Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   WF513_RS15510 Genome accession   NZ_CP149803
Coordinates   3295664..3297751 (-) Length   695 a.a.
NCBI ID   WP_339083613.1    Uniprot ID   -
Organism   Pseudomonas sp. TMP9     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3290664..3302751
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WF513_RS15495 (WF513_15495) - 3292395..3293975 (-) 1581 WP_339080301.1 AAA family ATPase -
  WF513_RS15500 (WF513_15500) aroB 3293986..3295089 (-) 1104 WP_339080302.1 3-dehydroquinate synthase -
  WF513_RS15505 (WF513_15505) aroK 3295141..3295659 (-) 519 WP_339080303.1 shikimate kinase AroK -
  WF513_RS15510 (WF513_15510) pilQ 3295664..3297751 (-) 2088 WP_339083613.1 type IV pilus secretin PilQ Machinery gene
  WF513_RS15515 (WF513_15515) pilP 3297807..3298325 (-) 519 WP_339083615.1 type 4a pilus biogenesis lipoprotein PilP -
  WF513_RS15520 (WF513_15520) pilO 3298328..3298951 (-) 624 WP_339080304.1 type 4a pilus biogenesis protein PilO -
  WF513_RS15525 (WF513_15525) pilN 3298948..3299520 (-) 573 WP_339080305.1 type 4a pilus biogenesis protein PilN -
  WF513_RS15530 (WF513_15530) pilM 3299520..3300584 (-) 1065 WP_339080306.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 695 a.a.        Molecular weight: 74378.40 Da        Isoelectric Point: 4.6684

>NTDB_id=961470 WF513_RS15510 WP_339083613.1 3295664..3297751(-) (pilQ) [Pseudomonas sp. TMP9]
MNILPSFLGLSLLAALVSPSLMAADLQTLDVSALPGDRVELKLTFDEPVLAPRGYTIEQPARIALDLPGVTNKLGAKTRE
LGVGNARSVTVVEAKDRTRLIVNLTSLSPYNTRSEGNSIFIVVGEGGGNAVASNSLAPAPVAVAAKKYVPQANAISNIDF
QRGELGEGNIVITLSDASISPDIQDQGGKIRLDFAGTQLPESLRVKLDVKDFATPVQFVSATGTASKASILIEPTGVYDY
LAYQTDNKLTISVKPLTQDEAEQRKADSFSYTGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVAGNITLRLQNVPWD
QALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESKKQVADLAPLRRELIQVNYAKASDMAQLFQSVTSADGAIAE
RGSVTVDDRTNSIIAYQTQERLDELRRIIAQLDVAVRQVMIEARIVEANVDYDKALGVNWRGAGVGDNNFVVGGSGGNRP
GAPGFGQNPGTQNPTTSLGSFVDMGVEGASSGIGIGFITDNTILDLELSAMEKTGNGEIISQPKVVTSDKETAKILKGSE
IPYQEASSSGASTTSFKEAALSLEVTPQITPDNRIIMEVKVTKDAPDFARALNGVPPINKNEVNAKVLVSDGETIVLGGV
FSNTQTKGVEKVPFLGDLPYLGRMFRRDIVQDNKSELLIFLTPRIMNNQAVAVSR

