Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AABM54_RS22065 Genome accession   NZ_CP150918
Coordinates   4924474..4926174 (+) Length   566 a.a.
NCBI ID   WP_347902072.1    Uniprot ID   -
Organism   Pseudomonas purpurea strain DGS26     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4919474..4931174
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AABM54_RS22045 - 4919743..4922691 (+) 2949 WP_347902067.1 DUF748 domain-containing protein -
  AABM54_RS22050 - 4922692..4922988 (+) 297 WP_347902068.1 DUF2845 domain-containing protein -
  AABM54_RS22055 - 4923162..4923518 (-) 357 WP_347902070.1 BON domain-containing protein -
  AABM54_RS22060 - 4923832..4924248 (-) 417 WP_347902071.1 pilin -
  AABM54_RS22065 pilB 4924474..4926174 (+) 1701 WP_347902072.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AABM54_RS22070 pilC 4926178..4927395 (+) 1218 WP_347902073.1 type II secretion system F family protein Machinery gene
  AABM54_RS22075 pilD 4927398..4928270 (+) 873 WP_347902074.1 A24 family peptidase Machinery gene
  AABM54_RS22080 coaE 4928267..4928890 (+) 624 WP_347902075.1 dephospho-CoA kinase -
  AABM54_RS22085 yacG 4928887..4929087 (+) 201 WP_347902076.1 DNA gyrase inhibitor YacG -
  AABM54_RS22090 - 4929097..4929312 (-) 216 WP_347902077.1 hypothetical protein -
  AABM54_RS22095 - 4929402..4930091 (-) 690 WP_347902078.1 energy-coupling factor ABC transporter permease -
  AABM54_RS22100 - 4930359..4930985 (+) 627 WP_347902079.1 DUF1780 domain-containing protein -
  AABM54_RS22105 - 4931033..4931150 (+) 118 Protein_4340 MOSC domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62589.55 Da        Isoelectric Point: 5.4223

