Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   AADH33_RS12620 Genome accession   NZ_CP151283
Coordinates   3043861..3044763 (+) Length   300 a.a.
NCBI ID   WP_348549109.1    Uniprot ID   A0AAU6RZV5
Organism   Psychrobacter sp. KFRI-CH2-11     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3038861..3049763
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AADH33_RS12605 (AADH33_12595) tpiA 3039366..3040175 (-) 810 WP_348549107.1 triose-phosphate isomerase -
  AADH33_RS12610 (AADH33_12600) pilB 3040526..3042241 (+) 1716 WP_348549108.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AADH33_RS12615 (AADH33_12605) pilC 3042612..3043835 (+) 1224 WP_075101362.1 type II secretion system F family protein Machinery gene
  AADH33_RS12620 (AADH33_12610) pilD 3043861..3044763 (+) 903 WP_348549109.1 prepilin peptidase Machinery gene
  AADH33_RS12625 (AADH33_12615) coaE 3044939..3045625 (+) 687 WP_348549110.1 dephospho-CoA kinase -
  AADH33_RS12630 (AADH33_12620) rlmB 3045588..3046358 (-) 771 WP_100748650.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  AADH33_RS12635 (AADH33_12625) - 3046626..3046961 (-) 336 WP_075101358.1 pyrimidine/purine nucleoside phosphorylase -
  AADH33_RS12640 (AADH33_12630) recN 3047579..3049258 (+) 1680 WP_348549111.1 DNA repair protein RecN -

Sequence

Protein


Download         Length: 300 a.a.        Molecular weight: 33017.53 Da        Isoelectric Point: 8.7024

>NTDB_id=976085 AADH33_RS12620 WP_348549109.1 3043861..3044763(+) (pilD) [Psychrobacter sp. KFRI-CH2-11]
MQFIQLLQDNTIMALVVFGLLGLCVGSFLNVVIHRLPLMMFASWRQECSQFLAEQADMPREHTQPLTRIVAGDSAITLSR
PASRCPHCAHKITWYQNIPLISWLMLRGRCAACNAAIGIRYPLVELITALLSVLIIYQFGVSATGLSALVLVWTLIALTG
IDFDTQLLPDRLTFPLAGLGLAVNSQGWFVSPSQSIWGLLLGFLSLWVVVKLFYLITKKHGMGQGDFKLLAVLGAWLGPM
MLPLIILLSSLLGSIVGLVLMKKQGESRPFAFGPYIAIAGIVALLYGTDIVSWYLGMYTY

Nucleotide


Download         Length: 903 bp        

>NTDB_id=976085 AADH33_RS12620 WP_348549109.1 3043861..3044763(+) (pilD) [Psychrobacter sp. KFRI-CH2-11]
ATGCAATTTATACAGTTATTACAAGACAATACGATTATGGCTTTGGTCGTATTTGGGTTATTAGGGCTTTGTGTCGGTAG
TTTTTTAAATGTGGTCATTCATCGGCTCCCGCTGATGATGTTTGCAAGCTGGCGACAAGAATGTAGTCAGTTTCTGGCTG
AGCAAGCAGACATGCCGCGCGAGCATACGCAGCCACTGACCCGTATCGTCGCAGGCGACAGCGCGATTACCTTGAGTCGG
CCGGCATCGCGCTGCCCGCACTGCGCTCATAAAATCACCTGGTATCAAAACATCCCTTTGATCAGTTGGCTGATGCTGCG
CGGGCGCTGCGCTGCTTGTAACGCCGCCATCGGTATTCGCTATCCACTGGTGGAGCTGATCACTGCCCTACTCTCTGTGC
TGATCATTTATCAATTTGGGGTGAGTGCAACAGGCTTATCGGCGTTGGTCTTAGTATGGACGCTAATCGCCTTAACCGGT
ATCGATTTTGATACTCAGCTCCTCCCTGATCGCTTAACCTTCCCTTTGGCGGGGCTAGGTCTAGCAGTCAACAGTCAAGG
CTGGTTTGTCTCACCCAGCCAGTCGATTTGGGGGTTGCTGTTGGGGTTTTTGTCGTTATGGGTGGTGGTAAAGCTGTTTT
ATTTGATCACCAAAAAACACGGCATGGGTCAGGGCGACTTTAAGTTGTTGGCCGTATTGGGCGCTTGGCTGGGACCTATG
ATGCTACCGCTGATCATCTTACTCTCCTCCCTGCTAGGCTCCATCGTCGGGCTGGTCTTAATGAAAAAACAAGGCGAGAG
CAGACCCTTTGCCTTCGGCCCTTACATTGCTATTGCCGGTATAGTCGCCTTATTATATGGCACAGATATCGTCAGTTGGT
ACTTAGGCATGTATACTTACTAA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(149-258)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(20-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Acinetobacter nosocomialis M2

55.102

98

0.54
  pilD Acinetobacter baumannii D1279779

54.422

98

0.533
  pilD Vibrio campbellii strain DS40M4

48.78

95.667

0.467
  pilD Vibrio cholerae strain A1552

49.462

93

0.46
  pilD Neisseria gonorrhoeae MS11

43.214

93.333

0.403

Multiple sequence alignment