Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA/celA/cilE   Type   Machinery gene
Locus tag   R8522_RS04540 Genome accession   NZ_AP026916
Coordinates   889050..889700 (+) Length   216 a.a.
NCBI ID   WP_050287616.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain PZ900700006     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 884050..894700
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R8522_RS04510 (PC0006_09150) - 884534..884749 (+) 216 WP_001232082.1 YozE family protein -
  R8522_RS04515 (PC0006_09160) - 884835..885803 (+) 969 WP_000658183.1 PhoH family protein -
  R8522_RS04520 (PC0006_09170) - 885996..886496 (+) 501 WP_000566988.1 GNAT family N-acetyltransferase -
  R8522_RS04525 (PC0006_09180) - 886499..886825 (+) 327 Protein_899 TfoX/Sxy family protein -
  R8522_RS04530 ald 887126..888237 (-) 1112 Protein_900 alanine dehydrogenase -
  R8522_RS04535 (PC0006_09220) - 888414..888983 (+) 570 WP_000443743.1 GNAT family N-acetyltransferase -
  R8522_RS04540 (PC0006_09230) comEA/celA/cilE 889050..889700 (+) 651 WP_050287616.1 helix-hairpin-helix domain-containing protein Machinery gene
  R8522_RS04545 - 889684..891925 (+) 2242 Protein_903 DNA internalization-related competence protein ComEC/Rec2 -
  R8522_RS04550 (PC0006_09260) - 892104..892292 (+) 189 WP_001809102.1 hypothetical protein -
  R8522_RS04555 (PC0006_09270) - 892324..892911 (+) 588 WP_000933542.1 ATP-binding cassette domain-containing protein -
  R8522_RS04560 - 892915..893703 (+) 789 Protein_906 hypothetical protein -

Sequence

Protein


Download         Length: 216 a.a.        Molecular weight: 23243.68 Da        Isoelectric Point: 5.5171

>NTDB_id=97636 R8522_RS04540 WP_050287616.1 889050..889700(+) (comEA/celA/cilE) [Streptococcus pneumoniae strain PZ900700006]
MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTPVKETNLQAEVAAVSKDLVSEKEVNKEEKEEPLEQDLITVDVKG
AVKSPGIYDLPVGSRVNDAVQKAGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGLGTASSISKEKKVNLNKA
SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGAKTIEKLKDYVTVD

Nucleotide


Download         Length: 651 bp        

>NTDB_id=97636 R8522_RS04540 WP_050287616.1 889050..889700(+) (comEA/celA/cilE) [Streptococcus pneumoniae strain PZ900700006]
ATGGAAGCAATTATCGAGAAAATCAAAGAGTATAAAATCATCGTCATCTGTACTGGTCTGGGCTTACTTGTAGGAGGATT
TTTCCTGCTAAAACCAGCTCCACAAACACCTGTCAAAGAGACGAATTTGCAGGCTGAAGTCGCAGCTGTTTCCAAGGATT
TGGTATCCGAAAAGGAAGTGAACAAGGAAGAAAAGGAAGAACCCCTTGAACAAGATCTAATCACAGTAGATGTCAAAGGT
GCTGTCAAATCGCCAGGGATTTATGACTTGCCTGTAGGTAGTCGAGTCAATGATGCTGTTCAGAAGGCTGGTGGCTTGAC
AGAGCAAGCAGACAGCAAGTCGCTCAATCTAGCTCAGAAAGTTAGTGATGAGGCTCTGGTTTACGTTCCTACTAAGGGAG
AAGAAGCAGTTAGCCAACAGACTGGTTTGGGGACAGCTTCTTCAATAAGCAAGGAAAAGAAGGTCAATCTCAACAAGGCT
AGTCTGGAAGAACTCAAGCAGGTCAAGGGACTGGGAGGAAAACGAGCTCAGGACATTATCGACCATCGTGAGGCAAATGG
CAAGTTCAAATCAGTAGACGAGCTCAAGAAGGTCTCTGGCATTGGTGCCAAGACTATAGAAAAGTTAAAAGACTATGTTA
CAGTGGATTAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(151-214)

SLBB domain (SLBB)

(76-126)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA/celA/cilE Streptococcus pneumoniae Rx1

99.537

100

0.995
  comEA/celA/cilE Streptococcus pneumoniae D39

99.537

100

0.995
  comEA/celA/cilE Streptococcus pneumoniae R6

99.537

100

0.995
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

96.759

100

0.968
  comEA/celA/cilE Streptococcus mitis NCTC 12261

96.759

100

0.968
  comEA/celA/cilE Streptococcus mitis SK321

90.741

100

0.907
  comEA Lactococcus lactis subsp. cremoris KW2

43.172

100

0.454
  comEA Bacillus subtilis subsp. subtilis str. 168

41.579

87.963

0.366
  comEA Latilactobacillus sakei subsp. sakei 23K

32.773

100

0.361

Multiple sequence alignment