Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AABM55_RS12010 Genome accession   NZ_CP150917
Coordinates   2614269..2615387 (+) Length   372 a.a.
NCBI ID   WP_347929676.1    Uniprot ID   -
Organism   Pseudomonas helvetica strain DGS28     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2609269..2620387
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AABM55_RS11995 - 2610264..2611199 (+) 936 WP_347929674.1 sugar kinase -
  AABM55_RS12000 - 2611258..2611533 (-) 276 WP_019690952.1 peptidylprolyl isomerase -
  AABM55_RS12005 - 2611587..2614124 (-) 2538 WP_347929675.1 PAS domain-containing protein -
  AABM55_RS12010 pilU 2614269..2615387 (+) 1119 WP_347929676.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AABM55_RS12015 - 2615407..2617362 (-) 1956 WP_347929677.1 acetoacetate--CoA ligase -
  AABM55_RS12020 - 2617554..2618327 (-) 774 WP_054596812.1 3-hydroxybutyrate dehydrogenase -
  AABM55_RS12025 - 2618335..2619726 (-) 1392 WP_347929678.1 GntP family permease -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41525.40 Da        Isoelectric Point: 6.1946

>NTDB_id=973755 AABM55_RS12010 WP_347929676.1 2614269..2615387(+) (pilU) [Pseudomonas helvetica strain DGS28]
MDIHSLLRMLASQDGSDLYLSTGAPPTGRFKGALRTLAHEVFKPGEIARIAETLMDVEQRAEFDRELEMNLAVSLVGVGR
FRVNIFKQRNDVSIVARNIKLDIPRFEDLKLPAVLLETVMLKQGLMLFVGATDSGKSTSLAALIDYRNRHSSGHIITIED
PVEYIHSHQQSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALAFADTGHLVLSTLHAHNASQALDRI
INFFPEERRGQLLNDLGNNLKAFVSQRLVRTRDGQRRAAVEVMLGTPTIGDLIRRNELHQLKEIMEKSAESGMKTFDTAL
FDLVVEGAIDEEEALKFADSVNNLRLRLKLHADSVPNAAPPSTETGEWGLMD

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=973755 AABM55_RS12010 WP_347929676.1 2614269..2615387(+) (pilU) [Pseudomonas helvetica strain DGS28]
ATGGACATTCACTCGCTGCTAAGAATGCTGGCCAGCCAGGATGGCTCCGATCTTTACCTGTCGACTGGCGCACCGCCTAC
CGGGCGTTTCAAGGGTGCGCTCCGAACACTGGCCCATGAAGTGTTCAAGCCCGGCGAAATCGCCCGGATTGCCGAGACCC
TTATGGACGTCGAGCAGCGTGCAGAGTTCGACCGCGAGCTGGAAATGAACCTGGCGGTTTCTCTGGTTGGCGTCGGGCGC
TTTCGCGTCAACATCTTCAAGCAGCGCAATGACGTGTCGATCGTGGCGCGCAACATCAAACTCGATATCCCGCGCTTTGA
AGACCTTAAACTACCCGCCGTGCTGCTTGAGACGGTGATGCTCAAACAGGGACTGATGTTGTTCGTCGGCGCTACGGATT
CGGGCAAGTCGACATCGCTGGCAGCGTTGATCGATTACCGCAATCGCCACAGCAGCGGGCACATCATCACCATCGAAGAC
CCGGTGGAGTATATCCATAGCCATCAACAGTCGATCATCAACCAGCGTGAGGTGGGCGTCGATACGCGCAGCTTTCATGC
CGCCTTGAAGAACACCCTGCGTCAGGCCCCGGATGTGGTGCTGATCGGTGAAATTCGCGACCGCGAAACCATGGAGCATG
CATTGGCTTTCGCCGACACCGGGCACCTGGTGCTCTCCACCTTGCACGCCCACAACGCCAGTCAGGCGCTGGACCGGATC
ATCAACTTTTTCCCGGAAGAACGCCGTGGGCAGTTGCTCAATGATCTGGGTAACAACCTCAAGGCCTTTGTTTCCCAACG
TCTGGTACGCACCCGCGATGGGCAGCGCCGGGCGGCGGTGGAGGTGATGCTGGGTACGCCAACCATTGGCGATCTGATAC
GGCGCAATGAGCTTCACCAGCTCAAGGAGATCATGGAAAAGTCGGCAGAGTCGGGAATGAAGACGTTTGATACGGCGTTG
TTCGATTTGGTTGTCGAGGGCGCGATCGATGAAGAAGAGGCGTTGAAATTCGCCGATTCGGTGAATAACTTGCGCTTGCG
CCTGAAGTTGCACGCGGACAGTGTGCCGAATGCCGCCCCCCCATCCACCGAAACCGGTGAATGGGGGCTGATGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

54.648

95.43

0.522
  pilU Acinetobacter baylyi ADP1

51.705

94.624

0.489
  pilU Vibrio cholerae strain A1552

50.857

94.086

0.478
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.887

100

0.395
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.742

89.516

0.374
  pilT Vibrio cholerae strain A1552

41.742

89.516

0.374
  pilT Pseudomonas stutzeri DSM 10701

38.953

92.473

0.36

Multiple sequence alignment