Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   VUK15_RS07400 Genome accession   NZ_CP150462
Coordinates   391765..393456 (-) Length   563 a.a.
NCBI ID   WP_340642185.1    Uniprot ID   -
Organism   Photobacterium damselae subsp. piscicida strain NH-LM2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 386765..398456
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VUK15_RS07370 (VUK15_07370) mutT 387118..387525 (-) 408 WP_069530749.1 8-oxo-dGTP diphosphatase MutT -
  VUK15_RS07375 (VUK15_07375) yacG 387786..387995 (-) 210 WP_005297592.1 DNA gyrase inhibitor YacG -
  VUK15_RS07380 (VUK15_07380) zapD 388004..388741 (-) 738 WP_005297594.1 cell division protein ZapD -
  VUK15_RS07385 (VUK15_07385) coaE 388809..389411 (-) 603 WP_151182736.1 dephospho-CoA kinase -
  VUK15_RS07390 (VUK15_07390) pilD 389413..390291 (-) 879 WP_340642184.1 prepilin peptidase Machinery gene
  VUK15_RS07395 (VUK15_07395) pilC 390473..391699 (-) 1227 WP_068969232.1 type II secretion system F family protein Machinery gene
  VUK15_RS07400 (VUK15_07400) pilB 391765..393456 (-) 1692 WP_340642185.1 type IV-A pilus assembly ATPase PilB Machinery gene
  VUK15_RS07405 (VUK15_07405) - 393456..393878 (-) 423 WP_340642186.1 pilin -
  VUK15_RS07410 (VUK15_07410) ampD 394394..394972 (+) 579 WP_340642187.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  VUK15_RS07415 (VUK15_07415) pdhR 395332..396105 (+) 774 WP_005297609.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 563 a.a.        Molecular weight: 62506.14 Da        Isoelectric Point: 5.2594

>NTDB_id=970369 VUK15_RS07400 WP_340642185.1 391765..393456(-) (pilB) [Photobacterium damselae subsp. piscicida strain NH-LM2]
MHTTLASILHQADLISSVQQQQVEDSVSMEGITVPSALIHLGIFSDRELAQHLSHIFGLTLVSVHDYDFEACCKAVYQRD
LVLRHRALPLAQNADTLFLGVSDPCNIDGQDEFRFATGRNIEPLLLENKQLESAIRRIYGRELGETTNMASRDVSDDDLN
NLVDIGDDELDQTTDLGQENEPVTRYINQVLLDAVRKKASDIHFEPYETSYRIRFRCDGILHQHATPPAHLSRRLSTRLK
VMSRLNIAERRQPQDGRIKLKLTANLAVDLRVSTLPTLWGEKVVLRILDSSSANLDIDILGYNQQQKAAYLNALQQPQGM
ILMTGPTGSGKTVSLYTGLSILNTEERNISTAEDPVEINLPGINQVHINNQAGLGFAEALRSFLRQDPDVVMVGEIRDLE
TASIAVKAAQTGHLVLSTLHTNSAAETIIRLTNMGIEDFNLASSLTLIIAQRLGRRLCPHCKQVDEIDGFMQDKLRIPAG
TTLYKANPEGCDDCNKGYLGRVGIYEVMPFSRRLAQALVDGATTLELENIAVEEGMLTLQQSGIEKLCLGITSLKELQRV
LHF

