Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   MKY32_RS06365 Genome accession   NZ_CP150259
Coordinates   1273729..1275558 (+) Length   609 a.a.
NCBI ID   WP_282334326.1    Uniprot ID   -
Organism   Bacillus sp. FSL K6-0998     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 1268729..1280558
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKY32_RS06350 (MKY32_06350) - 1269916..1270632 (-) 717 WP_095266770.1 response regulator -
  MKY32_RS06355 (MKY32_06355) - 1270655..1272250 (-) 1596 WP_009328826.1 sensor histidine kinase -
  MKY32_RS06360 (MKY32_06360) - 1272368..1273516 (+) 1149 WP_095266772.1 competence protein CoiA family protein -
  MKY32_RS06365 (MKY32_06365) pepF 1273729..1275558 (+) 1830 WP_282334326.1 oligoendopeptidase F Regulator
  MKY32_RS06370 (MKY32_06370) - 1275615..1275782 (-) 168 WP_009328823.1 hypothetical protein -
  MKY32_RS06375 (MKY32_06375) spxH 1276194..1277096 (-) 903 WP_098408000.1 protease adaptor protein SpxH -
  MKY32_RS06380 (MKY32_06380) - 1277093..1277491 (-) 399 WP_003180652.1 thiol management oxidoreductase -
  MKY32_RS06385 (MKY32_06385) - 1277773..1278426 (-) 654 WP_009328821.1 lytic transglycosylase domain-containing protein -
  MKY32_RS06390 (MKY32_06390) - 1278439..1279011 (-) 573 WP_009328820.1 CYTH domain-containing protein -
  MKY32_RS06395 (MKY32_06395) - 1279142..1279507 (+) 366 WP_009328819.1 hypothetical protein -
  MKY32_RS06400 (MKY32_06400) - 1279539..1280174 (+) 636 WP_009328818.1 GTP pyrophosphokinase family protein -

Sequence

Protein


Download         Length: 609 a.a.        Molecular weight: 70381.02 Da        Isoelectric Point: 4.8562

>NTDB_id=968370 MKY32_RS06365 WP_282334326.1 1273729..1275558(+) (pepF) [Bacillus sp. FSL K6-0998]
MAEEKKSKKLPSREEVKQEDTWRLEDIFPSDDAWNEEFQAVKELLPKLSEFKGKLGHSADDLYEALTYQDKVMERLGKLY
TYAHMRYDQDTGNSFYQGLNDKAANLYTQAASATAYMVPEILSIQEEKLQQFLLEKEELKLYSHALEEINKERPHVLSEE
EEGILAEASDVLSSPSNTFGMLNNADMEFPEITDENGEKVQLTHGNYITFLESENRDVRRAAFKAVYETYGRFKNTLAST
LSGAVKKDNFYAKVKHYKSAREAALSRNSIPEEVYDNLVSTINKHLPLLHRYVEIRKKVLELDEVHMYDLYTPLVKDSGM
KVTYEQAKDYMLKGLAPLGEEYSSILKEGLNNRWVDVYENKGKRSGAYSSGTYGTNPYILMNWQDNVNNLFTLVHEFGHS
VHSYYTRKYQPYPYGNYSIFVAEVASTTNEALLGEYLLNTIEDEKQRLYILNHMLEGFKGTVFRQTMFAEFEHEIHVKAQ
EGEPLTPELLTSIYYDLNKKYFGDNIEIDKEIGLEWSRIPHFYYNYYVYQYATGFSAAQALSQQILKEGKSAVDRYIEFL
KAGSSDYPIEVLKKAGVDMTSSEPIEAACKKFEEQLNEMEELLQKVNHS

