Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   WKQ99_RS26465 Genome accession   NZ_CP149965
Coordinates   5820151..5822229 (-) Length   692 a.a.
NCBI ID   WP_110602602.1    Uniprot ID   -
Organism   Pseudomonas atacamensis strain EMP42     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5815151..5827229
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WKQ99_RS26450 (WKQ99_26450) - 5816778..5818364 (-) 1587 WP_039756683.1 AAA family ATPase -
  WKQ99_RS26455 (WKQ99_26455) aroB 5818375..5819475 (-) 1101 WP_073471604.1 3-dehydroquinate synthase -
  WKQ99_RS26460 (WKQ99_26460) aroK 5819628..5820146 (-) 519 WP_024011270.1 shikimate kinase AroK -
  WKQ99_RS26465 (WKQ99_26465) pilQ 5820151..5822229 (-) 2079 WP_110602602.1 type IV pilus secretin PilQ Machinery gene
  WKQ99_RS26470 (WKQ99_26470) - 5822243..5822767 (-) 525 WP_073471601.1 pilus assembly protein PilP -
  WKQ99_RS26475 (WKQ99_26475) pilO 5822764..5823387 (-) 624 WP_243241434.1 type 4a pilus biogenesis protein PilO -
  WKQ99_RS26480 (WKQ99_26480) - 5823384..5823950 (-) 567 WP_016772051.1 PilN domain-containing protein -
  WKQ99_RS26485 (WKQ99_26485) pilM 5823950..5825014 (-) 1065 WP_024011266.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 692 a.a.        Molecular weight: 74908.16 Da        Isoelectric Point: 5.4106

>NTDB_id=962381 WKQ99_RS26465 WP_110602602.1 5820151..5822229(-) (pilQ) [Pseudomonas atacamensis strain EMP42]
MNRIFSTLGFSLWIALVSPMVMAASLKALDVAALPGDRVELKLSFDGPPPQPKGYTTESPARIALDLPGVTSQLASRNLD
LGSGNARTATVAEAKDRTRLIVSLTQLAPYSTRVEGNNLFVVVGQGASTTAPRPAAVAPRAATAAAAPARPAAQRSRAIR
GVDFQRGTEGEGNVVIDLSDPTIAPDIQEHDGKIILSFARTQLPEKLRVRLDVKDFATPVQFVNAALTGDRTVISVEPSG
TFDYSTFQTDNKLTVSVRPMSIDDLQKRNADRQAYVGDKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQN
VPWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTSAEA
KVDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSVQNKGNWNASGVN
GSSSTIGTPGSTGTNSPFVDMGTVNNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPY
QEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFSN
TQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2079 bp        

