Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   WMB06_RS20185 Genome accession   NZ_CP151275
Coordinates   4435204..4436922 (+) Length   572 a.a.
NCBI ID   WP_341676343.1    Uniprot ID   -
Organism   Niveibacterium sp. SC-1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4430204..4441922
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WMB06_RS20170 (WMB06_20170) ffh 4431561..4432919 (-) 1359 WP_341676340.1 signal recognition particle protein -
  WMB06_RS20175 (WMB06_20175) ccsA 4432973..4433788 (+) 816 WP_341676341.1 cytochrome c biogenesis protein CcsA -
  WMB06_RS20180 (WMB06_20180) - 4433843..4435114 (+) 1272 WP_341676342.1 CNNM domain-containing protein -
  WMB06_RS20185 (WMB06_20185) pilB 4435204..4436922 (+) 1719 WP_341676343.1 type IV-A pilus assembly ATPase PilB Machinery gene
  WMB06_RS20190 (WMB06_20190) pilC 4436939..4438177 (+) 1239 WP_341676344.1 type II secretion system F family protein Machinery gene
  WMB06_RS20195 (WMB06_20195) pilD 4438229..4439080 (+) 852 WP_341676345.1 A24 family peptidase Machinery gene
  WMB06_RS20200 (WMB06_20200) - 4439179..4439454 (+) 276 WP_341676346.1 zinc ribbon domain-containing protein -
  WMB06_RS20205 (WMB06_20205) - 4439475..4440104 (+) 630 WP_341676347.1 DUF502 domain-containing protein -

Sequence

Protein


Download         Length: 572 a.a.        Molecular weight: 62010.21 Da        Isoelectric Point: 5.0571

>NTDB_id=976058 WMB06_RS20185 WP_341676343.1 4435204..4436922(+) (pilB) [Niveibacterium sp. SC-1]
MVANPQQMPLSGLARALVQQKLLSEQQAAELSEQARTSNKPFAAELTQRGVMSARELARVCSEAFGIAMLDLAAFDDTQI
PRDAIDRKLVGTHLIVPLAKRNNKLSIGLADPTNLRALDEVRFQTGLAVEPLIVEIDKLLVLTAKLSESTTDSLKNLTGN
LDEELGEVASGEEGAGDDGASADVDDAPVVKFIQKILVDAINEGASDIHFEPYEKYYRIRFRTDGILREISQPPLVLREK
IAARIKVISRLDISEKRVPQDGRMKLKLSATKSIDFRVSTLPTLHGEKIVMRILDPSSAMLGVDALGYEPAQKRALLDAV
ERPYGMVLVTGPTGSGKTVSLYTCLNLLNKPGVNISTAEDPCEINLPGINQVNVNDKAGLTFAAALKSFLRQDPDVIMVG
EIRDLETAEISIKAAQTGHMVLSTLHTNDAPTTLERLRNMGVAPFNIASSVIMITAQRLARRLCNCKKPLDIPIEALLQA
GYSDEDLDGSWEPMGPGGCDRCKGTGYKGRVGIYQVMPVDEDIAHIIMTGGNSIDIAAEAQRQGINDLRQSGLLKVRQGM
TSLAEVLATTND