>NTDB_id=973783 AABM54_RS22065 WP_347902072.1 4924474..4926174(+) (pilB) [Pseudomonas purpurea strain DGS26]
MNDIALSGLAKQLVLAEVLTEKSAQQAYQHAQRSRISLASYLVQNKLAKSRQVAEIASEHFGVAFLDLNCLDKETQPTGL
VSEKLVRQHHALPLWRRGNKLFVGISDPSNHQAINDIQFSTGLTTEAILVEDDKLTDAIERFFDSHTTGLEDMADVDLDS
LDIETIDDRRQDAIAGQDADDAPVVRFVNKMLLDAIKGGASDLHFEPYEKTYRVRVRTDGLLCEVAKPPIQLANRIAARL
KVMASLDISERRKPQDGRIKMRLSNSKSIDFRVNTLPTLWGEKIVIRILDPSSAQIGIDALGYEPEQKDLYMAALKQPQG
LILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPKQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVLSTLHTNSAAETLTRLHNMGIPGFNIATSVNLIIAQRLARKLCSHCKQAIDIPEETLLKEGFPR
ERIGSFTIYEPVGCELCIGGYKGRVGIYEVVKNTPELQRLIMAEGNSLEIDTQMRKDGFNDLRTSGLLKAMQGVTSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=973783 AABM54_RS22065 WP_347902072.1 4924474..4926174(+) (pilB) [Pseudomonas purpurea strain DGS26]
ATGAATGACATCGCCCTGTCCGGCCTGGCCAAGCAATTGGTGCTGGCCGAAGTGCTCACTGAAAAAAGCGCGCAACAGGC
GTATCAGCACGCTCAACGCAGCCGGATTTCCCTCGCCAGCTACTTGGTACAGAACAAACTGGCGAAAAGTCGTCAGGTCG
CGGAAATCGCTTCGGAACATTTCGGCGTTGCCTTCCTGGACCTGAACTGCCTCGACAAGGAAACCCAGCCCACTGGCCTG
GTCAGCGAAAAACTGGTTCGCCAACACCATGCCCTGCCCCTCTGGCGGCGTGGCAACAAGTTGTTCGTCGGTATCTCCGA
CCCTAGCAACCACCAGGCCATCAACGACATCCAGTTCAGCACCGGGCTGACCACCGAAGCCATCCTGGTGGAGGACGACA
AACTCACTGACGCCATTGAGCGATTCTTCGACAGCCACACCACCGGCCTTGAAGATATGGCCGACGTGGACCTGGATAGC
CTGGACATCGAAACAATCGACGACAGGCGCCAGGATGCCATCGCCGGGCAGGACGCCGACGACGCGCCGGTGGTGCGCTT
CGTCAACAAGATGCTGCTCGATGCGATCAAGGGCGGGGCCTCGGACCTGCATTTCGAGCCCTACGAAAAAACCTATCGGG
TACGGGTGCGTACCGACGGTCTGCTGTGCGAAGTGGCCAAGCCGCCGATCCAGTTGGCCAACCGTATCGCTGCGCGACTC
AAAGTCATGGCCAGCCTGGATATCTCGGAGCGCCGCAAGCCTCAGGACGGGCGCATCAAAATGCGCCTGTCGAACAGCAA
ATCCATCGACTTTCGGGTCAACACCCTGCCAACACTGTGGGGCGAAAAAATCGTCATCCGGATTCTCGACCCGTCCAGCG
CCCAAATAGGCATCGATGCCCTCGGCTACGAACCCGAGCAAAAAGACCTGTACATGGCGGCGCTCAAGCAGCCCCAAGGG
TTGATCCTGGTAACCGGGCCGACCGGCTCGGGCAAAACCGTCTCGCTGTACACCGGCCTGAACATCCTCAACACCGTCGA
CATCAACATCTCCACCGCCGAAGACCCGGTGGAAATCAACATGGAAGGCATCAACCAGGTCAACGTCAATCCCAAGCAAG
GACTGGACTTCGCCCAGGCGCTACGCTCCTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGTGAGATTCGTGACCTG
GAAACCGCCGAAATCGCCATCAAAGCCGCGCAAACCGGGCACATGGTGCTTTCCACCCTGCACACCAACAGCGCCGCGGA
AACCCTGACCCGGCTGCACAACATGGGCATTCCAGGGTTCAACATCGCTACTTCGGTCAACCTCATCATCGCCCAGCGCC
TGGCGCGCAAACTGTGCAGCCACTGCAAGCAAGCCATCGACATTCCCGAAGAGACACTGCTCAAGGAAGGATTCCCTCGG
GAACGCATCGGCTCGTTCACGATCTATGAGCCCGTCGGTTGCGAACTCTGCATTGGCGGCTACAAAGGACGCGTGGGAAT
CTATGAAGTGGTGAAAAACACCCCCGAGCTGCAACGGTTGATCATGGCCGAAGGCAATTCGCTGGAGATCGACACGCAAA
TGCGCAAGGACGGCTTCAACGACCTGCGCACTTCAGGTCTGCTCAAAGCCATGCAAGGCGTTACCAGCCTTGAAGAAATC
AACCGGGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.865

99.647

0.587
  pilB Acinetobacter baumannii D1279779

58.688

99.647

0.585
  pilB Legionella pneumophila strain ERS1305867

55.83

100

0.558
  pilB Vibrio cholerae strain A1552

50.619

99.823

0.505
  pilB Vibrio campbellii strain DS40M4

48.772

100

0.491
  pilB Vibrio parahaemolyticus RIMD 2210633

49.82

98.233

0.489
  pilF Neisseria gonorrhoeae MS11

48.845

99.47

0.486
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.611

99.117

0.373
  pilF Thermus thermophilus HB27

36.572

100

0.366

Multiple sequence alignment