>NTDB_id=962381 WKQ99_RS26465 WP_110602602.1 5820151..5822229(-) (pilQ) [Pseudomonas atacamensis strain EMP42]
ATGAACAGGATTTTCTCCACCCTCGGTTTTTCGCTATGGATAGCGCTGGTGTCACCGATGGTAATGGCGGCCAGTCTGAA
AGCGCTGGATGTTGCCGCGCTGCCGGGTGACCGCGTCGAACTGAAGCTGTCGTTCGACGGCCCGCCACCACAGCCCAAGG
GCTACACAACCGAGTCGCCGGCGCGCATTGCACTGGATCTGCCGGGCGTGACCAGCCAGTTGGCCAGCAGGAATCTTGAT
CTGGGCAGCGGCAACGCGCGCACGGCCACGGTGGCCGAAGCCAAGGATCGCACGCGGCTGATTGTCAGCCTGACGCAATT
GGCGCCGTACAGCACGCGGGTCGAGGGCAATAATCTGTTTGTGGTGGTCGGTCAGGGCGCCTCCACGACTGCGCCGCGCC
CGGCTGCCGTTGCACCGCGTGCCGCCACGGCCGCTGCGGCACCTGCCAGACCAGCCGCGCAACGAAGCAGGGCGATTCGC
GGCGTCGATTTCCAGCGCGGCACCGAGGGCGAGGGCAACGTGGTCATTGATCTCTCTGATCCGACCATCGCCCCGGATAT
CCAGGAGCACGACGGCAAGATCATCCTCAGCTTCGCCCGTACGCAATTGCCGGAAAAGCTGCGCGTGCGCCTCGACGTCA
AGGACTTCGCCACGCCGGTGCAGTTCGTCAACGCAGCGCTCACCGGCGATCGCACCGTGATCAGTGTCGAGCCCAGCGGC
ACCTTCGACTACTCCACGTTTCAGACCGACAACAAGCTCACCGTGAGCGTGCGGCCGATGAGCATCGACGACCTGCAAAA
GCGCAACGCCGACCGTCAGGCCTATGTCGGCGACAAGCTCTCGCTGAATTTTCAGGACATCGACGTGCGTTCGGTACTGC
AACTGATCGCGGACTTCACCAACCTCAACCTCGTAGCAAGCGATACGGTGCAGGGCGGTATCACCTTGCGCCTGCAGAAC
GTGCCGTGGGATCAGGCGCTGGATCTGGTGTTGAAAACCAAGGGCCTGGACAAGCGCAAGATCGGCAATGTGCTGCTGGT
CGCGCCGGCCGATGAAATTGCCGCTCGCGAGCGCCAGGAACTGGAATCGCAGAAGCAGATCGCCGAGCTGGCGCCCCTGC
GTCGCGAGTTGCTGCAAGTCAATTACGCCAAGGCAGCGGACATCGCCAAGCTGTTCCAGTCGGTGACCAGTGCCGAGGCC
AAGGTCGACGAGCGCGGTTCGATTACCGTCGATGAGCGAACCAACAACATCATTGCCTACCAGACCCAGGATCGCCTCGA
CGAACTCCGGCGGATCGTCGCGCAGCTGGATATTCCGGTACGCCAGGTGATGATCGAGGCGCGCATCGTTGAGGCCAACG
TCGATTACGACAAGAGCCTGGGCGTGCGCTGGGGCGGTTCAGTGCAGAACAAGGGCAACTGGAACGCCTCCGGGGTCAAC
GGTTCGTCGAGCACCATCGGTACGCCGGGCAGCACCGGCACCAACTCGCCGTTCGTCGACATGGGCACGGTCAATAACAC
TTCGGGGATCGGCATCGCATTCATCACCGACAACGTGCTGCTGGATCTCGAACTGACGGCGATGGAAAAGACCGGCAACG
GCGAAATCGTCTCGCAGCCGAAAGTGGTTACCTCGGACAAGGAAACCGCGAAGATTCTTAAAGGCACCGAGATTCCCTAT
CAGGAGGCCAGCTCCAGCGGCGCGACCTCGGTGTCGTTCAAGGAGGCTTCGCTGTCACTGGAAGTTACTCCGCAGATCAC
TCCGGACAACCGCATCATCATGGAGGTCAAGGTCACCAAGGATGAGCCGGACTACCTGAACAAGGTGCAGGATGTGCCGC
CGATCAAGAAAAACGAGGTCAACGCCAAAGTGCTGGTGAATGACGGCGAGACCATCGTAATCGGCGGTGTTTTTTCAAAT
ACTCAGAGCAAGGTCGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGTGGT
TTCGGAGAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(303-349)

AMIN domain (AMIN)

(37-122)

Bacterial type II/III secretion system short domain (Secretin_N)

(377-442)

Bacterial type II and III secretion system protein (Secretin)

(527-683)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.207

100

0.747
  comQ Acinetobacter baylyi ADP1

41.787

100

0.419
  pilQ Acinetobacter baumannii D1279779

37.652

100

0.403
  pilQ Acinetobacter baumannii strain A118

37.652

100

0.403
  pilQ Legionella pneumophila strain Lp02

37.977

98.555

0.374
  pilQ Legionella pneumophila strain ERS1305867

37.962

97.832

0.371