Detailed information NTG0097789

Bioinformatically predicted

Overview

• Name: comEA/celA/cilE
• Type: Machinery gene
• Locus tag: R8619_RS04425
• Genome accession: NZ_AP026918
• Coordinates: 862964..863614 (+)
• Length: 216 a.a.
• NCBI ID: WP_000387336.1
• Uniprot ID: -
• Organism: Streptococcus pneumoniae strain PZ900700009
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 857964..868614

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
R8619_RS04395 (PC0009_08620)	pyrH	858688..859425 (+)	738	WP_000002997.1	UMP kinase	-
R8619_RS04400 (PC0009_08630)	frr	859434..859991 (+)	558	WP_000024409.1	ribosome recycling factor	-
R8619_RS04405 (PC0009_08640)	cvfB	860051..860905 (+)	855	WP_001095445.1	RNA-binding virulence regulatory protein CvfB	-
R8619_RS04410 (PC0009_08650)	-	860914..861129 (+)	216	WP_001232085.1	YozE family protein	-
R8619_RS04415 (PC0009_08660)	-	861215..862219 (+)	1005	WP_000658185.1	PhoH family protein	-
R8619_RS04420 (PC0009_08670)	-	862327..862896 (+)	570	WP_000443751.1	GNAT family N-acetyltransferase	-
R8619_RS04425 (PC0009_08680)	comEA/celA/cilE	862964..863614 (+)	651	WP_000387336.1	ComEA family DNA-binding protein	Machinery gene
R8619_RS04430 (PC0009_08690)	comEC/celB	863598..865838 (+)	2241	WP_000942403.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
R8619_RS04435 (PC0009_08700)	-	866017..866205 (+)	189	WP_001809102.1	hypothetical protein	-
R8619_RS04440 (PC0009_08710)	-	866237..866824 (+)	588	WP_000933542.1	ATP-binding cassette domain-containing protein	-
R8619_RS04445 (PC0009_08720)	-	866828..868009 (+)	1182	WP_000655950.1	membrane protein	-

Sequence

Protein

• Length: 216 a.a.
• Molecular weight: 23189.54 Da
• Isoelectric Point: 5.5171

>NTDB_id=97789 R8619_RS04425 WP_000387336.1 862964..863614(+) (comEA/celA/cilE) [Streptococcus pneumoniae strain PZ900700009]
MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTPVKETNLQAEVAAVSKDLVSEKEVNKEEKEEPVEQDLITVDVKG
AVKSPGIYDLPVGSRVNDAVQKAGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGSGTASSISKEKKVNLNKA
SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGGKTIEKLKDYVTVD

Nucleotide

• Length: 651 bp

>NTDB_id=97789 R8619_RS04425 WP_000387336.1 862964..863614(+) (comEA/celA/cilE) [Streptococcus pneumoniae strain PZ900700009]
ATGGAAGCAATTATCGAGAAAATCAAAGAGTATAAAATCATCGTCATCTGTACTGGTCTGGGCTTGCTTGTAGGCGGATT
TTTCCTGCTAAAGCCAGCTCCACAAACACCTGTAAAGGAAACGAATTTGCAGGCTGAAGTTGCAGCTGTTTCCAAGGATT
TGGTATCCGAAAAGGAAGTGAACAAGGAAGAGAAGGAAGAACCAGTTGAACAAGATCTAATCACAGTAGATGTCAAAGGT
GCTGTCAAATCGCCAGGGATTTATGACTTGCCTGTAGGTAGTCGAGTCAATGATGCTGTTCAGAAGGCTGGTGGCTTGAC
AGAGCAAGCAGACAGCAAGTCGCTCAATCTAGCTCAGAAAGTTAGTGATGAGGCTCTGGTTTACGTTCCTACTAAGGGAG
AAGAAGCAGTTAGTCAACAGACTGGTTCGGGGACAGCTTCTTCAATAAGCAAGGAAAAGAAGGTCAATCTCAACAAGGCC
AGTCTGGAAGAACTCAAGCAGGTCAAGGGACTGGGAGGAAAACGAGCTCAGGACATTATCGACCATCGTGAGGCAAATGG
CAAGTTCAAGTCAGTAGACGAGCTCAAGAAGGTCTCTGGCATTGGTGGCAAAACAATAGAAAAGCTTAAAGACTATGTTA
CAGTGGATTAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (76-126)

• Helix-hairpin-helix motif (HHH_3) (151-214)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA/celA/cilE	Streptococcus pneumoniae Rx1	99.074	100	0.991
comEA/celA/cilE	Streptococcus pneumoniae D39	99.074	100	0.991
comEA/celA/cilE	Streptococcus pneumoniae R6	99.074	100	0.991
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	97.222	100	0.972
comEA/celA/cilE	Streptococcus mitis NCTC 12261	95.833	100	0.958
comEA/celA/cilE	Streptococcus mitis SK321	91.204	100	0.912
comEA	Lactococcus lactis subsp. cremoris KW2	43.612	100	0.458
comEA	Bacillus subtilis subsp. subtilis str. 168	41.579	87.963	0.366
comEA	Latilactobacillus sakei subsp. sakei 23K	33.476	100	0.361