Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   ACLFAG_RS30855 Genome accession   NZ_CP177247
Coordinates   6584730..6586223 (+) Length   497 a.a.
NCBI ID   WP_003098248.1    Uniprot ID   Q9HTR4
Organism   Pseudomonas aeruginosa strain WYT     
Function   require for natural transformation (predicted from homology)   
Unclear

Genomic Context

Location: 6579730..6591223
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLFAG_RS30825 (ACLFAG_30820) - 6579873..6580787 (-) 915 WP_043156737.1 fimbrial protein -
  ACLFAG_RS30830 (ACLFAG_30825) sutA 6581202..6581519 (-) 318 WP_003098240.1 transcriptional regulator SutA -
  ACLFAG_RS30835 (ACLFAG_30830) - 6581597..6582022 (-) 426 WP_003121288.1 secondary thiamine-phosphate synthase enzyme YjbQ -
  ACLFAG_RS30840 (ACLFAG_30835) - 6582283..6583611 (-) 1329 WP_043156738.1 ammonium transporter -
  ACLFAG_RS30845 (ACLFAG_30840) glnK 6583651..6583989 (-) 339 WP_003096476.1 P-II family nitrogen regulator -
  ACLFAG_RS30850 (ACLFAG_30845) - 6584429..6584689 (+) 261 WP_003096478.1 accessory factor UbiK family protein -
  ACLFAG_RS30855 (ACLFAG_30850) comM 6584730..6586223 (+) 1494 WP_003098248.1 YifB family Mg chelatase-like AAA ATPase Machinery gene
  ACLFAG_RS30860 (ACLFAG_30855) betT 6586348..6588333 (+) 1986 WP_003096496.1 choline BCCT transporter BetT -
  ACLFAG_RS30865 (ACLFAG_30860) pchP 6588376..6589425 (-) 1050 WP_003110458.1 phosphorylcholine phosphatase -
  ACLFAG_RS30870 (ACLFAG_30865) - 6589576..6590493 (-) 918 WP_031634368.1 LysR substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 53083.04 Da        Isoelectric Point: 7.9587

>NTDB_id=970190 ACLFAG_RS30855 WP_003098248.1 6584730..6586223(+) (comM) [Pseudomonas aeruginosa strain WYT]
MSLAIVHSRAQVGVEAPCVSVEAHLANGLPSLTLVGLPETAVRESKDRVRSALLNAGFDFPARRITLNLAPADLPKDGGR
FDLAIALGILAASGQLPGTALDGLECLGELALSGAIRPVRGVLPAALAARDARRVLVVPKENAEEASLASGLTVFAVDHL
LEIAGHLSGQAPLPPYQARGLLRAPFPYPDLAEVQGQAAAKRALLVAAAGAHNLLLSGPPGTGKTLLASRLPGLLPALDE
DEALEVAAIHSVASHVPLRHWPQRPFRQPHHSASAPALVGGGSRPQPGEITLAHQGVLFLDELPEFERKVLEVLREPLES
GEIVIARANGRVRFPARFQLVAAMNPCPCGYLGDPSGRCRCTPEQVQRYRGKLSGPLLDRIDLHVSVLRESTSLQPGHGE
TATAEVSERVGAARQRQLARQGCANAHLDLQAMHRNCALAEADRRWLEAAGERLELSLRALHRILKVARTLADLERIDAI
ERRHLAEALQYRAMTST

