Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   WDB70_RS01665 Genome accession   NZ_CP149514
Coordinates   337039..338751 (+) Length   570 a.a.
NCBI ID   WP_406665970.1    Uniprot ID   -
Organism   Gallaecimonas sp. GXIMD1310 strain aegiceras     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 332039..343751
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WDB70_RS01640 (WDB70_01640) - 332488..333024 (+) 537 WP_406665960.1 cytochrome b -
  WDB70_RS01645 (WDB70_01645) - 333076..334542 (+) 1467 WP_406665962.1 SulP family inorganic anion transporter -
  WDB70_RS01650 (WDB70_01650) - 334553..335404 (+) 852 WP_406665964.1 universal stress protein -
  WDB70_RS01655 (WDB70_01655) nadC 335453..336301 (-) 849 WP_406665966.1 carboxylating nicotinate-nucleotide diphosphorylase -
  WDB70_RS01660 (WDB70_01660) - 336556..336969 (+) 414 WP_406665968.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  WDB70_RS01665 (WDB70_01665) pilB 337039..338751 (+) 1713 WP_406665970.1 type IV-A pilus assembly ATPase PilB Machinery gene
  WDB70_RS01670 (WDB70_01670) pilC 338763..340001 (+) 1239 WP_406665972.1 type II secretion system F family protein Machinery gene
  WDB70_RS01675 (WDB70_01675) pilD 340003..340866 (+) 864 WP_406665974.1 prepilin peptidase Machinery gene
  WDB70_RS01680 (WDB70_01680) coaE 340859..341464 (+) 606 WP_406665976.1 dephospho-CoA kinase -
  WDB70_RS01685 (WDB70_01685) zapD 341503..342234 (+) 732 WP_406665978.1 cell division protein ZapD -
  WDB70_RS01690 (WDB70_01690) yacG 342245..342412 (+) 168 WP_406665980.1 DNA gyrase inhibitor YacG -
  WDB70_RS01695 (WDB70_01695) ampD 342472..343008 (+) 537 WP_406665982.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 62690.02 Da        Isoelectric Point: 5.9656

>NTDB_id=959852 WDB70_RS01665 WP_406665970.1 337039..338751(+) (pilB) [Gallaecimonas sp. GXIMD1310 strain aegiceras]
MSGFLPVAKGTLALLLRRGKINQSTAEALLKEHSSGLIPLFDVLKRSELSAADFAAFLSEQFGLPVFDLSKFDLDTIPST
LLNEQLIQKHSVLPLFKRKNRLFIATADPSNQQALEDFRFNLRLPTEAVLVNADELAKAIDKLLESSGGSLDIGDVKDDE
LADLAVDDPEEREAELNASGKEDDAPIVIYVNKILMDAIRKGASDLHFEPYEKHYRIRFRIDGILHEVAAPPVAVASRLA
ARIKVMSKLDIAERRIPQDGRIKLRLSRKKAIDFRVSTLPTLWGEKIVMRILDSSSAQLGIDMLGYDPEQKQMYLEALNR
PQGMILVTGPTGSGKTVSLYTGLNILNTEEINISTAEDPVEINLPGVNQVQIHAKAGMTFASALRSFLRQDPDVIMVGEI
RDLETAEIAIKAAQTGHLVLSTLHTNSASETLTRLVNMGVPPYNIASSVTLVIAQRLGRRLCPSCKQPEELPKDELLRQG
FSPRQIEEGFTLYRAVGCSECTGGYKGRVGIYETMAMSESLAKIIMEGGNSLDIASQAQREGVKNLRQSGLEKARQGVTS
LAEINRVTSY