Nucleotide


Download         Length: 1830 bp        

>NTDB_id=968370 MKY32_RS06365 WP_282334326.1 1273729..1275558(+) (pepF) [Bacillus sp. FSL K6-0998]
GTGGCTGAAGAAAAAAAATCGAAGAAACTGCCTTCAAGAGAAGAGGTGAAGCAAGAAGACACGTGGAGACTGGAGGATAT
CTTCCCGTCAGATGACGCTTGGAACGAGGAATTTCAAGCCGTTAAAGAACTGCTGCCCAAGCTTTCCGAATTCAAGGGCA
AGCTCGGCCATTCCGCCGATGATTTATATGAAGCGCTGACTTATCAGGATAAGGTGATGGAGCGTCTCGGGAAGCTGTAT
ACTTACGCTCATATGCGCTATGATCAGGATACGGGAAATTCTTTTTATCAAGGTCTCAATGATAAAGCGGCGAACCTCTA
TACTCAGGCTGCGAGCGCCACGGCCTACATGGTGCCGGAAATATTATCGATTCAGGAAGAAAAGCTTCAGCAGTTCCTGT
TGGAAAAAGAGGAATTAAAGCTTTACTCACACGCTCTCGAAGAAATCAATAAAGAGCGCCCTCATGTGCTTAGCGAGGAA
GAGGAAGGGATTTTGGCCGAAGCGTCTGATGTTCTTTCATCTCCTTCCAATACATTCGGCATGCTGAATAACGCCGATAT
GGAATTTCCGGAGATCACCGATGAAAACGGAGAAAAGGTGCAGCTCACACACGGCAATTATATCACCTTTTTGGAAAGCG
AGAACCGCGATGTCCGCCGCGCGGCATTCAAGGCCGTTTATGAAACATACGGCCGCTTTAAAAACACGCTAGCTTCCACG
CTCAGCGGAGCTGTGAAAAAAGACAACTTTTATGCGAAGGTTAAACATTATAAATCGGCGCGGGAAGCTGCCTTGTCGCG
CAACAGCATTCCTGAAGAAGTCTACGACAATCTTGTCAGCACGATCAACAAGCATCTCCCGCTTCTTCACCGCTATGTTG
AGATTAGAAAAAAAGTGCTTGAGCTTGATGAAGTGCACATGTATGACCTGTATACCCCTCTGGTCAAGGATTCTGGAATG
AAAGTCACTTACGAGCAGGCAAAGGACTATATGCTGAAGGGTCTGGCGCCTTTGGGGGAAGAATATTCATCCATCCTGAA
AGAAGGATTGAACAACCGCTGGGTCGATGTGTATGAAAACAAAGGAAAACGAAGCGGCGCCTATTCTTCAGGAACTTACG
GAACCAATCCGTATATTTTGATGAACTGGCAGGACAACGTCAACAATCTGTTTACGCTCGTCCATGAATTCGGCCATTCC
GTGCACAGCTACTATACGAGAAAATATCAGCCTTACCCGTACGGTAACTACAGCATTTTCGTAGCCGAAGTTGCTTCTAC
GACGAATGAAGCGCTGTTGGGAGAATACCTGCTGAATACAATCGAAGATGAAAAACAGCGGCTCTATATTTTGAATCATA
TGCTCGAAGGCTTTAAAGGAACGGTTTTCAGACAGACGATGTTTGCCGAATTTGAACATGAGATCCATGTCAAAGCCCAG
GAAGGGGAACCGCTGACACCTGAGCTGTTAACGAGCATATACTATGATTTGAATAAGAAATATTTCGGAGACAATATTGA
GATTGATAAGGAAATCGGTCTTGAGTGGTCAAGAATTCCGCATTTCTATTACAATTATTATGTATATCAGTATGCGACAG
GCTTCAGTGCAGCTCAGGCGCTCAGCCAGCAGATTTTAAAAGAAGGCAAATCGGCTGTTGACCGCTATATTGAGTTCTTG
AAAGCTGGAAGCTCGGATTATCCGATTGAAGTGCTGAAAAAAGCCGGCGTCGATATGACGTCTTCAGAGCCGATCGAAGC
TGCGTGCAAAAAGTTTGAAGAGCAGCTGAATGAAATGGAAGAACTCCTGCAAAAAGTCAATCATTCATAA

Domains

Predicted by InterproScan.

Oligopeptidase F (Peptidase_M3_N)

(120-189)

Peptidase family M3 (Peptidase_M3)

(210-589)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

50.754

98.03

0.498

Multiple sequence alignment