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=970190 ACLFAG_RS30855 WP_003098248.1 6584730..6586223(+) (comM) [Pseudomonas aeruginosa strain WYT]
ATGTCCCTGGCGATTGTCCACAGCCGAGCCCAGGTCGGCGTCGAAGCCCCCTGCGTCAGCGTCGAGGCGCACCTGGCCAA
CGGCCTGCCTTCGCTGACCCTGGTCGGCCTGCCGGAAACCGCGGTGCGCGAGAGCAAGGACCGCGTGCGCAGCGCCCTGC
TCAATGCCGGTTTCGACTTCCCCGCGCGGCGCATCACCCTCAACCTCGCCCCCGCCGACCTACCCAAGGACGGCGGTCGC
TTCGACCTGGCCATCGCACTCGGCATCCTCGCCGCCAGCGGCCAGTTGCCCGGCACCGCCCTCGACGGCCTGGAGTGCCT
CGGCGAACTGGCCCTGTCCGGGGCGATCCGCCCAGTGCGAGGCGTATTGCCGGCCGCGCTGGCGGCGCGCGACGCAAGGC
GCGTTCTGGTGGTACCGAAGGAAAATGCCGAAGAGGCCAGCCTGGCCAGCGGGCTGACGGTGTTCGCCGTGGACCACCTG
CTGGAGATCGCCGGACACCTCTCCGGCCAGGCCCCGCTGCCGCCCTACCAGGCCCGCGGCCTGCTCCGCGCGCCCTTCCC
TTATCCAGACCTGGCCGAGGTCCAGGGCCAGGCCGCCGCCAAGCGCGCCCTGCTGGTGGCCGCCGCCGGCGCGCACAACC
TGTTGCTCAGCGGCCCGCCGGGCACCGGCAAGACCCTCCTGGCCAGCCGCCTGCCCGGCCTGCTGCCGGCGCTCGACGAG
GACGAAGCCCTGGAGGTCGCGGCGATCCATTCGGTGGCCAGCCACGTCCCCCTCAGGCACTGGCCGCAGCGACCGTTCCG
CCAGCCGCACCACTCCGCCTCCGCGCCGGCCCTGGTCGGCGGCGGCAGCCGCCCGCAGCCGGGCGAGATCACCCTGGCGC
ACCAGGGCGTGCTGTTCCTCGACGAACTGCCGGAGTTCGAGCGCAAGGTCCTGGAGGTCCTGCGTGAGCCGCTGGAAAGC
GGCGAGATCGTCATTGCCCGGGCCAACGGCCGGGTACGTTTCCCGGCGCGCTTCCAACTGGTGGCGGCGATGAATCCCTG
TCCCTGTGGCTACCTCGGCGATCCCAGTGGCCGCTGCCGCTGCACCCCGGAACAGGTCCAGCGCTACCGGGGCAAGCTGT
CCGGACCGCTGCTCGATCGCATCGACCTGCACGTCAGCGTGCTCCGCGAAAGCACCAGCCTGCAGCCAGGACACGGCGAA
ACCGCTACCGCCGAGGTCAGCGAACGGGTGGGCGCCGCACGGCAACGGCAACTGGCCCGCCAGGGCTGCGCCAATGCCCA
TCTCGATCTCCAGGCGATGCACCGCAATTGTGCACTCGCCGAAGCGGACCGCCGCTGGCTGGAGGCTGCCGGAGAGCGCC
TGGAACTTTCCTTGCGCGCCTTGCATCGCATACTCAAGGTGGCCCGGACGCTGGCCGACCTGGAGCGCATCGATGCCATC
GAACGCCGGCACCTGGCGGAAGCCCTGCAGTATCGGGCAATGACCTCCACGTGA

Domains

Predicted by InterProScan.

Subunit ChlI of Mg-chelatase (ChlI)

(21-142)

Magnesium chelatase, subunit ChlI (Mg_chelatase)

(190-390)

Magnesium chelatase, subunit ChlI C-terminal (Mg_chelatase_C)

(402-492)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9HTR4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Haemophilus influenzae Rd KW20

55.186

100

0.567
  comM Vibrio campbellii strain DS40M4

56.74

100

0.567
  comM Vibrio cholerae O1 biovar El Tor strain E7946

56.338

100

0.563
  comM Vibrio cholerae strain A1552

56.338

100

0.563
  comM Glaesserella parasuis strain SC1401

55.179

100

0.557
  comM Acinetobacter baylyi ADP1

53.614

100

0.537
  comM Legionella pneumophila str. Paris

50.1

100

0.503
  comM Legionella pneumophila strain ERS1305867

50.1

100

0.503
  RA0C_RS07335 Riemerella anatipestifer ATCC 11845 = DSM 15868

45.866

100

0.469
  comM Helicobacter pylori 26695

38.845

100

0.392