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=959852 WDB70_RS01665 WP_406665970.1 337039..338751(+) (pilB) [Gallaecimonas sp. GXIMD1310 strain aegiceras]
ATGTCCGGATTCCTTCCTGTTGCCAAAGGCACCTTGGCGCTGCTACTGCGTCGAGGCAAAATCAACCAAAGTACGGCTGA
AGCTCTGTTAAAGGAACACAGCTCCGGGTTGATCCCGCTGTTTGATGTGCTCAAACGCTCCGAACTCAGTGCCGCCGATT
TTGCCGCGTTTTTGTCCGAGCAGTTTGGCCTGCCGGTCTTTGACCTCAGTAAGTTCGACCTCGATACCATTCCCAGTACG
CTTTTGAATGAGCAGCTTATTCAAAAGCATTCTGTGCTGCCGCTGTTTAAAAGAAAAAACCGCCTGTTCATTGCCACTGC
CGACCCGTCTAACCAGCAGGCGCTGGAAGACTTTCGTTTTAACCTGCGTCTGCCCACCGAGGCGGTGTTGGTTAATGCCG
ATGAACTGGCCAAGGCCATCGATAAACTGCTGGAAAGCTCCGGTGGCAGCCTGGATATCGGTGATGTCAAAGACGATGAA
CTGGCTGATCTGGCAGTAGATGACCCGGAAGAGCGGGAAGCGGAACTCAATGCCAGCGGCAAGGAAGACGATGCGCCCAT
TGTTATCTATGTGAATAAAATTCTCATGGATGCCATTCGTAAGGGCGCCTCGGATCTGCACTTTGAGCCTTATGAAAAAC
ACTACCGCATTCGCTTTCGTATCGACGGTATTTTGCATGAAGTGGCAGCGCCGCCGGTAGCGGTTGCCAGCCGCCTGGCG
GCCCGTATCAAGGTCATGTCGAAGCTCGATATTGCCGAGCGGCGTATTCCGCAAGACGGCCGTATTAAATTGCGTTTGAG
CCGCAAAAAAGCCATCGATTTTCGGGTCTCAACCCTGCCAACCCTGTGGGGCGAGAAAATCGTTATGCGTATTCTCGACT
CCAGCTCGGCGCAGCTGGGCATAGATATGCTGGGCTATGATCCGGAACAAAAGCAGATGTACCTGGAGGCCTTGAACCGG
CCGCAGGGCATGATTCTGGTAACCGGCCCCACCGGCTCCGGTAAAACGGTGTCGCTCTACACCGGCCTTAATATTCTCAA
TACCGAAGAGATTAATATTTCCACCGCCGAAGACCCGGTTGAAATCAACTTGCCGGGGGTGAATCAGGTGCAGATCCACG
CCAAGGCGGGGATGACCTTTGCCTCGGCCCTGCGCTCGTTTTTGCGTCAGGACCCGGACGTGATCATGGTCGGGGAAATT
CGTGACCTGGAAACGGCTGAAATTGCCATCAAGGCGGCGCAAACCGGTCACTTGGTGCTGTCGACTTTGCACACCAACTC
GGCTTCGGAAACCCTGACCCGTCTGGTCAATATGGGCGTGCCACCCTACAACATTGCCTCCAGTGTCACCTTGGTTATTG
CCCAGCGGCTGGGGCGGCGTTTGTGCCCGTCCTGCAAACAACCGGAAGAATTGCCAAAAGACGAGCTGTTGCGCCAGGGT
TTTTCGCCGCGGCAAATTGAAGAAGGTTTTACCCTTTACCGGGCCGTGGGGTGCAGTGAGTGCACCGGTGGTTACAAAGG
GCGAGTGGGGATCTACGAAACCATGGCGATGAGTGAGTCGCTGGCGAAGATCATTATGGAAGGTGGTAACTCGCTGGATA
TTGCCAGCCAGGCCCAACGTGAGGGCGTTAAAAACCTGCGCCAGTCGGGCCTGGAAAAGGCGCGCCAGGGCGTGACATCG
CTGGCCGAAATTAACCGCGTTACCAGTTACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(193-460)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.882

100

0.567
  pilB Vibrio cholerae strain A1552

55.536

99.825

0.554
  pilB Legionella pneumophila strain ERS1305867

58.845

94.211

0.554
  pilB Acinetobacter baumannii D1279779

59.28

92.632

0.549
  pilB Vibrio parahaemolyticus RIMD 2210633

53.792

99.474

0.535
  pilB Vibrio campbellii strain DS40M4

53.996

98.772

0.533
  pilF Neisseria gonorrhoeae MS11

49.293

99.298

0.489
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.788

96.14

0.402
  pilF Thermus thermophilus HB27

39.576

99.298

0.393
  pilB/pilB1 Synechocystis sp. PCC 6803

44.148

85.439

0.377