Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   WKQ99_RS26485 Genome accession   NZ_CP149965
Coordinates   5823950..5825014 (-) Length   354 a.a.
NCBI ID   WP_024011266.1    Uniprot ID   V8RDF4
Organism   Pseudomonas atacamensis strain EMP42     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5818950..5830014
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WKQ99_RS26460 (WKQ99_26460) aroK 5819628..5820146 (-) 519 WP_024011270.1 shikimate kinase AroK -
  WKQ99_RS26465 (WKQ99_26465) pilQ 5820151..5822229 (-) 2079 WP_110602602.1 type IV pilus secretin PilQ Machinery gene
  WKQ99_RS26470 (WKQ99_26470) - 5822243..5822767 (-) 525 WP_073471601.1 pilus assembly protein PilP -
  WKQ99_RS26475 (WKQ99_26475) pilO 5822764..5823387 (-) 624 WP_243241434.1 type 4a pilus biogenesis protein PilO -
  WKQ99_RS26480 (WKQ99_26480) - 5823384..5823950 (-) 567 WP_016772051.1 PilN domain-containing protein -
  WKQ99_RS26485 (WKQ99_26485) pilM 5823950..5825014 (-) 1065 WP_024011266.1 pilus assembly protein PilM Machinery gene
  WKQ99_RS26490 (WKQ99_26490) - 5825231..5827678 (+) 2448 WP_339100685.1 penicillin-binding protein 1A -
  WKQ99_RS26495 (WKQ99_26495) - 5827919..5829187 (-) 1269 WP_016772053.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38045.57 Da        Isoelectric Point: 4.7539

>NTDB_id=962382 WKQ99_RS26485 WP_024011266.1 5823950..5825014(-) (pilM) [Pseudomonas atacamensis strain EMP42]
MLGLFNKKTNALLGIDISSTSVKLLELSRQGERYRVEAYAVEPLPANAVVEKNIAELEGVGQALSRLLVKARTGVKSVAV
AVAGSAVITKVIEMDAGLSDDELENQLKIEADQYIPYPLDEVAIDFEVQGVSPRNPERVNVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLATHLAASQERLTVAVVDIGATMTTLSVLHNGKIIYTREQLFGGRQLTEEIQRRYGLTV
EQAGLAKKQGGLPDDYVSEVLQPFREALVQQVSRSLQFFFASGQYNAVDHILLAGGTASVPGLDRLIEQRLDTPTQVANP
FADMALGSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=962382 WKQ99_RS26485 WP_024011266.1 5823950..5825014(-) (pilM) [Pseudomonas atacamensis strain EMP42]
GTGCTGGGACTCTTCAATAAAAAGACCAATGCGTTACTGGGGATCGACATCAGCTCCACGTCGGTGAAGCTGCTGGAACT
GAGCCGTCAGGGCGAGCGCTACCGGGTCGAGGCCTATGCGGTGGAGCCGTTGCCGGCGAACGCTGTGGTCGAAAAGAACA
TCGCCGAACTCGAGGGCGTTGGCCAGGCACTGAGCCGCCTGCTGGTCAAGGCGCGTACCGGGGTCAAGAGCGTGGCGGTG
GCAGTGGCGGGTTCGGCGGTGATCACCAAGGTCATCGAGATGGACGCCGGGCTGTCCGACGACGAACTGGAAAACCAGCT
CAAGATCGAGGCCGACCAATACATTCCCTATCCGCTGGACGAAGTCGCCATCGACTTTGAAGTCCAGGGCGTCTCGCCGC
GCAACCCGGAACGGGTCAACGTGCTGCTCGCCGCCTGTCGCAAGGAAAACGTCGAGGTGCGTGAAGCGGCGCTGGCGCTC
GCCGGCCTGACCGCGCGGGTGGTGGATGTCGAGGCCTACGCGCTGGAGCGCTCGTTCGGCCTGCTGGCGACGCATCTGGC
GGCCTCCCAGGAGCGCCTGACCGTGGCAGTGGTCGACATCGGTGCGACCATGACCACCCTCAGCGTCCTGCACAACGGCA
AGATCATCTACACCCGCGAGCAATTGTTCGGCGGACGCCAGCTCACCGAAGAGATCCAGCGCCGCTACGGCCTCACGGTC
GAACAGGCGGGCCTGGCGAAAAAACAGGGCGGGCTGCCTGACGATTACGTCAGCGAGGTCCTGCAGCCATTTCGCGAGGC
GCTGGTGCAGCAGGTCTCGCGCTCGCTGCAGTTCTTCTTCGCCTCCGGCCAGTACAACGCGGTCGACCATATCCTGCTGG
CCGGCGGCACCGCCTCGGTGCCCGGCCTGGACCGTCTGATCGAACAGCGTCTGGACACGCCGACGCAGGTCGCCAACCCG
TTCGCCGACATGGCTTTAGGCAGCAAGGTCAATGCTGGGGCGCTGGCCAGTGATGCGCCAGCCTTGATGATTGCCTGCGG
GCTCGCGCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB V8RDF4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.215

100

0.562
  comM Acinetobacter nosocomialis M2

56.215

100

0.562
  comM Acinetobacter baylyi ADP1

54.52

100

0.545
  pilM Legionella pneumophila strain ERS1305867

48.305

100

0.483