Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   WF513_RS15530 Genome accession   NZ_CP149803
Coordinates   3299520..3300584 (-) Length   354 a.a.
NCBI ID   WP_339080306.1    Uniprot ID   -
Organism   Pseudomonas sp. TMP9     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3294520..3305584
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WF513_RS15505 (WF513_15505) aroK 3295141..3295659 (-) 519 WP_339080303.1 shikimate kinase AroK -
  WF513_RS15510 (WF513_15510) pilQ 3295664..3297751 (-) 2088 WP_339083613.1 type IV pilus secretin PilQ Machinery gene
  WF513_RS15515 (WF513_15515) pilP 3297807..3298325 (-) 519 WP_339083615.1 type 4a pilus biogenesis lipoprotein PilP -
  WF513_RS15520 (WF513_15520) pilO 3298328..3298951 (-) 624 WP_339080304.1 type 4a pilus biogenesis protein PilO -
  WF513_RS15525 (WF513_15525) pilN 3298948..3299520 (-) 573 WP_339080305.1 type 4a pilus biogenesis protein PilN -
  WF513_RS15530 (WF513_15530) pilM 3299520..3300584 (-) 1065 WP_339080306.1 pilus assembly protein PilM Machinery gene
  WF513_RS15535 (WF513_15535) - 3300766..3303183 (+) 2418 WP_339080307.1 penicillin-binding protein 1A -
  WF513_RS15540 (WF513_15540) - 3303280..3304548 (-) 1269 WP_339080308.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37825.21 Da        Isoelectric Point: 4.4692

>NTDB_id=961471 WF513_RS15530 WP_339080306.1 3299520..3300584(-) (pilM) [Pseudomonas sp. TMP9]
MLGLFNKKANTLLGIDISSTSVKLLELSRSGSRYKVEAYAVEPLPPNAVVEKNIAELEGVGQALARVLAKSKSGVKTVAV
AVAGSAVITKTIEMEAGLSDDELENQLKIEADQYIPYPLEEVAIDFEVQGSSARNAERVEVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERAYGLLAEQLGGGRDELTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGKQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLQPFKEAVVQQVARSLQFFFAAGQFNDVDYILLAGGTASIPDLDRLIQQKIGTQTLVANP
FAEMALSSKVNAGALASDAPALMIACGLAMRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=961471 WF513_RS15530 WP_339080306.1 3299520..3300584(-) (pilM) [Pseudomonas sp. TMP9]
GTGCTAGGGCTCTTCAATAAGAAAGCGAATACGCTACTCGGGATCGATATCAGCTCGACCTCGGTCAAGCTCCTCGAATT
GAGCCGCTCCGGAAGCCGCTACAAGGTAGAGGCTTACGCAGTCGAGCCGCTCCCGCCAAACGCGGTGGTCGAAAAAAACA
TTGCCGAATTGGAGGGTGTTGGCCAAGCGCTTGCGCGCGTGCTCGCCAAATCCAAGAGCGGTGTAAAAACCGTGGCGGTG
GCGGTGGCCGGTTCAGCAGTGATTACCAAAACCATTGAGATGGAAGCAGGGCTTTCTGACGATGAGTTGGAAAATCAGCT
GAAGATCGAAGCTGACCAATACATCCCCTATCCGCTAGAAGAAGTGGCAATCGACTTTGAAGTGCAGGGCTCATCCGCCC
GCAACGCCGAGCGCGTTGAAGTACTGCTCGCCGCTTGCCGCAAGGAAAACGTCGAGGTTCGCGAAGCCGCTTTAGCGCTT
GCTGGCCTGACTGCAAAAGTGGTTGATGTTGAGGCGTATGCCCTTGAGCGTGCTTACGGTCTGCTAGCCGAGCAGCTGGG
TGGTGGTCGCGATGAGCTGACTGTTGCGGTTGTAGATATCGGCGCAACCATGACCACCCTCAGCGTGCTGCACAACGGTC
GCACTATCTATACGCGCGAGCAGTTATTCGGTGGCAAGCAACTAACGGAAGAAATTCAACGGCGTTATGGTTTATCCGTT
GAGGAGGCCGGTCTTGCCAAGAAGCAAGGCGGTTTACCGGATGACTATGACAGCGAAGTGCTACAGCCTTTTAAAGAGGC
GGTGGTGCAGCAGGTAGCTCGCTCCCTGCAATTTTTCTTCGCCGCCGGCCAATTCAATGACGTCGACTACATCCTGTTAG
CAGGCGGTACTGCGTCAATCCCAGATCTAGATCGTCTGATCCAACAGAAAATAGGCACCCAGACATTGGTCGCCAATCCA
TTCGCTGAAATGGCACTGAGTAGCAAGGTCAATGCAGGCGCGCTTGCCAGTGATGCGCCAGCCTTGATGATTGCTTGTGG
TCTGGCGATGAGGAGTTTCGACTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.78

100

0.568
  comM Acinetobacter nosocomialis M2

56.78

100

0.568
  comM Acinetobacter baylyi ADP1

53.672

100

0.537
  pilM Legionella pneumophila strain ERS1305867

46.61

100

0.466