Nucleotide


Download         Length: 2088 bp        

>NTDB_id=961470 WF513_RS15510 WP_339083613.1 3295664..3297751(-) (pilQ) [Pseudomonas sp. TMP9]
ATGAACATATTGCCCTCTTTTTTAGGCCTTTCCCTTTTAGCGGCACTCGTCTCGCCATCCCTTATGGCTGCAGACCTGCA
AACGCTTGATGTCTCTGCGTTACCGGGAGATCGGGTTGAGTTGAAGCTCACATTCGACGAACCTGTTCTTGCGCCTCGCG
GTTATACAATCGAGCAGCCCGCGCGTATAGCCCTTGACCTACCGGGCGTGACCAACAAGTTGGGGGCCAAGACCAGAGAA
TTAGGTGTAGGCAATGCACGAAGCGTCACTGTTGTTGAAGCGAAAGACCGGACGCGTCTTATTGTGAACCTGACTAGCTT
ATCGCCTTACAACACGCGCTCGGAAGGCAATAGCATTTTTATAGTGGTGGGTGAGGGCGGTGGTAATGCAGTCGCCAGTA
ACTCGCTGGCACCTGCTCCAGTTGCTGTGGCGGCAAAAAAATATGTACCTCAAGCCAACGCGATAAGTAACATTGATTTT
CAGCGTGGCGAGTTGGGTGAGGGTAATATTGTAATTACTCTATCTGATGCCTCGATTAGTCCTGACATACAAGATCAAGG
CGGTAAAATCCGCTTGGATTTTGCAGGCACGCAATTACCTGAGTCGCTAAGGGTAAAGCTGGATGTTAAGGATTTTGCTA
CGCCTGTTCAGTTTGTCAGCGCGACAGGCACCGCTAGCAAAGCCAGCATCCTTATCGAGCCTACCGGCGTCTATGATTAT
TTGGCTTACCAGACCGATAACAAGCTTACTATTAGCGTTAAGCCGCTAACTCAAGACGAAGCGGAGCAGCGCAAGGCTGA
TAGCTTCTCCTACACCGGTGAAAAGTTATCTCTCAATTTCCAAGATATTGATGTTCGCTCTGTTTTGCAGTTAATTGCAG
ACTTTACTGATCTGAATCTGGTTGCCAGCGATACGGTTGCTGGGAACATCACGCTGCGCTTGCAAAACGTGCCATGGGAT
CAGGCTCTTGATCTTGTGCTTAAGACTAAAGGTTTGGATAAGCGGAAAGTCGGTAATGTGTTGCTGGTTGCGCCAGCTGA
TGAGATAGCTGCACGTGAGCGTCAAGAGCTTGAGTCTAAGAAGCAAGTTGCAGACCTTGCGCCTTTGCGCCGCGAGCTTA
TACAAGTTAATTACGCTAAAGCCTCCGACATGGCTCAGTTATTCCAGTCTGTGACCAGTGCCGATGGTGCTATAGCCGAG
CGAGGCTCGGTAACTGTTGATGATCGTACCAACAGTATTATTGCCTACCAAACTCAAGAGCGACTTGATGAGCTGCGTCG
TATTATTGCGCAGCTTGACGTAGCCGTGCGGCAGGTGATGATTGAAGCGCGAATAGTGGAAGCCAATGTTGATTATGATA
AAGCGTTAGGCGTTAACTGGCGCGGTGCGGGGGTTGGCGATAATAATTTCGTAGTAGGCGGGAGTGGTGGGAATCGTCCT
GGCGCACCCGGTTTCGGACAAAATCCCGGAACCCAGAACCCAACTACATCTCTAGGGTCTTTTGTTGACATGGGTGTTGA
AGGTGCAAGCTCTGGAATAGGTATTGGTTTTATTACAGACAACACAATTCTTGATCTTGAGTTGTCAGCCATGGAGAAGA
CTGGAAACGGAGAAATTATCTCTCAGCCTAAAGTTGTTACGTCTGACAAAGAGACAGCCAAAATACTCAAGGGTTCTGAA
ATTCCCTACCAAGAGGCTAGTTCTAGCGGTGCCAGTACGACCTCATTCAAAGAGGCGGCTTTGTCCCTTGAGGTTACGCC
TCAAATCACTCCAGATAACCGAATTATCATGGAAGTTAAGGTTACTAAGGATGCGCCTGACTTTGCGCGCGCACTCAACG
GAGTGCCCCCAATCAACAAGAACGAAGTCAACGCTAAGGTTCTGGTGAGCGATGGTGAAACCATTGTGCTTGGTGGGGTC
TTCTCTAATACACAAACCAAAGGCGTTGAAAAAGTTCCTTTCCTCGGTGACCTACCTTATCTAGGCCGCATGTTCCGTCG
CGATATCGTTCAAGACAACAAGTCAGAGCTGCTAATTTTCTTGACCCCCCGCATCATGAATAATCAAGCAGTTGCGGTGA
GTCGCTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(373-438)

Secretin and TonB N terminus short domain (STN)

(299-345)

AMIN domain (AMIN)

(39-121)

Bacterial type II and III secretion system protein (Secretin)

(530-686)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

74.266

100

0.764
  comQ Acinetobacter baylyi ADP1

42.609

99.281

0.423
  pilQ Acinetobacter baumannii strain A118

41.871

100

0.419
  pilQ Acinetobacter baumannii D1279779

41.871

100

0.419
  pilQ Legionella pneumophila strain Lp02

38.358

96.403

0.37
  pilQ Legionella pneumophila strain ERS1305867

38.209

96.403

0.368