Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AADH33_RS12610 Genome accession   NZ_CP151283
Coordinates   3040526..3042241 (+) Length   571 a.a.
NCBI ID   WP_348549108.1    Uniprot ID   A0AAU6RZW3
Organism   Psychrobacter sp. KFRI-CH2-11     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3035526..3047241
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AADH33_RS12580 (AADH33_12570) nusA 3035581..3037065 (-) 1485 WP_100748657.1 transcription termination factor NusA -
  AADH33_RS12585 (AADH33_12575) rimP 3037179..3037679 (-) 501 WP_075101367.1 ribosome maturation factor RimP -
  AADH33_RS12600 (AADH33_12590) secG 3038626..3038934 (-) 309 WP_075101365.1 preprotein translocase subunit SecG -
  AADH33_RS12605 (AADH33_12595) tpiA 3039366..3040175 (-) 810 WP_348549107.1 triose-phosphate isomerase -
  AADH33_RS12610 (AADH33_12600) pilB 3040526..3042241 (+) 1716 WP_348549108.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AADH33_RS12615 (AADH33_12605) pilC 3042612..3043835 (+) 1224 WP_075101362.1 type II secretion system F family protein Machinery gene
  AADH33_RS12620 (AADH33_12610) pilD 3043861..3044763 (+) 903 WP_348549109.1 prepilin peptidase Machinery gene
  AADH33_RS12625 (AADH33_12615) coaE 3044939..3045625 (+) 687 WP_348549110.1 dephospho-CoA kinase -
  AADH33_RS12630 (AADH33_12620) rlmB 3045588..3046358 (-) 771 WP_100748650.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  AADH33_RS12635 (AADH33_12625) - 3046626..3046961 (-) 336 WP_075101358.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62823.15 Da        Isoelectric Point: 4.9376

>NTDB_id=976083 AADH33_RS12610 WP_348549108.1 3040526..3042241(+) (pilB) [Psychrobacter sp. KFRI-CH2-11]
MSITTSKYGGLAQQLISEGIVSEANMKTAQTESQQQQIGLVSYLVDNKLADAYHLAQMLSQAFGDPLFDLDALSLDVIPK
DLVDEKIVRKFNALPLFKRGQRLFVALSDPTRIDAIDAIAFNSRLSIETVVVEEDKLKKRIEALYADSMQGLDSFSDSDL
NVGFEDGDDAADEGETKLSDSVDEAPVVKFVNKMLVDAIRMGASDLHFEPYEKTYRVRFRVDGVMQKMASPPVQLAGKIA
ARLKVMSQMDISERRIPQDGRIKLKLSKNKAIDFRVNSLPTLFGEKIVLRILDPSSAMLGIDALGYEPDQQEMFLEALHK
PQGMLLITGPTGSGKTVSLYTGINILNTGATNISTAEDPVEINLEGINQVNVNPKVGLTFANALKSFLRQDPDIVMVGEI
RDLETAEIAIKAAQTGHMVLSTLHTNSAPETLTRLRNMGVASFNIATSVNLVIAQRLARRLCKNCKKAINVPRQSLLELG
FTDADLDDPDNVIYEPVGCSECREGYKGRVGVYEVMKVSPDISRIIMEDGNAMDIKDAALKNGFRDLRRSGILKVLQGVT
SIQEMMRVTSE

