Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NFHSH190041_RS17605 Genome accession   NZ_AP026070
Coordinates   3952862..3954571 (+) Length   569 a.a.
NCBI ID   WP_261923012.1    Uniprot ID   -
Organism   Shewanella sp. NFH-SH190041     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3947862..3959571
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NFHSH190041_RS17575 (NFHSH190041_33500) ampE 3948452..3949303 (-) 852 WP_261923006.1 beta-lactamase regulator AmpE -
  NFHSH190041_RS17580 (NFHSH190041_33510) ampD 3949376..3949942 (-) 567 WP_261923007.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NFHSH190041_RS17585 (NFHSH190041_33520) nadC 3950154..3951014 (+) 861 WP_261923008.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NFHSH190041_RS17590 - 3951138..3951290 (+) 153 WP_261923009.1 hypothetical protein -
  NFHSH190041_RS17595 - 3951454..3951639 (+) 186 WP_261923010.1 hypothetical protein -
  NFHSH190041_RS17600 (NFHSH190041_33530) - 3952428..3952862 (+) 435 WP_261923011.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  NFHSH190041_RS17605 (NFHSH190041_33540) pilB 3952862..3954571 (+) 1710 WP_261923012.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NFHSH190041_RS17610 (NFHSH190041_33550) pilC 3954618..3955880 (+) 1263 WP_261923013.1 type II secretion system F family protein Machinery gene
  NFHSH190041_RS17615 (NFHSH190041_33560) pilD 3955956..3956873 (+) 918 WP_261923014.1 prepilin peptidase Machinery gene
  NFHSH190041_RS17620 (NFHSH190041_33570) coaE 3956934..3957554 (+) 621 WP_261923015.1 dephospho-CoA kinase -
  NFHSH190041_RS17625 (NFHSH190041_33580) zapD 3957551..3958282 (+) 732 WP_261923016.1 cell division protein ZapD -
  NFHSH190041_RS17630 (NFHSH190041_33590) yacG 3958337..3958555 (+) 219 WP_261923017.1 DNA gyrase inhibitor YacG -
  NFHSH190041_RS17635 (NFHSH190041_33600) mutT 3958552..3958950 (+) 399 WP_261923018.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 62967.36 Da        Isoelectric Point: 6.0589

>NTDB_id=95668 NFHSH190041_RS17605 WP_261923012.1 3952862..3954571(+) (pilB) [Shewanella sp. NFH-SH190041]
MPTTGLHLGLSTLFVRKALLSQEQLSGAIAKARQRRQALVSTLVQEKLIPARTIAQLCYEEYGTPLLDLDEFDLKALPED
FLNKKLIEKHRCLPLYKRGNRLYVGTSDPTNISALEDFQFSAGLHAEAILVEEDKLARALDKIQEDDVSALDLDALDESA
LAAIDIEETSRQEEEKSEDAEAPVVLYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILHEISSPPIALAARLAARL
KVMAKLDIAERRVPQDGRIKMKLSRNKSIDFRVSTLPTIWGEKIVMRILDSSSAQLGIEKLGYEPEQQAAYLEMLGKPQG
MILVTGPTGSGKTVSLYTGLNILNTPERNISTAEDPVEINLEGINQVHINQKAGLTFASALRSFLRQDPDVVMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLVNMGVPGYNIASSVNLIIAQRLARRLCRECKVPEQVPEDELRKLGFDD
AQIQSNFTLYAPHTAGCEHCSGGYKGRVGIYEVMRMSDEIARIIMAGGNSLEMANMARQQGMSDLRRSGLLKVIQGITSL
AEVNRVTSV

