Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   AABM55_RS01930 Genome accession   NZ_CP150917
Coordinates   431245..433326 (+) Length   693 a.a.
NCBI ID   WP_347928678.1    Uniprot ID   -
Organism   Pseudomonas helvetica strain DGS28     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 426245..438326
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AABM55_RS01910 comM 428457..429521 (+) 1065 WP_347928675.1 pilus assembly protein PilM Machinery gene
  AABM55_RS01915 - 429521..430087 (+) 567 WP_103317115.1 PilN domain-containing protein -
  AABM55_RS01920 pilO 430084..430707 (+) 624 WP_347928676.1 type 4a pilus biogenesis protein PilO -
  AABM55_RS01925 - 430704..431231 (+) 528 WP_347928677.1 pilus assembly protein PilP -
  AABM55_RS01930 pilQ 431245..433326 (+) 2082 WP_347928678.1 type IV pilus secretin PilQ Machinery gene
  AABM55_RS01935 aroK 433331..433849 (+) 519 WP_054595232.1 shikimate kinase AroK -
  AABM55_RS01940 aroB 433997..435097 (+) 1101 WP_054595233.1 3-dehydroquinate synthase -
  AABM55_RS01945 - 435108..436709 (+) 1602 WP_103317123.1 AAA family ATPase -

Sequence

Protein


Download         Length: 693 a.a.        Molecular weight: 75004.35 Da        Isoelectric Point: 6.5842

>NTDB_id=973743 AABM55_RS01930 WP_347928678.1 431245..433326(+) (pilQ) [Pseudomonas helvetica strain DGS28]
MNRIFSTLGFSLWMALLSPMVQAANLKALDVAALPGDRIELKLAFDGPPPAPHGYTTDQPARIALDLPGVSSQLASKTRD
LGGGNARSATVVEAKDRTRLIINLTQLTPYSTRVEGNTLFVVVGQAAAAQKPRSAAPRVASAMPVAAKTYAPVSKAIRGV
DFQRGAQGEGNVVIDLSDPGISPDIQERDGKIILTFAKTQLPERLRVRLDVKDFATPVQFVNASASADKASISIEPGGTY
DYSTYQTDNKLTVSVRPMTVDDLQKRNADRFAYSGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQNVP
WDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESRKQIAELAPLRRELLQVNYAKAADIAKLFQSVTSAEAKV
DERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSVRNKGNWNASGVSNG
ANASSTIGTPGSTSTNSPFVDMGAAGNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIP
YQEASSSGATSVAFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFS
NTQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSH

Nucleotide


Download         Length: 2082 bp        

>NTDB_id=973743 AABM55_RS01930 WP_347928678.1 431245..433326(+) (pilQ) [Pseudomonas helvetica strain DGS28]
ATGAACAGGATCTTCTCAACCCTCGGTTTTTCGCTATGGATGGCGCTGCTTTCTCCGATGGTGCAGGCAGCCAATCTCAA
AGCGCTGGATGTCGCCGCGCTGCCGGGTGATCGCATCGAGTTGAAACTGGCTTTCGACGGGCCACCGCCCGCGCCTCATG
GCTATACCACCGATCAGCCGGCACGCATCGCCCTTGATCTGCCGGGTGTGAGCAGCCAACTGGCGAGCAAGACGCGGGAT
CTGGGAGGCGGCAACGCCCGCAGTGCGACAGTGGTCGAGGCCAAGGACCGCACCCGTTTGATCATCAACCTGACGCAGTT
GACGCCTTACAGCACGCGAGTCGAGGGCAATACGCTGTTCGTGGTGGTTGGGCAGGCTGCTGCCGCTCAGAAACCCCGAT
CCGCGGCACCGCGAGTGGCGAGCGCCATGCCAGTGGCCGCCAAGACTTATGCTCCCGTGAGCAAGGCCATTCGCGGCGTG
GACTTTCAGCGCGGTGCGCAGGGTGAAGGCAATGTCGTCATTGATTTGTCAGACCCTGGTATCAGTCCGGACATCCAGGA
GCGTGATGGCAAGATCATCCTCACATTCGCCAAAACCCAGTTGCCTGAACGGTTGCGGGTTCGCCTGGACGTCAAGGATT
TCGCCACGCCGGTGCAGTTCGTCAATGCCAGTGCGTCCGCCGATAAAGCGAGCATCAGCATCGAGCCCGGTGGCACATAC
GACTATTCGACCTACCAGACCGACAACAAGCTGACCGTCAGCGTCCGCCCGATGACTGTCGACGATCTGCAGAAGCGCAA
CGCAGATCGTTTCGCCTACAGCGGTGAGAAGCTGTCGTTGAATTTTCAGGACATCGATGTGCGCTCGGTGCTGCAACTGA
TCGCCGACTTTACCAACCTCAACCTGGTGGCCAGCGACACGGTGCAGGGCGGCATCACCTTGCGCCTGCAAAACGTGCCG
TGGGATCAGGCGCTGGATCTGGTGCTCAAGACCAAAGGGTTGGACAAGCGCAAGATCGGTAACGTCTTGCTGGTCGCGCC
GGCAGATGAGATTGCCGCCCGCGAGCGCCAGGAGCTGGAGTCGCGCAAACAGATCGCCGAGCTGGCGCCACTGCGCCGTG
AGCTGTTGCAGGTCAACTACGCCAAGGCGGCGGATATCGCCAAGCTGTTCCAGTCGGTGACCAGCGCCGAGGCGAAAGTC
GACGAGCGTGGTTCGATCACTGTCGACGAGCGAACCAACAATATCATCGCCTACCAGACCCAGGATCGCCTGGATGAGCT
ACGGCGAATCGTCGCTCAGCTGGATATTCCGGTACGCCAGGTGATGATCGAGGCGCGGATCGTCGAAGCCAACGTCGACT
ACGACAAAAGCCTCGGCGTGCGTTGGGGCGGCTCGGTCCGCAACAAGGGTAACTGGAATGCCTCCGGGGTCAGCAACGGG
GCCAACGCCTCATCGACCATTGGTACACCGGGCAGCACCAGCACCAACTCACCGTTCGTCGATATGGGCGCGGCAGGGAA
TACATCGGGGATCGGCATCGCCTTCATCACCGATAACGTGTTGCTGGACCTTGAGCTGACGGCCATGGAGAAGACCGGTA
ACGGGGAAATCGTCTCTCAACCCAAAGTGGTCACCTCCGACAAGGAAACCGCGAAGATCCTCAAGGGTACGGAAATCCCC
TATCAGGAAGCCAGCTCCAGCGGCGCGACATCGGTGGCGTTCAAGGAGGCCTCGCTGTCTCTGGAGGTGACGCCGCAGAT
CACCCCGGATAACCGCATCATCATGGAGGTCAAGGTCACCAAGGATGAGCCGGACTACCTGAACAAAGTGCAGGATGTGC
CGCCGATCAAGAAGAACGAGGTCAATGCCAAGGTACTGGTCAACGATGGCGAAACCATCGTCATTGGCGGGGTTTTCTCA
AATACTCAGAGCAAGGTTGTAGATAAGGTGCCATTTCTCGGTGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGACGT
GGTGTCGGAGAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGAGTCATT
GA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(375-440)

AMIN domain (AMIN)

(37-122)

Bacterial type II and III secretion system protein (Secretin)

(528-684)

Secretin and TonB N terminus short domain (STN)

(301-347)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.427

100

0.758
  comQ Acinetobacter baylyi ADP1

40.329

100

0.424
  pilQ Acinetobacter baumannii strain A118

37.849

100

0.411
  pilQ Acinetobacter baumannii D1279779

37.849

100

0.411
  pilQ Legionella pneumophila strain ERS1305867

38.609

97.547

0.377
  pilQ Legionella pneumophila strain Lp02

38.462

97.547

0.375

Multiple sequence alignment