Nucleotide


Download         Length: 1719 bp        

>NTDB_id=976058 WMB06_RS20185 WP_341676343.1 4435204..4436922(+) (pilB) [Niveibacterium sp. SC-1]
ATGGTTGCAAATCCTCAGCAGATGCCGCTCAGCGGCTTGGCCCGCGCGCTGGTGCAGCAAAAACTGCTGTCCGAACAACA
GGCGGCCGAGCTTTCCGAGCAGGCGCGGACCTCGAACAAGCCTTTCGCCGCCGAACTCACTCAGCGCGGCGTGATGAGTG
CGCGAGAGCTCGCGCGCGTCTGCTCCGAGGCCTTCGGCATCGCGATGCTGGACTTGGCGGCTTTCGACGATACGCAGATC
CCGCGTGACGCCATCGATCGCAAACTGGTCGGCACGCACCTGATCGTCCCGCTGGCCAAGCGCAACAACAAGCTCTCCAT
CGGCCTCGCGGACCCGACGAACCTGCGCGCACTGGACGAAGTCCGCTTCCAGACCGGTCTGGCCGTCGAACCCTTGATCG
TCGAGATCGACAAGCTGCTGGTCCTGACGGCCAAGCTCTCTGAAAGCACTACCGACTCGCTCAAGAACCTCACAGGCAAC
CTCGACGAAGAACTTGGCGAGGTCGCCAGCGGCGAGGAAGGCGCGGGCGACGATGGCGCGTCCGCCGATGTCGACGATGC
CCCAGTAGTCAAGTTCATCCAGAAGATCCTGGTCGACGCCATCAACGAGGGCGCCTCCGACATCCACTTCGAGCCCTACG
AAAAGTATTACCGGATCCGCTTTCGCACTGACGGCATTCTGCGGGAGATTTCCCAGCCGCCCTTGGTCCTGCGCGAGAAG
ATCGCGGCCCGGATCAAGGTGATCTCCCGGCTGGACATTTCGGAAAAACGTGTCCCGCAGGACGGCCGCATGAAGCTCAA
GCTCTCGGCGACCAAGTCGATCGATTTCCGGGTCAGCACGCTCCCCACGCTGCACGGCGAGAAAATCGTGATGCGTATTC
TCGACCCGAGCTCCGCCATGCTCGGCGTCGACGCGCTCGGCTACGAGCCAGCCCAGAAGAGGGCCCTGCTCGACGCCGTC
GAGCGCCCCTACGGCATGGTTTTGGTCACCGGCCCGACGGGTTCGGGCAAGACGGTGTCGCTCTACACCTGCCTCAACCT
GCTGAACAAGCCTGGCGTCAATATTTCGACGGCAGAGGACCCTTGCGAAATCAACCTGCCCGGCATCAACCAGGTTAACG
TGAATGACAAGGCCGGCCTGACCTTCGCCGCCGCTCTGAAATCCTTCCTGCGCCAGGATCCGGACGTGATCATGGTTGGC
GAAATCCGCGACCTCGAAACCGCTGAGATTTCGATCAAGGCCGCGCAGACCGGCCACATGGTGCTCTCCACCCTGCACAC
CAATGACGCGCCAACCACGCTCGAACGCCTGCGCAACATGGGTGTGGCGCCGTTCAACATCGCGTCGTCGGTCATCATGA
TCACCGCACAACGCCTGGCCCGACGCCTATGCAACTGCAAGAAGCCGCTCGACATTCCGATCGAGGCGCTCCTCCAGGCA
GGCTACTCCGACGAGGACCTGGACGGCAGTTGGGAGCCCATGGGTCCCGGCGGCTGCGACCGCTGCAAGGGAACCGGCTA
TAAGGGACGCGTGGGCATCTATCAGGTCATGCCAGTCGATGAAGACATCGCCCATATAATCATGACGGGCGGCAATTCCA
TTGACATTGCCGCGGAAGCCCAACGCCAGGGAATCAACGATCTGCGTCAGTCAGGACTGCTCAAAGTCCGACAAGGCATG
ACCTCGCTCGCCGAAGTGCTTGCGACCACCAACGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(195-462)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.556

99.126

0.551
  pilB Acinetobacter baylyi ADP1

54.064

98.951

0.535
  pilF Neisseria gonorrhoeae MS11

53.369

98.601

0.526
  pilB Legionella pneumophila strain ERS1305867

50.432

100

0.51
  pilB Vibrio cholerae strain A1552

48.596

99.65

0.484
  pilB Vibrio campbellii strain DS40M4

48.043

98.252

0.472
  pilB Vibrio parahaemolyticus RIMD 2210633

48.208

97.552

0.47
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.566

92.657

0.376
  pilF Thermus thermophilus HB27

37.566

99.126

0.372
  pilB/pilB1 Synechocystis sp. PCC 6803

40.546

89.685

0.364

Multiple sequence alignment