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=95668 NFHSH190041_RS17605 WP_261923012.1 3952862..3954571(+) (pilB) [Shewanella sp. NFH-SH190041]
ATGCCAACTACCGGCTTACACCTTGGGCTATCTACCCTCTTTGTCCGCAAGGCACTGCTTTCTCAGGAGCAGCTGAGCGG
TGCGATAGCCAAAGCCCGCCAGCGCCGTCAGGCGCTGGTGTCCACCTTAGTGCAGGAAAAACTGATACCGGCTCGCACTA
TCGCCCAGCTCTGTTACGAAGAATATGGCACGCCACTGCTGGATCTGGATGAATTTGATTTAAAAGCCCTACCGGAAGAT
TTTCTCAATAAAAAGCTAATTGAAAAGCACCGTTGTTTGCCTCTATATAAGCGTGGTAACCGTTTGTATGTTGGCACATC
CGATCCCACCAATATTTCTGCCTTAGAAGATTTCCAATTCAGTGCCGGCCTGCATGCTGAAGCTATTTTGGTGGAAGAAG
ATAAGCTAGCCCGAGCATTAGATAAAATCCAAGAAGACGACGTTAGCGCGCTCGATTTAGATGCCCTCGATGAATCAGCG
CTAGCGGCAATTGATATTGAAGAAACCAGCCGCCAGGAAGAAGAAAAAAGCGAAGATGCTGAAGCTCCTGTGGTGTTGTA
TATCAATAAAATCCTCACCGATGCGATACGCAAAGGTGCATCTGACTTGCACTTTGAACCTTACGAAAAACGTTATCGCA
TTCGTTTTCGGATCGACGGCATATTACATGAAATATCCTCACCACCTATCGCTTTAGCCGCCCGCTTAGCGGCGCGACTC
AAAGTTATGGCCAAATTAGATATTGCTGAGCGGCGCGTGCCACAAGATGGCCGCATCAAAATGAAGCTCTCCCGCAATAA
GAGTATCGACTTTCGCGTCAGTACCCTGCCGACCATCTGGGGCGAAAAAATTGTAATGCGGATCTTGGACTCTTCATCAG
CCCAGTTAGGTATTGAAAAACTAGGCTATGAACCGGAGCAGCAAGCAGCGTATCTCGAAATGCTTGGCAAGCCCCAAGGC
ATGATTCTTGTTACCGGCCCCACGGGTTCAGGCAAAACTGTTTCCCTTTATACCGGACTCAATATCCTCAACACCCCGGA
GCGCAATATCAGCACCGCCGAAGACCCAGTTGAAATTAACCTGGAAGGCATTAATCAGGTGCATATCAACCAAAAAGCTG
GGCTGACATTTGCCTCGGCATTACGCTCTTTTCTACGTCAGGATCCCGACGTGGTCATGGTCGGGGAAATACGGGATCTG
GAAACTGCTGAAATTGCCATTAAAGCCGCGCAAACAGGCCACTTGGTTCTTTCTACGCTGCACACCAACTCAGCAGCAGA
AACCTTAACCCGCTTAGTCAATATGGGGGTGCCCGGCTATAACATCGCCAGTTCAGTGAATTTAATTATCGCCCAGCGTT
TGGCGCGGCGTTTATGTCGTGAGTGTAAAGTCCCAGAGCAAGTCCCCGAGGATGAACTCAGAAAACTTGGATTTGATGAC
GCACAAATCCAAAGCAACTTTACCCTATATGCTCCCCATACGGCAGGTTGCGAACACTGCTCCGGCGGTTATAAGGGACG
GGTAGGTATCTACGAAGTGATGCGCATGAGTGATGAAATTGCTCGTATTATTATGGCTGGGGGTAATTCATTGGAAATGG
CCAATATGGCAAGGCAACAGGGCATGTCAGATCTACGTCGCTCCGGCCTACTAAAAGTTATTCAAGGGATCACCAGTTTG
GCCGAAGTTAACCGCGTCACCAGCGTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.73

99.649

0.585
  pilB Acinetobacter baumannii D1279779

57.041

98.594

0.562
  pilB Legionella pneumophila strain ERS1305867

54.225

99.824

0.541
  pilB Vibrio cholerae strain A1552

52.065

97.891

0.51
  pilB Vibrio parahaemolyticus RIMD 2210633

51.064

99.121

0.506
  pilB Vibrio campbellii strain DS40M4

49.286

98.418

0.485
  pilF Neisseria gonorrhoeae MS11

47.88

99.473

0.476
  pilF Thermus thermophilus HB27

40.564

99.649

0.404
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.462

100

0.387

Multiple sequence alignment