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=976083 AADH33_RS12610 WP_348549108.1 3040526..3042241(+) (pilB) [Psychrobacter sp. KFRI-CH2-11]
ATGTCTATCACAACCAGCAAATATGGTGGTTTAGCGCAGCAGCTGATTAGCGAAGGTATCGTTAGCGAAGCAAATATGAA
AACCGCACAGACCGAGTCGCAGCAGCAGCAAATAGGACTGGTGTCATACTTGGTAGACAACAAGCTGGCCGACGCCTATC
ACTTGGCACAAATGCTTTCACAAGCTTTTGGCGATCCTTTGTTTGATCTAGACGCTCTGAGCCTCGATGTCATCCCTAAA
GACTTGGTCGATGAAAAAATCGTCCGTAAGTTTAATGCCTTGCCTTTGTTTAAGCGCGGCCAGCGTTTGTTTGTCGCCTT
AAGTGATCCAACACGTATCGATGCCATTGATGCCATTGCATTTAACTCACGCCTATCTATCGAAACGGTGGTGGTCGAAG
AAGATAAGCTCAAAAAACGCATCGAAGCGCTTTATGCAGACAGTATGCAGGGGCTTGATAGCTTTTCTGATAGCGATTTA
AACGTCGGTTTTGAAGACGGAGATGACGCGGCTGATGAGGGCGAGACCAAGCTCAGTGATAGCGTCGATGAGGCCCCTGT
CGTTAAGTTTGTCAATAAGATGCTGGTGGATGCCATTCGTATGGGAGCTTCAGATCTGCATTTTGAACCCTATGAAAAGA
CCTACCGCGTACGCTTTCGTGTTGATGGGGTCATGCAAAAAATGGCCAGCCCGCCTGTCCAGCTTGCCGGTAAGATCGCA
GCACGCCTAAAGGTCATGTCACAAATGGACATCTCTGAGCGCCGTATCCCGCAAGATGGCCGTATCAAACTAAAACTGTC
AAAAAACAAAGCCATCGACTTTCGGGTAAACTCCTTGCCGACGCTGTTTGGCGAAAAAATCGTACTACGTATCTTGGACC
CCTCATCGGCCATGCTCGGGATTGATGCCTTAGGTTATGAGCCAGATCAGCAAGAGATGTTTTTAGAGGCTCTTCATAAG
CCGCAGGGCATGCTGTTGATCACAGGTCCGACAGGGTCTGGTAAAACCGTATCGCTATATACTGGTATCAACATCCTCAA
CACGGGAGCAACCAATATCTCCACTGCCGAGGATCCAGTAGAGATCAACCTTGAAGGCATCAATCAGGTCAACGTCAACC
CAAAAGTTGGCCTAACCTTTGCCAATGCTTTGAAGTCGTTTTTGCGTCAGGATCCCGATATCGTCATGGTCGGTGAGATT
CGAGATTTAGAGACTGCGGAGATTGCAATAAAAGCGGCGCAAACAGGCCACATGGTACTCTCAACCCTGCACACGAACTC
TGCCCCTGAGACGTTGACGCGTCTACGCAATATGGGGGTGGCTTCTTTTAACATTGCCACCTCTGTCAACTTGGTCATTG
CTCAGCGTTTGGCACGGCGCTTGTGTAAAAACTGCAAAAAAGCCATCAACGTACCGCGGCAAAGTTTGCTTGAGCTTGGC
TTTACTGATGCCGATCTAGACGATCCAGACAACGTCATTTATGAGCCTGTCGGCTGTAGTGAATGCCGTGAAGGCTATAA
AGGCCGAGTGGGTGTTTATGAAGTCATGAAGGTCAGCCCAGACATCTCTCGCATCATCATGGAAGATGGCAATGCCATGG
ATATCAAAGACGCTGCGCTCAAAAACGGCTTTCGCGATCTGCGCCGCTCTGGCATTTTAAAGGTATTACAAGGGGTGACC
AGTATTCAAGAGATGATGCGAGTAACCTCTGAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(193-460)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

67.719

99.825

0.676
  pilB Acinetobacter baylyi ADP1

64.437

99.475

0.641
  pilB Legionella pneumophila strain ERS1305867

51.742

100

0.52
  pilF Neisseria gonorrhoeae MS11

49.912

98.949

0.494
  pilB Vibrio cholerae strain A1552

50.841

93.695

0.476
  pilB Vibrio parahaemolyticus RIMD 2210633

50.187

93.87

0.471
  pilB Vibrio campbellii strain DS40M4

47.653

97.023

0.462
  pilB/pilB1 Synechocystis sp. PCC 6803

39.504

91.769

0.363

Multiple sequence alignment