Nucleotide


Download         Length: 1692 bp        

>NTDB_id=970369 VUK15_RS07400 WP_340642185.1 391765..393456(-) (pilB) [Photobacterium damselae subsp. piscicida strain NH-LM2]
ATGCACACCACTCTCGCCTCGATTCTGCACCAGGCTGATCTGATCAGCTCGGTGCAGCAGCAACAAGTCGAAGACAGTGT
CAGTATGGAGGGAATCACGGTTCCCTCCGCACTGATCCATTTGGGCATATTTAGCGATCGCGAACTGGCCCAACACCTTA
GCCACATATTTGGTCTTACCCTCGTTTCGGTCCATGACTACGATTTTGAAGCTTGCTGTAAAGCGGTCTATCAACGCGAT
CTGGTATTACGCCATCGCGCACTGCCCCTTGCTCAAAATGCGGACACACTGTTTCTAGGGGTCAGCGATCCATGCAATAT
CGACGGACAAGATGAGTTCCGTTTTGCCACCGGCCGTAATATTGAACCGCTACTGCTAGAAAACAAACAGCTCGAATCGG
CTATCCGTCGTATTTATGGTCGCGAGTTGGGCGAAACCACCAATATGGCCAGTCGTGATGTCAGCGATGATGATCTGAAT
AACTTAGTCGATATTGGTGATGATGAGCTGGATCAAACTACAGATCTAGGCCAAGAAAACGAACCTGTTACTCGCTATAT
CAACCAAGTACTGCTCGATGCAGTACGTAAAAAAGCCTCCGATATCCATTTTGAACCATATGAGACCAGCTATCGGATCC
GCTTTCGTTGTGATGGCATCTTACACCAACACGCAACACCACCAGCCCATCTTTCTCGTCGTCTTTCTACCCGACTCAAA
GTGATGTCGCGCCTAAATATTGCCGAACGACGCCAACCACAAGATGGACGCATAAAACTTAAGCTTACAGCCAATTTAGC
CGTAGATCTGCGGGTTTCTACCCTGCCCACTTTATGGGGGGAGAAAGTGGTGCTGCGTATTTTAGACAGCTCCAGTGCCA
ACTTAGACATTGATATTTTAGGTTACAACCAGCAACAAAAAGCCGCTTATCTTAATGCACTGCAACAGCCGCAAGGGATG
ATCTTAATGACAGGGCCAACTGGCAGCGGCAAAACCGTTTCGCTTTATACCGGCCTTAGCATTTTAAATACCGAAGAGCG
CAATATTTCTACCGCTGAAGATCCGGTAGAGATCAACTTACCCGGCATTAATCAGGTTCACATTAACAATCAAGCTGGCC
TCGGATTTGCTGAAGCGCTGCGCTCATTTTTACGTCAAGACCCTGATGTGGTGATGGTAGGTGAAATCCGCGATCTGGAA
ACCGCCTCTATTGCCGTTAAAGCTGCGCAAACTGGGCACTTAGTACTATCGACCTTACACACCAATTCTGCCGCCGAAAC
CATTATCCGCCTAACCAATATGGGCATTGAAGACTTTAACCTAGCCTCATCGCTAACACTGATTATTGCTCAGCGCTTAG
GTCGCCGGCTCTGCCCTCACTGTAAACAAGTAGATGAGATTGACGGCTTTATGCAAGACAAACTGCGCATTCCGGCTGGC
ACTACCTTGTATAAAGCCAATCCAGAAGGGTGTGATGACTGCAATAAAGGCTATTTAGGTCGTGTCGGTATCTATGAAGT
GATGCCATTTAGCCGTCGGCTGGCTCAAGCTCTGGTTGATGGCGCGACTACCTTAGAGCTGGAAAATATCGCAGTAGAAG
AAGGGATGCTGACGCTACAACAATCGGGAATAGAAAAGCTCTGTCTTGGCATCACCAGTTTAAAAGAATTACAGCGCGTC
CTGCATTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(189-456)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-143)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

62.167

100

0.622
  pilB Vibrio parahaemolyticus RIMD 2210633

61.279

100

0.613
  pilB Vibrio campbellii strain DS40M4

60.746

100

0.607
  pilB Legionella pneumophila strain ERS1305867

50.184

96.625

0.485
  pilB Acinetobacter baylyi ADP1

49.27

97.336

0.48
  pilB Acinetobacter baumannii D1279779

50.672

92.54

0.469
  pilF Neisseria gonorrhoeae MS11

44.366

100

0.448
  pilB/pilB1 Synechocystis sp. PCC 6803

39.692

92.185

0.366

Multiple sequence alignment