGACGACGATGAGCCGGTGGTTCGTTTCGTCAACAAGATCCTGCGGGACGCCATCCATCGCGGCGCGTCCGACATTCACT
TCGAGCCCTACGAGTCCAGCTATCGGATCCGCTTTCGGGTCGATGGCATGCTGGTCGAGATCGCGCGCCCCCCGCTCAAC
CTGCGCACCCGCCTGGCTGCGCGACTCAAGGTGATGGCACGCCTGGATATCTCCGAACGCCGCCTGCCCCAGGACGGCGC
GATGAAGATCCGGCTCGATGCCAAGCGTGCCATGGACTTCCGCGTCAATACGCTGCCCACCGTTTTCGGCGAGAAGATCG
TGCTGCGTCTGCTGGATGCCGAGACCGCCAGCTTCGGCATCGACGCACTGGGCTTCGCTCCCGATCAGCGTCGGCTGTTC
GAGGCCGCGCTGGCCGAGCCCCAGGGCATGATCCTGGTCACCGGCCCCACCGGTAGCGGCAAGACGGTGACGCTCTACAC
CGGTATCAATCTGCTCAATACCGAGGAGCGCAATATCTCGACCGCGGAAGACCCGGTGGAGCTCAAGGTTCCCGGCGTCA
ATCAGGTCAACGTGCAGCCGCGTATCGGTCTCGACTTCGCCAGCGCCCTTCGCGCTTTCCTGCGCCAGGACCCGGACGTG
GTGATGGTCGGCGAGATTCGCGACCTGGAGACCGCCGAGATCGCGGTCAAGGCGGCCCAGACCGGCCACCTGGTGCTCTC
GACCCTGCACACCAACTCGGCCGCCGAAACGCTGACCCGCCTGCGCAACATGGGCGTCGCCAGCTTCAATATCGCCAGTT
CGGTATCGCTGATCATTGCCCAGCGCCTGGCGCGACTGCTGTGCCCGCGCTGCAAGACGCCGGCGGAGATTCCTGCCGAA
GTGCTGCACCGGGAAGGCGTCTCAGCCGAGCGACTGGCAGGCGCCACCCTCTACCGCGCGGTGGGGTGCGATCACTGCAC
GCTGGGCTACAAGGGTCGAATCGGTATCTACGAGGTCGTGCCGGTCAGCGAGGCGATGAGCCGGATGATCATGGCGGACA
GCCATGCCCTCGAACTGGCCCGCGCAGCTCGGGATGCCGGTCATGCGGGGCTGCGCCAGAGCGGGCTCGACAAGGTTCTC
CAGGGCCTGACCAGCCTCGAGGAGATCAACCGCGTAACGACGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(199-466)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

51.916

99.826

0.518
  pilB Acinetobacter baumannii D1279779

51.146

98.609

0.504
  pilB Legionella pneumophila strain ERS1305867

51.15

98.261

0.503
  pilB Vibrio parahaemolyticus RIMD 2210633

48.415

98.783

0.478
  pilB Vibrio campbellii strain DS40M4

48.829

96.522

0.471
  pilF Neisseria gonorrhoeae MS11

46.76

99.304

0.464
  pilB Vibrio cholerae strain A1552

51.262

89.565

0.459
  pilB/pilB1 Synechocystis sp. PCC 6803

36.953

100

0.397
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.366

97.913

0.376
  pilF Thermus thermophilus HB27

36.907

98.957

0.365

Multiple sequence alignment