Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   WMO32_RS15950 Genome accession   NZ_CP150120
Coordinates   3535388..3536518 (-) Length   376 a.a.
NCBI ID   WP_014502536.1    Uniprot ID   G7TJ40
Organism   Xanthomonas oryzae pv. oryzicola strain TanzP11-2L     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3530388..3541518
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WMO32_RS15915 (WMO32_15915) ruvX 3530476..3530943 (-) 468 WP_341809120.1 Holliday junction resolvase RuvX -
  WMO32_RS15920 (WMO32_15920) - 3530936..3531502 (-) 567 WP_024711782.1 YqgE/AlgH family protein -
  WMO32_RS15925 (WMO32_15925) - 3531578..3532776 (+) 1199 Protein_3031 hypothetical protein -
  WMO32_RS15930 (WMO32_15930) - 3532773..3533345 (+) 573 WP_014502539.1 DNA-3-methyladenine glycosylase I -
  WMO32_RS15935 (WMO32_15935) - 3533382..3534215 (+) 834 WP_341809121.1 DUF72 domain-containing protein -
  WMO32_RS15945 (WMO32_15945) - 3534442..3535116 (-) 675 WP_024711780.1 YitT family protein -
  WMO32_RS15950 (WMO32_15950) pilU 3535388..3536518 (-) 1131 WP_014502536.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  WMO32_RS15955 (WMO32_15955) pilT 3536632..3537669 (-) 1038 WP_014502535.1 type IV pilus twitching motility protein PilT Machinery gene
  WMO32_RS15960 (WMO32_15960) - 3538035..3538727 (+) 693 WP_044750693.1 YggS family pyridoxal phosphate-dependent enzyme -
  WMO32_RS15965 (WMO32_15965) proC 3538771..3539631 (+) 861 WP_014502533.1 pyrroline-5-carboxylate reductase -
  WMO32_RS15970 (WMO32_15970) - 3540189..3540614 (+) 426 WP_014502532.1 HU family DNA-binding protein -
  WMO32_RS15975 (WMO32_15975) - 3540721..3541362 (+) 642 WP_024712272.1 M23 family metallopeptidase -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41940.17 Da        Isoelectric Point: 6.8287

>NTDB_id=962914 WMO32_RS15950 WP_014502536.1 3535388..3536518(-) (pilU) [Xanthomonas oryzae pv. oryzicola strain TanzP11-2L]
MSTIDFTSFLKLMAHQKASDLFITSGMPPAIKVNGKISPITQTPLTAQQSRDLVLNVMTPSQREEFEKTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVDELSLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGHRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWESALKNTLRQAPDVIMIGEVRAREGMDHAIAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPTPDGRSRRVAMEIMLGTPLVQDYIRDGEIHKLKEIMKESTNLGMRTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDAKTLSQGMDGVEISEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=962914 WMO32_RS15950 WP_014502536.1 3535388..3536518(-) (pilU) [Xanthomonas oryzae pv. oryzicola strain TanzP11-2L]
ATGAGCACCATCGACTTCACCTCCTTCCTCAAGCTGATGGCGCATCAGAAGGCGTCGGACCTGTTCATCACCTCAGGGAT
GCCACCGGCGATCAAGGTGAATGGCAAGATCAGCCCGATTACCCAGACGCCGCTGACTGCGCAGCAGAGCCGCGATCTGG
TGTTGAACGTGATGACGCCCTCGCAGCGCGAGGAATTCGAAAAGACCCACGAGTGCAACTTCGCCATCGGCGTTTCAGGG
GTGGGGCGTTTCCGTGTGAGCTGTTTCTACCAGCGTAATCAGGTCGGTATGGTGCTGCGCCGGATCGAAACGCGCATCCC
CACCGTGGACGAGTTGAGCCTGCCTCCGGTGATCAAGACGCTGGCGATGACCAAGCGCGGCATCATCATCTTCGTCGGCG
CCACCGGTACCGGTAAGTCGACCTCGCTGGCGGCGATGATCGGCCACCGCAACCAGAATTCCACCGGCCACATCATCACC
ATCGAAGACCCGATCGAATTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAGGTCGGCATCGATACCGACAG
CTGGGAAAGCGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGTGCCCGCGAGGGCA
TGGACCACGCGATCGCCTTCGCCGAAACCGGCCACCTGGTGCTGTGCACGCTGCACGCCAACAACGCCAATCAGGCGATG
GACCGCATCATCAACTTCTTCCCCGAAGACCGCCGCAATCAGCTGCTGATGGACCTGTCGCTCAATCTCAAGGGCGTGGT
GGCGCAGCAGCTGATTCCGACGCCGGATGGCCGCAGCCGCCGCGTTGCGATGGAAATCATGTTGGGTACGCCGCTGGTGC
AGGACTACATCCGCGACGGCGAAATCCACAAGCTCAAGGAGATCATGAAGGAGTCCACCAACCTGGGCATGCGCACCTTC
GACCAGAGCCTGTTCGAGCTCTACCAGGCTGGCGAAATCAGCTACGAAGATGCATTGCGCTACGCCGACTCGCAGAACGA
AGTGCGCTTGCGCATCAAGCTCTCGCAGGGGGGCGATGCCAAGACCTTGTCGCAGGGCATGGATGGTGTGGAGATTTCCG
AAGTTCGATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(108-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G7TJ40

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

66.485

97.606

0.649
  pilU Acinetobacter baylyi ADP1

64.986

94.947

0.617
  pilU Vibrio cholerae strain A1552

52.809

94.681

0.5
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.017

94.947

0.399
  pilT Acinetobacter nosocomialis M2

39.535

91.489

0.362
  pilT Acinetobacter baumannii D1279779

39.535

91.489

0.362
  pilT Acinetobacter baumannii strain A118

39.535

91.489

0.362